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T Sodeman

Publications and source records attributed to T Sodeman.

12 recordsLinked to original sources
PubMed2000

Bile salts mediate hepatocyte apoptosis by increasing cell surface trafficking of Fas.

Toxic bile salts induce hepatocyte apoptosis by a Fas-dependent, Fas ligand-independent mechanism. To account for this observation, we formulated the hypothesis that toxic bile salts induce apoptosis by effecting translocation of cytoplasmic Fas to the cell surface, resulting in transduction of Fas death signals. In McNtcp.24 cells the majority of Fas was cytoplasmic, as assessed by cell fractionation and immunofluorescence studies. However, cell surface Fas increased sixfold after treatment with the toxic bile salt glycochenodeoxycholate (GCDC) in the absence of increased Fas protein expression. Moreover, in cells transfected with Fas-green fluorescence protein, cell surface fluorescence also increased in GCDC-treated cells, directly demonstrating Fas translocation to the plasma membrane. Both brefeldin A, a Golgi-disrupting agent, and nocodazole, a microtubule inhibitor, prevented the GCDC-induced increase in cell surface Fas and apoptosis. In conclusion, toxic bile salts appear to induce apoptosis by promoting cytoplasmic transport of Fas to the cell surface by a Golgi- and microtubule-dependent pathway.

Apoptosis↗
PubMed1996

Organization of primary afferent axons in the trigeminal sensory root and tract of the rat.

A combination of immunocytochemical and electron microscopic methods were employed to assess the organization of the trigeminal (V) spinal tract in adult rats. Immunostaining was employed at the light microscopic level to selectively label large myelinated (by using antibodies against neurofilament protein) and small unmyelinated (by using antibodies against calcitonin gene-related peptide) primary afferents. In addition, the plant lectin Bandeiraea simplicifolia-I was employed to histochemically label small unmyelinated primary afferents. Results from these experiments indicated that larger myelinated axons were distributed throughout the cross-sectional extent of the V spinal tract (TrV), whereas smaller fibers were most numerous just below the pial surface. These results were confirmed with quantitative electron microscopy which demonstrated that the central portion of the V sensory root and TrV were composed primarily of larger myelinated fibers, whereas the periphery of the root and the portion of TrV just below the pial surface contained a higher percentage of smaller myelinated and unmyelinated axons. When considered together with results regarding the birthdates of neurochemically defined classes of V ganglion cells (White et al. [1994] J. Comp. Neurol. 350:397-411), these results suggest that TrV is laid down in a chronotopic fashion with the first axons forming its deeper portion and later arriving axons being added more superficially.

Afferent Pathways↗
PubMed1992

The workload recording method. A laboratory management tool.

To provide the physician with an accurate, timely, and useful test result remains the primary goal of the laboratory. This article reviews the workload recording method developed by the College of American Pathologists to monitor the efficiency of personnel in the laboratory. Topics discussed include historical background, time studies, implementation and data collection, productivity ratios, and management applications.

Data Collection↗
PubMed1977

Bacterial endocarditis due to Haemophilus parainfluenzae.

We have evaluated three patients with Haemophilus parainfluenzae endocarditis. Two of the three had underlying heart disease. All presented with fever, chills and malaise of less than two weeks' duration. Mitral valve involvement led to congestive heart failure in two of three cases. Treatment proved difficult, despite normally adequate dosages of antibiotics to which the pathogens were sensitive in vitro (ampicillin, 12-20 gm/dag; gentamicin, 3-5 mg/kg/day). Two patients were cured; one died. There was a suggestion of an inverse correlation between vegetation mass and favorable clinical response. Review of the English literature disclosed 22 documented cases of H parainfluenzae endocarditis, including 12 in the antibiotic era.

Adolescent↗
PubMed1974

Clinical studies on a transformation test for identification of Acinetobacter (Mima and Herellea).

Deoxyribonucleic acid (DNA) from 250 strains of aerobic, nonfermentative, gram-negative coccobacilli and rods were tested for the ability to transform a stable competent auxotroph of Acinetobacter (strain trp E 27) to prototrophy by using the method established by Juni. Several modifications of Juni's original procedure were made to adapt it for use in a clinical diagnostic laboratory. These modifications were directed primarily towards shortening the procedure to allow completion in a time framework consistent with current procedures. The modifications included changes in sterilization temperature, incubation time and temperature of the competent auxotroph and DNA preparation, overnight incubation temperature, and variations in the age of the auxotroph culture when used. Under these conditions, the transformation can easily be performed in 24 h, the final 16 to 18 h being an overnight uninterrupted incubation period. When used in conjunction with the glucose oxidative fermentative basal metabolism test, it provided a rapid highly efficient means for grouping and identifying acinetobacters which is far superior to a biochemical schema. Without exception, the 141 strains of DNA from Acinetobacter species were able to transform the auxotroph to prototrophy. None of the 105 oxidase-positive nonfermenters possessed DNA which was able to transform the Acinetobacter auxotroph to prototrophy.

Acinetobacter↗
PubMed1974

A rapid method for determining decarboxylase and dihydrolase activity.

A total of 764 fresh clinical isolates were used to test a rapid method for determining lysine, arginine, and ornithine decarboxylase activity as well as arginine dihydrolase activity. The conventional Møller decarboxylase broth was tested in parallel with the rapid method on 234 Enterobacteriaceae and 140 non-fermentative Gram-negative rods. The 0.3% agar method was tested in parallel on 245 Enterobacteriaceae and 146 non-fermentors. All media were checked at half-hour or hourly intervals for up to eight hours, with the final reading taken after incubation for 24 hours at 37 degrees C. The rapid method detected 17 positive decarboxylase or dihydrolase reactions that were not detected by the Møller broth and 16 more than the agar medium when testing Enterobacteriaceae. The corresponding figures for the nonfermentative Gram-negative rods were three and two respectively. Lysine and ornithine decarboxylase were generally detected by the rapid broth in two to four hours' incubation while the arginine decarboxylase and dihydrolase were slower and required six to eight hours. This compares with overnight incubation as the general rule for the Møller broth and agar decarboxylases. The comparable accuracy of the rapid method with conventional techniques and the shorter incubation time required for detection of positive reactions make this procedure well suited to a routine clinical laboratory.

Arginine↗
PubMed1972

Pitting function of the spleen in malaria: ultrastructural observations.

Ultrastructural studies of spleens from monkeys infected with Plasmodium knowlesi suggest that the spleen removes or "pits" malaria parasites from red cells. This function may explain the presence of nonparasitized spherocytic erythrocytes in the peripheral blood and may in part account for the discrepancy between the excessive hemolysis and the number of parasitized erythrocytes in animals with experimentally induced malaria.

Animals↗
PubMedDate not supplied

The integrated delivery system: opportunities for leadership.

The re-engineering of health care by both government and private sector efforts is dramatically changing the appearance and function of medicine. Health providers are seeking partners, stimulated by the goals of managed care organizations (MCOs) to control cost and to increase market share through regionalization of their products. Ventures between even the most unlikely of providers are developing to gain potential savings by greater economies of scale, extending services and increased regional coverage. Because of the complex nature of this process, laboratory management faces a variety of developing models under which they must operate. Pathologists face the same change. The basic elements of pathology practice change associated with integration are similar to those for hospitals (1). Integration brings with it the opportunity for cost reduction, increased market share, standardization of methods and reports, broadened services, combined infrastructure, and a contracting advantage (2). The following is a discussion of integrated delivery systems and the impact on the laboratory operation.

Community Networks↗