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T Rygh

Publications and source records attributed to T Rygh.

7 recordsLinked to original sources
PubMed2000

Inhibition of aromatic L-amino acid decarboxylase activity by human autoantibodies.

A full-length rat cDNA clone encoding aromatic L-amino acid decarboxylase (AADC) (E.C. 4.1.1.28) was used for in vitro transcription and translation. The enzyme had catalytic activity (0. 2 pmol serotonin/microl lysate per min), and was stimulated 2.5-fold by the addition of excess pyridoxal phosphate. On size exclusion chromatography, AADC eluted as a single activity peak with an apparent mol. wt of 93 kD. This activity peak was immunoprecipitated by sera from patients with autoimmune polyendocrine syndrome type I (APS I) containing autoantibodies against AADC. Serum and purified IgG from these patients inhibited the enzyme activity (non-competitively) by 10-80%, while sera from APS I patients without autoantibodies and controls did not. This finding confirms and extends previous observations that APS I patients have inhibitory antibodies against key enzymes involved in neurotransmitter biosynthesis.

Adolescent↗
PubMed1999

The prevalence of hyperprolactinemia in patients with primary Sjögren's syndrome.

OBJECTIVE: To assess the prevalence of hyperprolactinemia in 55 patients with primary Sjögren's syndrome (SS), and its clinical significance. METHODS: Concentrations of serum prolactin (PRL) were determined in 55 consecutive patients with primary SS and 110 controls by a fluoroimmunometric assay in a prospective case-control design. RESULTS: The 55 patients with primary SS had higher serum PRL than 110 matching controls (271.5 vs 205.9 mIU/l; p < 0.02), and this difference was most evident in patients diagnosed before the age of 45 years (374.8 vs 245.5 mIU/l; p < 0.05), a patient population characterized by active immunological disease. Serum PRL did not correlate to disease duration, serum immunoglobulin, autoantibodies, or focus score in biopsies from minor salivary glands, but did correlate to score for internal organ disease (r = 0.33, p < 0.05). Two patients were diagnosed as having primary SS 12 years after hyperprolactinemia was first detected, and both patients had aggressive primary SS as indicated by extraglandular manifestations. One of the patients developed primary SS after being treated with bromocriptine, an inhibitor of PRL synthesis, for 12 years. CONCLUSION: Patients with primary SS have moderately increased levels of serum PRL, especially evident in patients diagnosed at a young age with active immunological disease. Serum PRL is correlated to index for internal organ disease, and primary SS may be preceded by hyperprolactinemia for many years.

Adult↗
PubMed1997

[Sjögren's syndrome. New diagnostic aspects].

European criteria for classification of Sjögren's syndrome have recently been developed and evaluated. We report the clinical and laboratory findings in 96 patients with primary Sjögren's syndrome who have been classified according to these new criteria. In our patient population the latency from appearance of the first symptom to diagnosis was 11 years. In addition to sicca symptoms in mucous membranes, the dominant symptoms were periodic fatigue (92%), arthralgia (82%), hoarse voice (71%), dry cough (54%) and diarrhoea (51%). Antibodies to the nuclear antigens SSA and SSB were found in respectively 22.2% and 15.6% of the patients. Two out of ten patients with both anti-SSA and anti-SSB antibodies gave birth to a child with heart block.

Autoantibodies↗
PubMed1988

Changes in lysosome populations in the rat kidney cortex induced by experimental proteinuria.

1. Experimental proteinuria (262.9 mg protein/24 hr urine) was induced in rats by repeated intraperitoneal injections of BSA. 2. Hypertrophy of the kidney cortex was significant 8 days after the start of the BSA injections, and the activities of lysosomal enzymes in kidney cortex and urine were significantly higher in proteinuric compared to nonproteinuric rats. 3. Lysosome populations in the kidney cortex were examined by rate sedimentation of the homogenate and by rate zonal and isopycnic centrifugation of the lysosome-rich ML fraction. 4. The activity of lysosomal enzymes in the kidney cortex increased slightly, essentially in the large, fragile lysosomes mainly recovered from the proximal tubule. 5. Proteinuria induced a shift/reduction in the density of small lysosomes from 1.235 and 1.20 g/ml to 1.225 and 1.185 g/ml, respectively. 6. Proteinuria induced a new population of small lysosomes (density 1.185 g/ml) enriched in cathepsin D.

Animals↗
PubMed1986

Analytical study on Na-K-ATPase (and cysteine insensitive p-nitrophenylphosphatase) in rat kidney-cortex microsomes subfractioned by zonal centrifugation.

The common use of Na-K-ATPase as a marker enzyme for basolateral membranes in the kidney is based on the microscopic localization of the enzyme by the cytochemical assay of Na-K-ATPase as cysteine insensitive p-nitrophenylphosphatase (Ernst S.A., J. Cell Biol. 66, 586-606, 1975). Rat kidney cortex plasma membranes were therefore fractionated by differential pelleting in isotonic sucrose, followed by equilibrium banding in linear sucrose gradients, to compare the distribution of "biochemical" and "cytochemical" assayed Na-K-ATPase. In all fractions, the distribution of Na-K-stimulated Mg-dependent ATPase differed from the distribution of cysteine insensitive p-nitrophenylphosphatase (alkaline phosphatase). Evidence is presented that this difference is not only due to the separation of plasma membranes from different cell types, but simply reflects different membrane location of the enzymic activities.

4-Nitrophenylphosphatase↗
PubMed1986

Granulocyte function in patients with multiple sclerosis.

Peripheral blood granulocytes and sera from patients with relapsing/remittent (group I) and chronic progressive (group II) multiple sclerosis (MS) did not differ from control cells and sera with regard to the proportion of cells bearing receptors for Fc gamma and C3b; random or directed (chemotactic) motility of granulocytes; activation as measured by chemiluminescence; opsonic activity of sera. The results of this study suggest that granulocyte function in MS is normal.

Cell Movement↗