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Biomedical subjects

T Takimoto

Publications and source records attributed to T Takimoto.

At least 163 records · Page 9Linked to original sources
PubMed1982

Nasopharyngeal carcinoma in Japan--clinical evaluation of EBV serology and EBV-associated nuclear antigens test.

This study was aimed at the possible clinical application of two Epstein-Barr virus (EBV) immunological tests to Japanese nasopharyngeal carcinoma (NPC). One of them was serological test to measure antibody titers to EBV-associated antigens (viral capsid antigens (VCA) and virus-induced early antigens (EA)) of 42 NPC sera. The other was for the detection of EBV-associated nuclear antigens (EBNA) in 22 tumour cells of NPC. The results showed that significant elevation of antibody titers to VCA and EA which resided particularly in IgA class as well as a high incidence in the presence of EBNA were found in undifferentiated NPC. Apart from its importance in etiology, EBV serology and the EBNA test may help clinicians to establish a diagnosis of undifferentiated NPC in Japan and there is little doubt that these tests should eventually become a routine biologic test in ENT practice.

Adult↗
PubMed1981

Synaptic junctions in the cat spinal ganglion.

The cervical dorsal root ganglia (C5-C8) of the cat were examined in vivo with an electron microscope. Cell processes of unknown origin were observed to invade the area between the satellite cell sheaths and the ganglion cells, and their terminals were found to be in synaptic contact with the soma of the ganglion cells. Like chemical synapses, these synapses are numerous, containing small and round vesicles and mitochondria in the presynaptic terminals and the asymmetrical membrane thickening. The postsynaptic membrane was thicker than the presynaptic one. These presynaptic terminals were surrounded by the satellite cell sheaths. The present study strongly suggested the presence of at least a few chemical synapses, especially axosomatic synapses, in the cat spinal ganglion.

Animals↗
PubMed1980

Distribution of cerebellothalamic neurons projecting to the ventral nuclei of the thalamus: an HRP study in the cat.

Distribution of cerebellothalamic neurons projecting to the ventral nuclei of the thalamus was examined in the cat, using the horseradish peroxidase (HRP) method. After injections of HRP within the lateral or ventrolateral portions of the ventro-anterior and ventrolateral nuclear complex of the thalamus (VA-VL), neurons labeled retrogradely with HRP were seen contralaterally in the cerebellar nuclei; many of them were situated in the nucleus interpositus anterior and nucleus interpositus posterior, and a moderate number of them were located in the nucleus lateralis. Labeled neurons in the nucleus interpositus posterior were observed mainly in the medial and ventral portions of the nucleus. On the side ipsilateral to the injections, a few labeled neurons were seen in the nucleus interpositus anterior, nucleus interpositus posterior, and nucleus lateralis. Virtually no labeled neurons were found in the nucleus medialis of the cerebellum. After HRP injections into the medial or dorsomedial portions of the VA-VL, many labeled neurons were found contralaterally in the ventral and ventrolateral portions of the nucleus interpositus posterior, as well as in the nucleus lateralis, especially in its ventral and lateral portions. On the side ipsilateral to the injections, labeled neurons in the nucleus lateralis and nucleus interpositus posterior were small in number. In the nucleus medialis only a few labeled neurons were found bilaterally in the caudal levels of the nucleus. After HRP injections centered on the ventromedial nucleus of the thalamus, many labeled neurons occurred bilaterally in the caudal portions of the nucleus madialis, with a slight contralateral preponderance, and contralaterally in the lateral and ventral portions of the nucleus lateralis. A few labeled neurons were also seen contralaterally in the ventrolateral and lateral portions of the nucleus interpositus posterior, and ipsilaterally in the nucleus lateralis.

Animals↗
PubMedDate not supplied

Antinuclear antibodies in the sera of patients with nasopharyngeal carcinoma.

We studied the production of heterophile antinuclear antibodies (ANAs) in the sera of 50 patients, 20 with nasopharyngeal carcinoma (NPC) and 30 with other head and neck cancers (laryngeal cancer and maxillary cancer), before and after radiation therapy. A higher incidence of ANAs was found in the sera of patients with NPC and ANA production in these patients was higher after radiation therapy. We therefore performed in vitro experiments to explore the mechanisms of ANA production in the serum of postirradiated NPC patients. X-ray-irradiated NPC-derived cells (NPC-KT) produced a large amount of Epstein-Barr virus (NPC EBV) compared with non-irradiated NPC-KT cells. Nasopharyngeal carcinoma EBV-infected lymphocytes produced high levels of ANAs. These data suggest that lymphocytes infected by EBV from NPC cells may produce ANAs in the sera of NPC patients.

Antibodies, Antinuclear↗
PubMedDate not supplied

Epstein-Barr virus activation of human epithelial-nasopharyngeal carcinoma hybrid cells.

The authors used indirect immunofluorescence and electron microscopic techniques to study the effects of 5-iododeoxyuridine (IUDR) and the tumor promotor 12-O-tetradecanoylphorbol-13-acetate (TPA) on the epithelial hybrid cell line (NPC-KT) derived from nasopharyngeal carcinoma. The hybrid cells contained Epstein-Barr virus (EBV)-induced early antigens at a very low level but not Epstein-Barr viral capsid antigens in spontaneous production; the hybrid cells responded significantly to both IUDR and TPA induction. The chemicals induced both early antigens and viral capsid antigens in the NPC-KT cells. Moreover, the virus particles were detected in the altered NPC-KT cells by IUDR treatment. The data suggest that the EBV-induced agents activate the EBV productive cycle in a proportion of nasopharyngeal carcinoma cells.

Antigens, Viral, Tumor↗
PubMedDate not supplied

Undifferentiated endometrial carcinoma of the uterus: marked effect of chemotherapy with tetrahydropyranyl-adriamycin, paclitaxel, and carboplatin.

Undifferentiated endometrial carcinoma of the uterus is rare, and is thought to show a poor prognosis. To date, there is no consensus as to the optimal chemotherapy for this carcinoma. We report a rare case of this carcinoma in a patient who was treated surgically in combination with chemotherapy using a regimen designed by us. This chemotherapy consists of tetrahydropyranyl-adriamycin, paclitaxel, and carboplatin. This regimen is called TTJ [tetrahydropryanyl-adriamycin, taxan (paclitaxel), JM-8 (carboplatin)] chemotherapy and showed a marked effect. The patient was a 52-year-old woman with a giant tumor of the uterus measuring 28 x 18 x 13 cm and weighing 3386 g. She underwent total abdominal hysterectomy with bilateral salpingo-oophorectomy and omentectomy, but residual carcinoma remained on the surface of the small intestine. Pathologically tumor tissues comprised the whole uterus except for the uterine cervix and there were tumor tissues in the omentum. She was treated with six courses of TTJ chemotherapy without major side-effects. Currently, she remains alive without metastasis 41 months after hysterectomy. This report describes a rare case of undifferentiated endometrial carcinoma of the uterus and introduces TTJ chemotherapy resulting in the remarkable effect on this carcinoma.

Antineoplastic Combined Chemotherapy Protocols↗
PubMedDate not supplied

Epstein-Barr virus nuclear antigen-positive nasopharyngeal hybrid cells.

An epithelial-like hybrid cell line was established by cell fusion of 8-azahypoxanthine-resistant epithelial cells (Ad-AH) with lymphoblastoid cells (A2L), derived from the human nasopharynx. The nasopharyngeal hybrid cells, designated as A2L/AH, were Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA)-positive by the anticomplement immunofluorescence method. Furthermore, the treatment of the hybrid cells with 5-Iodo-2' -deoxyuridine (IUDR) induced early antigen (EA) and viral capsid antigens (VCA), while the treatment of nonproducer lymphoblastoid cells, A2L, induced EA but not VCA. The appearance of IUDR-induced VCA in the hybrid cells suggests that some factor produced by the Ad-AH cells might neutralize a repressed state of VCA and thus activate these antigens with the treatment of IUDR. These established nasopharyngeal hybrid cells might be useful for studies of in vitro nasopharyngeal carcinoma (NPC) since no EBV-carrying NPC cell lines have been established.

Antigens↗