Biomedical subjects

T Schlich

Publications and source records attributed to T Schlich.

11 recordsLinked to original sources

PubMed2000

[The history of organ transplantation in the mirror of the DMW].

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Ethics, Medical↗

PubMed2000

Linking cause and disease in the laboratory: Robert Koch's method of superimposing visual and 'functional' representations of bacteria.

Robert Koch based his claim that specific microorganisms cause particular diseases on laboratory studies. This paper examines how Koch set up a plausible line of argument by using special methods of representing bacteria. One kind of representation consisted in making the bacteria visible; the other mode of representation was based on disease phenomena. Using a range of techniques of isolating and controlling microorganisms, Koch combined these different modes of representation in a way that made his claims convincing. Thus, the microorganism as a specific cause of disease emerged through a chain of repeated processes of selection and representation in the laboratory.

Bacteria↗

PubMed1999

Expression of a higher plant light-harvesting chlorophyll a/b-binding protein in Synechocystis sp. PCC 6803.

A chimeric lhcb gene, coding for Lhcb, a higher plant chlorophyll a/b-binding light-harvesting complex of photosystem II (LHCII), was constructed using the Synechocystis sp. PCC 6803 psbA3 promoter and a modified lhcb gene from pea. This construct drives synthesis of full-length, mature Lhcb under the control of the strong psbA3 promoter that usually drives expression of the D1 protein of photosystem II. This chimeric gene was transformed into a photosystem I-less/chlL(-) Synechocystis sp. PCC 6803 strain that is unable to synthesize chlorophyll in darkness. In the resulting strain, a high level of lhcb transcript was detected and transcript accumulation was enhanced by addition of exogenous Zn-chlorophyllide b. The chimeric lhcb gene was translated to produce full-length Lhcb as demonstrated by pulse-labeling: a new radioactively labeled band of a size corresponding to full-length Lhcb was visible on autoradiograms. Using Triton X-114 phase fractionation, this labeled protein band was found to partition to the phase containing integral membrane proteins, indicating that the pulse-labeled Lhcb is readily integrated into the membrane. However, Lhcb was rapidly degraded and did not accumulate in thylakoid membranes to levels that were detectable other than by pulse labeling. Upon immunological detection with LHCII antibodies, a small protein (approximately 8 kDa) was found specifically in the lhcb-containing mutant. We interpret this protein to be a degradation product of the full-length Lhcb. This fragment was stabilized by supplementing cells with xanthophylls, which incorporated into thylakoid membranes only in the mutant carrying lhcb. The lutein/chlorophyll ratio of thylakoids of this mutant was about 1 : 10. These results indicate that in this cyanobacterial system Lhcb is synthesized, integrated into the membrane, and then degraded to a approximately 8 kDa fragment that is stabilized by pigment binding and does not require the presence of chlorophyll b.

Chlorophyll↗

PubMed1999

Application of the powers of compulsory admission to psychiatric hospital by general practitioners, social workers and psychiatrists.

Ways of extending or consolidating the powers of compulsory admission under the Mental Health Act 1983 have been discussed by the government and other organizations, including the Royal College of Psychiatrists. However there is little data on how existing legislation is applied. The authors examined the differential application of the Act between GPs, psychiatrists and social workers by means of an anonymous, confidential questionnaire. Fourteen case vignettes were interpreted by 20 social workers, 19 GPs and 28 Section 12 approved psychiatrists, who were asked to decide if they would detain the patient under the Act. Responses were analysed between and within the three groups. There was general agreement between groups on situations involving 'danger to self' and 'danger to others', but social workers were less likely to detain on health grounds and GPs tended to use the Act unpredictably, in areas not covered by the Act. The authors conclude that the Act may not be used to its fullest extent due to differences in interpretation or in knowledge, which may arise from differential constructs of mental illness and training. A national study of knowledge of mental health law is suggested.

Adult↗

PubMed1995

Medicalization and secularization: the Jewish ritual bath as a problem of hygiene (Germany 1820s-1840s).

In the 1820s and 1840s the Jewish ritual bath in Germany was criticized on the basis of medical arguments. Associated with this critique were demands for a change in the traditional Jewish way of life in general, especially as concerning the Jewish religion. The new role assigned to religion can be seen as part of a process of 'secularization'. The criticism of the ritual bath was justified by medical arguments and entailed a demand for an extension of the medical sphere of competence, and thus formed part of a development described as 'medicalization'. An historical investigation of the debate on the Jewish ritual bath illuminates the way in which medicalization and secularization were different aspects of the same process of the attribution of complementary circumscribed spheres of medicine and religion.

Baths↗

PubMed1995