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T R Neblett

Publications and source records attributed to T R Neblett.

17 recordsLinked to original sources
PubMed1979

The increasing importance of polymicrobial bacteremia.

Polymicrobial bacteremia increased from 6% in 1970 to 13% in 1975 in patients with bloodstream infections. This type of serious infection most commonly complicated genitourinary (27%) and gastrointestinal (26%) conditions, frequently followed invasive procedures (68%), was more common in hospitalized patients (73%), and was often associated with malignancies (25%) or immunosuppressive or cancer chemotherapy (34%). Although polymicrobial endocarditis was more commonly encountered in recent years, this infection accounted for only 4.5% of patients with multiple organism bacteremias. Gram-negative aerobic bacteria were isolated from 62% and anaerobic bacteria in 39% of patients with polymicrobial bacteremia compared with 37% and 14%, respectively, in patients with monomicrobial bacteremia. In the 88 patients with polymicrobial bacteremia, the mortality was 44.5%, compared with 18.0% in patients with monomicrobial bacteremia.

Adolescent↗
PubMed1979

Surface topography and fine structure of the Legionnaires' disease bacterium. A study of six isolates from hospitalized patients.

Six clinical isolates of the Legionnaires' disease bacterium have been isolated from patients in two Michigan hospitals. These organisms were recovered and cultivated in vitro and the strains named by city of recovery and order of isolation Detroit 1 to 4 and Flint 1 and 2. All strains, studied by scanning and transmission electron microscopy preparations from 7-day growth on agar, showed similar characteristics. By scanning electron microscopy the cells appeared bacillary, straight, and slightly curved, and spindle shapes and forms with rounded ends were observed. Detroit 2 and 3 and Flint 1 strains showed cells with ends having reduced diameters, the cells resembling an old-fashioned rolling pin. Cell widths ranged from 0.29 to 0.39 micron, and average lengths varied from 1.60 to 2.34 microns. Filamentous forms as long as 9.8 microns were observed. Surfaces appeared quite wrinkled compared to that of an Escherichia coli control organism. As seen by transmission electron microscopy, fine structure included a multilayered cell wall characteristic of gram-negative bacteria that was irregular, reflecting wrinkling; plasma membrane; and periplasmic space. Distinct nucleoid, intracytoplasmic ribosomes and rare vacuoles were also seen. No evidence of a spore or an adversity-resistant body was observed.

Adult↗
PubMed1976

Use of droplet plating method and cystine-lactose-lactng electrolyte-deficient medium in routine quantitative urine culturing procedure.

Droplet plating of 0.01 ml of 10(-2) dilutions of mixed sonically treated urines onto cystine-lactose electrolyte-deficient agar permits formation of discrete, easily counted colonies within a small circumscribed area without interference by Proteus overswarm. Each colony is considered as arising from a single viable cell. The single dilution permits precise reproducible quantitation of urine bacteria population within the range 10(4) to 10(6) cells/ml of sample. Droplet-plated counts were found to be consistently (approximately) double those determined by standard pour plate quantitation. The method requires only inexpensive readily available materials and has been performed routinely in a large-volume clinical laboratory for several years.

Agar↗