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T Shindo

Publications and source records attributed to T Shindo.

At least 145 records · Page 8Linked to original sources
PubMed1978

Effects of diltiazem hydrochloride (CRD-401) on hepatic, superior mesenteric and femoral hemodynamics.

The effects of d-3-acetoxy-cis-2,3-dihydro-5-[2-(dimethylamino)ethyll-2-(p-methoxyphenyl)-1,5-benzothiazepine-4(5H)-one hydrochloride (diltiazem hydrochloride; CRD-401) on the hepatic, superior mesenteric and femoral arteries were investigated in dogs. The results obtained were as follows: 1. At an i.v. dose of 100 microgram/kg, CRD-401 lowered the mean aortic blood pressure (-5%). It also decreased the diastolic aortic blood pressure (-11%) but did not affect the systolic aortic blood pressure. 2. The mean aortic blood pressure decreased after injection of CRD-401, but the blood flows in the hepatic, superior mesneteric and femoral arteries increased to 33%, 31% and 17%, respectively, more than did the control values and the vascular resistances of these arteries decreased by 25%, 27% and 17%, respectively. The present study obviously demonstrates that CRD-401 has vasodilating action on the hepatic, superior mesenteric and femoral arteries.

Animals↗
PubMed1978

Effects of diltiazem Hydrochloride (CRD-401) on renal hemodynamics of dogs.

The effect of d-3-acetoxy-cis-2,3-dihydro-5-[2-(dimethylamino)ethyl]-2-(p-methoxyphenyl)-1,5-benzothiazepine-4(5H)one hydrochloride (ditiazem hydrochloride, CRD-401) on renal hemodynamics was investigated in 18 mongrel adult dogs, and the following results were obtained. 1. Renal blood flow (RBF) was significantly increased following i.v. injection of 100 microgram/kg of CRD-401, in spite of decreased ao-tic blood pressure. Moreover the same dose of the drug elicited greater increase in RBF while the renal arteries were perfused at a constant pressure in order to prevent the decrease in systemic blood pressure from affecting RBF. These results suggest that CRD-401 has a vasodilatory action on the renal arteries. 2. The increasing rate of RBF and decreasing rate of renal vascular resistance (RVR) after injection of the drug into the renal artery at the dose level of 3 microgram/kg were found to diminish by pretreatment with hexamethonium (C6) before CRD-401. The vasodilating effect of CRD-401 on the renal arteries was therefore assumed to be more prominent in a state of renal arterial constriction. 3. RBF and RVR were increased and decreased, respectively, in proportion to the doses of CRD-401 ranging from 3 to 100 microgram/kg, the vasodilating action of CRD-401 on the renal arteries increased dose-dependently.

Animals↗
PubMed1976

Further study of the mechanism of thyroid hormone secretion in an in vitro model system: direct evidence for fusion of lysosomes with thyroglobulin liposomes.

The interaction between thyroglobulin liposomes, as a model of colloid droplets, and thyroidal lysosomes was studied in an in vitro system. On sucrose density gradient centrifugation analysis, particulate lysosomes were distributed in a high density region and were apparently clearly separated from the liposomal peak. However, when the liposomes and lysosomes were incubated together and then analyzed, the liposomes were found to migrate with the lysosomal peak. The extent of this translocation of liposomes depended on the incubation temperature: after incubations at 37 C and 0 C, 56% and 35%, respectively, of the radioactivity of liposomes was found in the lysosomal peak. This phenomenon was inhibited by 50% in the presence of 10(-3)M chlorpromazine. Matrix-labeled liposomes [125I]thyroglobulin liposomes), and membrane labeled lipolomes ([14C]-cholesterol-labeled thyroglobulin liposomes) showed similar distribution patterns in the lysosomal peak on gradient centrifugation. The liposomal thyroglobulin was hydrolyzed in a time-dependent manner in the fraction showing both radioactivity and acid phosphatase activity. These results provide direct evidence of liposome-lysosome fusion, or formation of phagolysosomes.

Animals↗