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T Takeuchi

Publications and source records attributed to T Takeuchi.

At least 163 records · Page 9Linked to original sources

Cytoarchitectonic study of the cerebral cortex in the horse brain--classification of the cortex area.

A cytoarchitectural classification of the horse cerebral cortex was done to determine whether functional locations exist or not such as found in the human cerebral cortex. Two adult horse brains were examined by Nissl and myelin stained methods. Six cell layers of the cerebral cortex were identified and classified into five types areas based on the cytoarchitectural organization: agranular, frontal, parietal, polar and granulous types. The agranular type was seen around the gyrus sylvius. In layer V of the agranular type, there were the many large cells that seemed like Betz cells. This type was recognized as motor area. The frontal type was seen in the lower and parietal parts of the cortex. The parietal type was seen in the rostral and occipital lobes. The polar type was seen in the frontal and dorsal-occipital sides. The granulous type was seen in the anterior-lower and posterior-lateral part of the cortex. The occupational ratios of the five types, that is, agranular, frontal, parietal, polar and granulous, to the whole cerebral cortex, were calculated by three-dimensional analysis using computer system as 19.6, 27.0, 28.9, 13.6 and 10.9%, respectively. As a result of myelin stain, horizontal fibers were seen in layer IV of parietal and polar types which looked liked the line of Gennari, therefore this area was recognized as the primary visual area.

Animals↗

[Primary squamous cell carcinoma of the prostate forming a rectourethral fistula: a case report].

Eleven cases of squamous cell carcinoma of the prostate have been previously reported in the Japanese medical literature. Patients with this type of carcinoma have dismal prognoses. Herein, we report a case in a 61-year-old man who was admitted to our hospital and whose chief complaints were dysuria and high fever. Pyuria, bacteriuria, and the results of a digital rectal examination suggested acute prostatitis. Despite the administration of antibiotics, the dysuria did not improve. A transrectal needle biopsy was performed, and histology of the tissue showed squamous cell carcinoma. After chemotherapy using methotrexate, pirarubicin, and cisplatin, total pelvic excentration was performed. At 12 months after the operation, the patient is alive with no evidence of the disease.

Antigens, Neoplasm↗

[Toxoplasmosis].

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Animals↗

[Malaria].

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Animals↗

[Cysticercosis].

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Albendazole↗

[IDDM treatment with an insulin-expressing vector].

Islet transplantation has proven successful at delivering insulin in a regulated manner. However, the scarcities of islet donors are important limitations. The idea of engineering non-beta cell such as hepatocytes, myocytes, to produce insulin emerged at the end of 80's. To attain insulin production similarly to islet beta cell, a number of devices has been performed, including proinsulin processing and regulated secretion of insulin, resulting in functioning beta-cell surrogates to some extent. These attempts will be merged with currently on-going islet-regeneration therapies from multipotent embryonic stem (ES) cells.

Animals↗

[A case of ileal conduit stenosis 13 years after construction].

A 63-year-old male was admitted to our hospital with the complaint of bilateral hydronephroses. Total cystectomy and ileal conduit construction were performed because of bladder tumor (TCC, G2, pT2N0M0) in 1985. The patient remained asymptomatic, but bilateral hydronephroses was observed by ultrasonography in 1997. DTPA renogram showed the delayed excretion. Conduitgraphy and antegrade pyelography revealed that the conduit was narrow like a pinhole at 2 cm distal region from the anastomotic site of the urinary duct. The stenotic region was inflated by a 24 Fr inflation-balloon-catheter. Chronic inflammation, which was thought to be caused by infection, was detected at the stenotic conduit by biopsy. Hydronephroses disappeared after the operation and the postoperative course was uneventful.

Catheterization↗

[Hyperdense renal cyst associated with papillary adenoma: a case report].

An abnormal shadow in the chest of a 57-year-old male was detected during a medical checkup and careful investigation revealed a left posterior mediastinal tumor (neurinoma) and a clearly demarcated homogenous mass with dimensions of 16 x 12 mm and computed tomographic (CT) value of 79 H.U. in the superior pole of the right kidney. The content solution was sticky and blackish-green. Neoplastic degenerations of 8 x 4 and 5 x 5 mm were seen in the cyst. Partial nephrectomy, which included the cystic section, was conducted and papillary adenoma was pathologically diagnosed. Nine years after the operation, the patient is alive and neither relapse nor other abnormalities were detected.

Adenoma↗

Synthesis of 6-acetamido-5-amino- and -5-guanidino-3, 4-dehydro-N-(2-ethylbutyryl)- 3-piperidinecarboxylic acids related to zanamivir and oseltamivir, inhibitors of influenza virus neuraminidases.

[reaction: see text] 6-Acetamido-5-amino- and -5-guanidino-3, 4-dehydro-N-(2-ethylbutyryl)-3-piperidinecarboxylic acids (8 and 9) have been synthesized starting from natural siastatin B, a bacterial neuraminidase inhibitor isolated from Streptomyces culture in a stereospecific fashion. These compounds are related to zanamivir and oseltamivir, inhibitors of influenza virus neuraminidases.

Acetamides↗

Mechanism of oxidative DNA damage induction in a strict anaerobe, Prevotella melaninogenica.

We investigated the mechanism of the oxidative DNA damage induction by exposure to O(2) in Prevotella melaninogenica, a strict anaerobe. Flow cytometry with hydroethidine and dichlorofluorescein diacetate showed that O(2) exposure generated O(2)*-) and H(2)O(2). Results of electron spin resonance with alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone and ethanol showed that O(2) exposure also induced *OH radical generation in P. melaninogenica loaded with FeCl(2) but not in samples without FeCl(2) loading. In P. melaninogenica, O(2) exposure increased 8-hydroxydeoxyguanosine (8OHdG), typical of oxidative DNA damage. Catalase inhibited the increase, but the *OH radical scavengers did not. Phenanthroline, a membrane-permeable Fe and Cu chelator, increased the 8OHdG induction. In FeCl(2)-loaded samples, induction of 8OHdG decreased. Addition of H(2)O(2) markedly increased 8OHdG levels. These results indicate that in P. melaninogenica, exposure to O(2) generated and accumulated O(2)* and H(2)O(2), and that a crypto-OH radical generated through H(2)O(2) was the active species in the 8OHdG induction.

8-Hydroxy-2'-Deoxyguanosine↗

Universal torsion-induced interaction from large extra dimensions

We consider the Kaluza-Klein (KK) scenario in which only gravity exists in the bulk. Without the assumption of symmetric connection, the presence of brane fermions induces torsion. The result is a universal axial contact interaction that dominates those induced by KK gravitons. This enhancement arises from a large spin density on the brane. Using a global fit to Z-pole observables, we find the 3sigma bound on the scale of quantum gravity to be 28 TeV for n = 2. If Dirac or light sterile neutrinos are present, the data from SN1987A increase the bound to sqrt[n] M(S)>/=210 TeV.

Journal Article↗

E-73, an acetoxyl analogue of cycloheximide, blocks the tumor necrosis factor-induced NF-kappaB signaling pathway.

Proinflammatory cytokines such as tumor necrosis factor (TNF) and interleukin (IL)-1 activate the NF-kappaB signaling pathway which induces the expression of a variety of genes such as the encoding intercellular adhesion molecule (ICAM)-1. We have found that E-73, an acetoxyl analogue of cycloheximide, specifically blocks TNF-induced ICAM-1 expression even at concentrations unable to affect protein synthesis. By contrast, cycloheximide inhibited both TNF- and IL-1-induced ICAM-1 expression primarily due to the blockage of protein synthesis. The nuclear translocation of NF-kappaB as well as the IkappaB degradation induced by TNF, but not by IL-1, was significantly prevented by E-73. These observations suggest that E-73 blocks the TNF-induced NF-kappaB signaling pathway upstream of IkappaB degradation.

Blotting, Western↗

Cellular localization of membrane-type serine protease 1 and identification of protease-activated receptor-2 and single-chain urokinase-type plasminogen activator as substrates.

Membrane-type serine protease 1 (MT-SP1) was recently cloned, and we now report its biochemical characterization. MT-SP1 is predicted to be a type II transmembrane protein with an extracellular protease domain. This localization was experimentally verified using immunofluorescent microscopy and a cell-surface biotinylation technique. The substrate specificity of MT-SP1 was determined using a positional scanning-synthetic combinatorial library and substrate phage techniques. The preferred cleavage sequences were found to be (P4-(Arg/Lys)P3-(X)P2-(Ser)P1-(Arg)P1'-(Ala)) and (P4-(X)P3-(Arg/Lys)P2-(Ser)P1(Arg) P1'(Ala)), where X is a non-basic amino acid. Protease-activated receptor 2 (PAR2) and single-chain urokinase-type plasminogen activator are proteins that are localized to the extracellular surface and contain the preferred MT-SP1 cleavage sequence. The ability of MT-SP1 to activate PARs was assessed by exposing PAR-expressing Xenopus oocytes to the soluble MT-SP1 protease domain. The latter triggered calcium signaling in PAR2-expressing oocytes at 10 nm but failed to trigger calcium signaling in oocytes expressing PAR1, PAR3, or PAR4 at 100 nm. Single-chain urokinase-type plasminogen activator was activated using catalytic amounts of MT-SP1 (1 nm), but plasminogen was not cleaved under similar conditions. The membrane localization of MT-SP1 and its affinity for these key extracellular substrates suggests a role of the proteolytic activity in regulatory events.

Animals↗