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T Shimano

Publications and source records attributed to T Shimano.

107 records · Page 6Linked to original sources
PubMed1983

[Histopathological study on hepatic metastases and blood CEA levels in patients with intestinal carcinoma].

In 37% of 177 cases with colo-rectal cancer, our original staining method succeeded in the detection of cancer cells invaded in the small vein vessels in the primary lesion, 35.4% of which metastasized to the liver. Cancer cells were found histologically in the portal vein in these cases. Serum CEA levels were abnormally high in the cases of vein invasion in 57%. Hematogenic metastases occurred in 90% in the cases of vein invasion with abnormal CEA level. Serum CEA level decreased after the resection of the involved intestine, but increased sharply when hematogenic metastases occurred, while the CEA level increased gradually and slowly in cases of local recurrences or peritoneal disseminations .

Carcinoembryonic Antigen↗
PubMed1983

[Pancreatic cancer-related antigen (PCAA) and pancreatic tissue antigen (PaA)--their chemical compositions, tissue localization, and clinical significance].

PCAA, a glycoprotein antigen identified and purified from ascited fluid of a pancreatic cancer patient and sharing the identical immunogenicity with Gelder's POA (Shimano, 1981) and PaA, a novel pancreas tissue antigen (Loor, 1981) purified from saline extract of human pancreas were studied. (1) Chemical composition: The purified PCAA from ascites fluid of a pancreatic cancer patient has a molecular weight of 10,000,000. The same antigenic substance as PCAA was also isolated and purified from normal colonic mucosa. Amino acid compositions of these antigens were similar. PCAA appeared to be a N-glycosidic glycoprotein. Antisera against these antigens were immunologically identical to Gelder's anti-POA in gel diffusion. The purified PaA has a molecular weight of 44,000, a sedimentation coefficient of 3.4S and an isoelectric point of 4.9. (2) Tissue localization: In pancreatic cancer tissues, PCAA and PaA were detected at apical cytoplasm of cancer cells, though positive cells were in different proportions and with different distribution each other. PCAA-positive cells were well differentiated while PaA-positive cells were less differentiated in histology. (3) Serum levels: A sensitive and reproducible sandwich-type enzyme immunosorbent assay has been developed for quantitation of PCAA and PaA. Using 18.6 micrograms/ml (mean + 2 S.D. of normal controls) as an upper limit, 43 of 76 pancreatic cancer (56%) and 34 of 167 other diseases (20%) had an elevated PCAA level. Using 21.5 ng/ml as an upper limit, 50 of 76 pancreatic cancer (66%) and 13 of 167 other diseases (8%) had an elevated PaA level. The sensitivity of simultaneous assay of PCAA and PaA for the detection of pancreatic cancer increased to be 90% (69/76) while false positive was 24% (40/167). The results indicate that combination assay of PCAA and PaA may be useful in immunodiagnosis of pancreatic cancer.

Antigens, Neoplasm↗
PubMed1982

Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites.

A human pancreatic tumor cell line has been established from the ascites of a patient with histopathologically confirmed adenocarcinoma of the head of the pancreas and maintained for more than 12 months in the laboratory. Epithelioid tumor cell colonies, which resulted from primary tissue cultures of the ascitic cell component, were mechanically isolated by needle micromanipulation. Tumorigenicity was proven in athymic nude mice. Morphologically the pancreatic tumor epithelial cells grew to confluency with moderately tight adhesion to the culture plastic surface and with free-floating cells in the medium. Upon re-establishment of the tumoral xenograft in tissue culture, the epithelial cells retained their original morphology. Histologically the tumor grown in nude mice exhibited prototypic characteristics of the primary adenocarcinoma in the patient, producing abundant mucin and displaying a broad spectrum of glandular differentiation, which ranged from well to poorly differentiated adenocarcinomas with occasionally localized lymphocytic infiltrations. Furthermore, the tumor expressed carcinoembryonic antigen and human pancreas cancer associated antigen. This tumor line, designated AsPC-1, has been cultured for at least 10 passages in vitro and 3 in vivo. It represents a new model for human pancreatic cancer.

Adenocarcinoma↗
PubMed1981

Purification and characterization of a human pancreas-specific antigen.

A pancreas-specific antigen was identified by immunologic techniques and purified from saline extract of human pancreas. The purified pancreas-specific antigen was shown to be homogeneous by polyacrylamide gel electrophoresis under both denaturing and non-denaturing conditions. It had a molecular weight of 44000 as estimated by gel filtration or sodium dodecyl sulfate-gel electrophoresis, and a sedimentation coefficient of 3.4 S as analyzed by sucrose gradient centrifugation. Pancreas-specific antigen possessed an isoelectric point of 4.9 and migrated to alpha-beta region upon immunoelectrophoresis. By colorimetric assay procedures, pancreas-specific antigen exhibited no enzyme activity, such as amylase, protease, esterase, lipase, acid phosphatase, alkaline phosphatase peroxidase, deoxyribonuclease or ribonuclease. Immunoreactivity of pancreas-specific antigen was sensitive to proteolytic enzymes, perchloric acid and high temperature (70 degrees C, 10 min); but insensitive to neuraminidase or beta-glucosidase. Immunohistochemical staining revealed that pancreas-specific antigen was located in acinar cells of human pancreas. In addition, a higher concentration of pancreas-specific antigen was detected in pancreatic juice than in the saline extract of pancreas. This newly identified pancreas-specific antigen, therefore, may be a useful marker protein in physiological studies of pancreas and pancreatic secretion.

Animals↗
PubMed1981

Isolation, characterization and clinical evaluation of a pancreas cancer-associated antigen.

A pancreas cancer-associated antigen (PCAA) was identified and isolated from ascites fluid of human pancreatic cancer. Purified PCAA was homogeneous as determined by polyacrylamide gel electrophoresis. PCAA was a glycoprotein with a molecular weight of approximately 1,000,000 and consisted of 20% carbohydrates and 80% peptides, had an isoelectric point of 4.7, and migrated to alpha 2-beta region. It possessed a sedimentation coefficient of 14S and appeared to be a fibrous or fibroglobular protein. Immunoreactivity of PCAA was sensitive to proteolytic enzymes, perchloric acid, KSCN, glycine-HCl at pH 2.5, urea and lithium diiodosalicylate; and insensitive to neuraminidase or beta-glucosidase. Immunohistochemical technique revealed that PCAA was located in the cytoplasm of ductal epithelial cells of malignant pancreas. Using heteroantiserum raised against purified PCAA, horseradish peroxidase and CNBr-activated Sepharose 4B, an enzyme-immunoassay (EIA) for circulating PCAA has been developed. From a group of 40 healthy blood donors, an upper limit of 16.2 micrograms of PCAA/ml of serum has been tentatively determined. An elevated PCAA was shown in 67% (29/43) of patients with pancreas cancer, as well as in 30% (11/36) of lung cancer patients, 27% (10/37) of colonic cancer patients, and in 16% (6/36) of breast cancer patients. The reactive antigen in sera of these cancers was shown to be immunologically identical. PCAA also was detected in extracts of various human tissues, particularly pancreatic tumors, colonic tumors, and in a normal colon. Further, PCAA exhibited heterogeneity in molecular weight, isoelectric point, and electrophoretic mobility.

Animals↗
PubMed1981

Purification of a human pancreas-specific antigen.

Using an antibody reagent, an organ-specific antigen has been purified from human pancreas. Purification was achieved using ammonium sulfate and ethanol precipitations, concanavalin A-Sepharose affinity chromatography, anion-exchange chromatography and gel filtration. The antigen had a molecular weight of approximately 68,000 as determined by gel filtration, was not dissociated into subunits by sodium dodecyl sulfate, and did not bind to immobilized concanavalin A. The biological significance of this antigen in pancreatic cancer is being studied.

Animals↗
PubMed1981

Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft.

Tissue localization of a human pancreas cancer-associated antigen (PCAA) and carcinoembryonic antigen (CEA) was studied in a homologous pancreatic tumoral xenograft, a human pancreatic cancer line established from ascites (AsPC-1), with the use of the indirect immunofluorescence technique with specific anti-PCAA and anti-CEA antisera. Histologically, AsPC-1 xenograft was composed of mucinous adenocarcinoma of variable size and degree of glandular differentiation. PCAA was selectively associated with the columnar cytoplasm, was involved primarily in the epithelial proliferation, and originated at the basal aspect of the glands. In contrast, CEA appeared diffuse and was found predominantly in the cuboidal cells, with higher intensity at the luminal border and in mucin. Furthermore, growth comparison of the AsPC-1 adenocarcinoma revealed that, except in the juvenile gland that was morphologically consistent with grade 1 (G1) carcinoma in situ, the number of PCAA-positively stained cells decreased from G1 to grade 4 epithelial differentiation. In well-differentiated adenocarcinoma and degenerating adenocarcinoma, cells positive for PCAA were released into the lumen of the glands, whereas in poorly differentiated adenocarcinoma these cells were often scattered singly and/or in focal clusters and disappeared in very poorly differentiated adenocarcinoma. This study showed that the PCAA was different from CEA in its immunologic reactivity and distribution in its homologous tumoral tissues, in which PCAA was predominantly associated with the proliferative phase of the malignant epithelium. These results also indicated the potential applicability of this antigenic expression in the etiology, disease grading, and staging of human pancreatic cancer.

Adenocarcinoma↗
PubMed1980

Quantitation of prostate-specific antigen in serum by a sensitive enzyme immunoassay.

A sensitive sandwich-type enzyme immunoassay has been developed for quantitation of a human prostate-specific antigen (PA). With this method, PA at a concentration as low as 0.10 ng/ml can be detected. The assay was reproducible as within and between assays yielded a coefficient of variation of 5.7% and 4.6%, respectively. Only human prostate tissues (n = 31) were shown to contain PA. No PA was detected in other human normal or tumor tissues (n = 13). PA was not detectable in sera from normal females (n = 17) or female cancer patients (n = 25). A mean +/- S.D. of 0.47 +/- 0.661 ng/ml (ranging from less than 0.10 to 2.6) ws obtained from a group of 51 normal males. Sera from male patients with nonprostatic cancer contained a similar range of PA as that of normal males. Patients with prostate cancer (371 of 442) and benign prostatic hypertrophy (13 of 19) were shown to have elevated levels of circulating PA. Although no quantitative difference in PA levels was found between the benign prostatic hypertrophy group and Stage A of prostatic cancer, patients with Stages C and D prostatic cancer exhibited significantly elevated levels of PA qualitatively and quantitatively. These results therefore indicate that PA is a histiotypic product of the prostate and may be of use as an adjunctive tool in diagnostic procedures of prostate cancer.

Antigens↗
PubMed1977

Carcinoembryonic antigen, alpha-fetoprotein and carcinoplacental alkaline phosphatase in gastric carcinoma metastatic to the liver.

Carcinoembryonic antigen (CEA), alpha-fetoprotein (AFP), and carcinoplacental alkaline phosphatase (CPALP) were detected simultaneously in the sera and body fluids of two male patients with gastric carcinoma matestatic to the liver. At autopsy, widely disseminated gastric cancer of Borrmann III type with liver metastases was revealed in both bases. Histologically, they were moderately differentiated tubular and papillary adenocarcinomas with marked cellular atypia and necrosis. In Case 1, the properties of CPALP were identical to Nagao type CPALP, and in Case 2 the Variant type CPALP. Using immunofluorescence, CEA and CPALP were demonstrated in both primary and metastatic cells. However, only in Case 2 was AFP observed in some of the primary tumor cells.

Adenocarcinoma, Papillary↗
PubMedDate not supplied

In vivo F-19 chemical shift imaging with FTPA and antibody-coupled FMIQ.

A mixture of perfluoromethyldecahydroisoquinoline (FMIQ) emulsion coupled with anti-CEA (carcinoembryonic antigen) antibody and perfluorotripropylamine (FTPA) was injected into a nude mouse inoculated with human colon carcinoma LS174T. Three days after the injection, in vivo fluorine-19 chemical shift images of the spectral signals of the two perfluorocarbons (PFCs) were obtained simultaneously. The signal intensities and distributions of FMIQ and FTPA were similar in the liver and spleen but different in the tumor. FMIQ was detected in almost the entire tumor, with scattered areas of high uptake. FTPA, however, was detected only in the center of the tumor. The results suggest that F-19 chemical shift imaging of two PFCs, one coupled to antibody and the other not, has potential application in tumor diagnosis.

Animals↗
PubMedDate not supplied

A case of Crohn's disease with recurrent massive life-threatening hemorrhage from terminal ileum.

A case of Crohn's disease that underwent bowel resection two times at 3-year intervals due to life-threatening hemorrhage from ileum is presented. The bleeding sites were located in the ulcer lesions of ileum, in the oral side near to the ileum-colon transition. The first bleeding point was at the longitudinal ulcer of the terminal ileum and the secondary bleeding site was at the profound ulcer of ileum appearing in the oral side near to the ileo-colic anastomosis. This is the first case of Crohn's disease with recurrent life-threatening massive hemorrhage in the terminal region of ileum, for which operative bowel resections were necessary. This case suggests that we should search for the bleeding site in ileal lesions developed in the circumference of and especially the oral side near to the anastomosis due to prior resection, when intestinal massive bleeding occurs again after bowel resection, and that the careful follow-up and strict treatment with diet therapy and/or anti-inflammatory drugs are necessary for the protection of recurrence in patients with Crohn's disease.

Adult↗
PubMedDate not supplied

19F-MRI of stomach and intestine using 50% FTPA emulsion under 2 T MRI system.

1H-MRI is of clinical value in many lesions, but imaging of gastrointestinal lesions is still difficult by 1H-MRI. To overcome this weak point of 1H-MRI, rabbit stomachs were examined by 19F-MRI using 50% FTPA emulsion. We also examined the stability of 50% FTPA emulsion in the stomach and its absorption from the gastrointestinal tract. We found that 50% FTPA emulsion was very stable at pH 1.5, and only a very small amount was absorbed. A rabbit (weighing 2 kg) was anesthetized, and 100 ml of 50% FTPA emulsion was infused into the stomach by catheter. 19F-MRI was performed in this rabbit using a 2 T superconducting MRI system designed for human use, and clear pictures of the stomach were obtained. From our results we conclude that 19F-MRI of the stomach using 50% FTPA emulsion is of practical value.

Animals↗
PubMedDate not supplied

Plasminogen activation system in active even in thyroid tumors; an immunohistochemical study.

The plasminogen activation system is one of the prominent factors in tumour progression since it enhances both distant metastasis and direct invasion. In this study we investigated the expression and localization of this system in clinical thyroid tumours. It was found that urokinase-type plasminogen activator (uPA), its receptor (uPAR) and plasminogen activator inhibitor type 1 (PAI-1) were expressed diffusely in most thyroid carcinomas. The lesions positive for the latter two were equal to or sometimes even a litter more extensive than those positive for uPA. The immunoreactivity of plasminogen activator inhibitor type 2 (PAI-2), however, was often partially or even entirely absent, and only 56% of the carcinomas expressed it diffusely, indicating that the two types of inhibitors in this carcinoma have quite different characteristics. No relationship between the expression of these proteins and clinicopathological parameters could be determined. THese findings suggest that the plasminogen activation system is functionally active in thyroid carcinoma but that is has hardly any effect on its general characteristics, that is, slow growth and excellent prognosis of usual types and the exceptionally sudden progression of the anaplastic type. In benign tissues, too, this system was often expressed and appeared to enhance their growth to some extent.

Antibodies, Monoclonal↗