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T T Trujillo

Publications and source records attributed to T T Trujillo.

13 recordsLinked to original sources
PubMed1975

Biological effects of the Los Alamos meson beam on cells in culture.

Preliminary measurements of the biological effects of negative pions on cells in culture are reported. Cell survival as a function of depth was obtained by using gelatin to suspend the cells. The results indicate that the effects are more pronounced at the peak portion of negative pions. Cell-survival curves were also obtained under oxygenated and hypoxic conditions. The biological effectiveness at the peak was found to be a factor of 2.0 higher than at the plateau. The oxygen enhancement ratio was 1.5 at the peak. No significant differences in biological effects were observed above 14 mm in the peak region.

Animals↗
PubMed1969

Cell microfluorometry: a method for rapid fluorescence measurement.

A high-speed flow system for quantitative determination of fluoresence of cells containing fluorochrome has been developed. Feulgen-DNA distributions in populations of tissue culture cells and human leukocytes havebeen measured at a rate of 10(4) to 10(5) cells per minute and compare well with results of other independent methods.

Animals↗
PubMedDate not supplied

Cell classification by laser light scattering: identification and separation of unstained leukocytes.

We have used a flow-system cell sorter to separate unfixed, unstained human leukocyte cells into morphologically distinct populations based only on the intensity of 488-nm wavelength laser light simultaneously scattered by each cell at two different angles. Three populations were observed as distinct peaks in a two-parameter pulse-height distribution and were then physically sorted into separate classes and stained for cytological examination. The three groups consisted of lymphocytes, monocytes, and neutrophils. Each group contained between 77 and 98 per cent of a single cell type. Blood from an irradiated monkey was also sorted and showed the presence of a fourth peak which consisted of 61 per cent eosinophils. Thus, multiangle light-scattering information from unfixed, unstained cells may be a promising technique for rapid morphologic analysis and may have application, for example, as a highspeed automated leukocyte differential. We anticipate that this method may be useful in other clinical applications where morphologic differences are diagnostically important. One of the principal advantages of the method is elimination of fixation and staining of the samples; this is a nondestructive testing technique.

Animals↗