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T Ruiz

Publications and source records attributed to T Ruiz.

41 records · Page 3Linked to original sources

Activation of yeast mannan synthetase by alpha factor pheromone.

The Saccharomyces cerevisiae mating pheromone alpha factor induces the activation of yeast mannan synthetase both in vivo and in vitro. In vivo the activating effect of the pheromone on the synthetase is specific for cells of the alpha mating type, while in vitro alpha factor is able to exert its action on the synthetase of either cell type (MAT a, MAT alpha and MAT a/MAT alpha).

Animals↗

Metabolic relationship between invertase and acid phosphatase isoenzymes in Saccharomyces cerevisiae.

Repressed cells of Saccharomyces cerevisiae, subjected to inhibition of both RNA and protein synthesis, showed a pattern of membrane-bound and cytosol acid phosphatase to the external enzyme which seemed to be linked through a precursor-product relationship. Gel exclusion chromatography did not indicate clear differences between the isoenzymes. Moreover, centrifugation experiments in CsCl and precipitation with concanavalin A suggested that there were no acid phosphatase molecules devoid of carbohydrate. Membrane-bound invertase displayed a molecular weight and a carbohydrate to protein ratio smaller than those of the exocellular enzyme. The values of molecular weight and buoyant density of the membrane-bound enzyme were closer to those found for the cytosol invertase. The stability of the level of the soluble invertase detected in the cytoplasm under derepression conditions, or after RNA or protein synthesis inhibition was found to be only apparent and represented the result of an equilibrium between synthesis and degradation.

Acid Phosphatase↗

[Graduate course at the Faculdade de Medicina de Botucatu-UNESP: survey among former students].

With the objective of evaluating teaching at the Faculdade de Medicina de Botucatu, UNESP, questionnaires were sent to 605 ex students and with return of 245 answers. The medical course was considered good, Pediatrics and Medicine obtaining the best marks, the evaluation of Medicine, however decreased among the more recently graduated. Surgery, gynecology-obstetrics and public health had a medium evaluation. Ex students were secure in diagnosis, therapeutics and evaluation of laboratory and other subsidiary texts and techniques but criticized the training in more elaborate medical procedures, essential for medical practice. The fragmentation of the curriculum into specialties was also criticized. Many of the ex students suggested as necessary measures of improvement: integration of specialties in the five basic areas of medicine and utilization of the different health services of the city for teaching.

Brazil↗

[Care according to patient gender in emergency services: different or unequal?].

It has been recently published different studies that support the idea that the health services make variability according to the patient's gender. In this sense, the aim of this study is to find out if there are gender differences in diagnostic and therapeutic care in health problems attended in emergency services. With the purpose of assessing this problem in a public hospital in Spain we have make a secondary analysis of the patient's findings got in the Emergency Service of Elche Hospital during four weeks in 1985.3, 709 patients were attended during this period. The results show a greater utilization of this service by men ORmale/female = 1.37 (1.29-1.47). The waiting time was higher for women (35 minutes) than men (29 minutes) (p = 0.0001), and also the time of getting a diagnostic (86 minutes vs 76) (p = 0.003). Diagnostic test were more frequent perform to women than men ORmale/female = 0.75 (0.64-0.85) meanwhile men receive treatment more often, ORmale/female = 1.34 (1.09-1.47). The associations are adjusted for age, diagnostic groups and patient's destination. Taking into account the possibility of more severity diseases among men that could explain the shorter waiting time and the therapeutic effort, the results show the possibility of an inequality attitude in the emergency services that would need further investigations. The more frequent use of diagnostic tests among women and consequently the longer diagnostic time, could be explain by the fact of the higher rates of nonspecific pathology.

Adolescent↗