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Biomedical subjects

T Toge

Publications and source records attributed to T Toge.

At least 217 records · Page 12Linked to original sources
PubMed1983

Induction of suppressor cell activities in normal lymphocytes by sera from gastric cancer patients.

Inductions of suppressor cell activities in normal lymphocytes by sera from 154 patients with primary gastric cancer were investigated. Sera from patients with advanced cancer induced suppressor cells which significantly depressed the proliferative responses of autologous responder lymphocytes, while those from early cancer did not. An increase in OKT8 reactive T cell populations in normal lymphocytes occurred rather than a decrease in OKT4 reactive T cells after stimulation with sera as well as concanavalin A in relation to the increase of suppressor cell activities. Furthermore, sera-induced suppressor cell activities apparently correlated with spontaneous suppressor cell activities in cancer donors and both of these activities declined after plasma exchange. These results suggest that sera from gastric cancer patients may contain factors which can induce such suppressor cell activities and that serum factors may activate the suppressor precursors to be spontaneous suppressor cells in vivo in a manner similar to that seen in vitro.

Antibodies, Monoclonal↗
PubMed1983

[Enhancement of antitumor activity of Propionibacterium avidum in combined with neurotropin in tumor bearing mice].

The enhancement of antitumor activity of Propionibacterium avidum (P. avidum) in combination with Neurotropin (NSP) was investigated in C3H mice-MH 134 tumor system. P. avidum (0.5 mg) and NSP (20 mg/kg) were administered on day 2 and from day 1 to 7 after tumor inoculation, respectively. When mice were treated with P. avidum in combination with NSP, a significant prolongation in survival days was observed (P less than 0.01). Treatment with P. avidum alone produced prolongation in survival days, but NSP did fail. Increase of Con-A induced suppressor cell activity and depressed proliferative response of spleen cells were observed by the treatment with P. avidum. However, recovery of proliferative response to normal level and disappearance of suppressor cell activity were observed when NSP was combined. Thus, treatment by P. avidum in combination with NSP produced a significant prolongation in survival days and it may be depending on macrophage activation by P. avidum and on the restoration of T cell functions by NSP.

Animals↗
PubMed1983

[Analysis of suppressor cell activities in spleen cells from gastric cancer patients and the effect of splenectomy on prognosis of gastric cancer].

In gastric cancer patients, activities of Concanavalin-A induced suppressor cells ( ConAS ) and spontaneous suppressor cells (SpS) in spleen cells (SC), peripheral blood lymphocytes (PBL) and splenic vein lymphocytes (SVL) were comparatively investigated. Suppressions by ConAS in PBL were significantly increased in patients of Stages III and IV, while suppressions by SpS were increased in patients with recurrent tumors. Elevated activities of ConAS and SpS were observed in SCs and SVLs , respectively. ConAS activities were mostly indicated in the medium sized lymphocyte fractions which were fractionated on the basis of cell size, while SpS activities in the larger sized fraction. Cell numbers in the large sized lymphocyte fraction which contained higher proportion of Tg cells and OKT8 reactive T cells, tended to increase with advances of the disease. These results suggest that spleen contained much higher proportion of suppressor precursors which might be activated to become suppressor cells with advances of the disease. Furthermore, the effect of splenectomy on the prognosis of gastric cancer was investigated in randomized controlled trial. The patients who underwent total gastrectomy and had main location of the tumor on lesser curvature region were divided into two groups at random; splenectomy (+) and splenectomy (-) groups. A suggestive prolongation of survival time was observed in splenectomy (+) group. Thus, spleen seems to contribute to the immunosuppression in gastric cancer patients and splenectomy may lead to better prognosis.

Adult↗
PubMed1983

[Enhancement of in vitro lymphocyte proliferative response by vitamin B compounds].

The effects of Vitamin B compounds consisting of B1, B6 and B12 on the in vitro lymphocyte proliferative (LP) response were investigated. Vitamin B compounds failed to enhance the LP response of healthy controls but did enhance that of cancer patients, suggesting the recovery of depressed LP response to normal level by the treatment employing the compounds. The addition of Vitamin B compounds in the generation phase of Concanavalin-A induced suppressor cells significantly abrogated the activities. However, Vitamin B compounds produced no effect to neutralize the inhibitory effect of cancer sera on LP response.

Concanavalin A↗
PubMed1982

Prognostic significance of lymphocyte proliferative responses to mitogens in gastric cancer patients.

In 501 Japanese patients with gastric cancer, the relationships between preoperative lymphocyte proliferative (LP) responses to mitogens and prognosis of patients were evaluated. Peripheral blood lymphocytes were cultured in the presence of either autologous or allogeneic serum and their LP responses to phytohemagglutinin (PHA) and pokeweed mitogen were investigated. An apparent inverse relationship between LP responses and stage of the disease was found in LP responses to PHA in the presence of autologous serum. The survival rate of patients with higher responses was significantly greater than those with lower responses, when LP responses to PHA in the presence of autologous serum served as the criterion. Probabilities of staging which were computed on the basis of LP response to PHA affirmed the reciprocal relationship between LP responses and stage of the disease. From these results, it is concluded that evaluation of LP responses may be a valuable tool in the assessment of the clinical stage and in the prediction of prognosis.

Adult↗
PubMed1982

[The relationship between serum immunosuppressive acidic protein (IAP) and cellular immunity in the patients with gastric cancer].

IAP (immunosuppressive acidic protein), one of the serum immunosuppressive factors, was investigated in the preoperative patients with gastric cancer. IAP values in sera from patients increased in accordance with advances of the disease. In the study of quantitative correlation of IAP with immunological parameters, IAP values correlated slightly with lymphocyte proliferative response to PHA, while a significant correlation between IAP values and spontaneous suppressor T cell activities was noted (p less than 0.01). These results suggested that IAP as well as immune complex might serve as a signal for the activation of suppressor T cells in vivo.

Concanavalin A↗
PubMed1981

Scanning electron microscopic studies on the surface structure of activated macrophages and on their interaction with tumor cells.

Experiments on peritoneal macrophages obtained from Corynebacterium parvum (C. parvum)-treated or untreated normal mice were performed in order to quantitate the cytotoxic activities and surface morphological modifications. The cytotoxic activities of activated macrophages were significantly higher than those or normal macrophages. There were prominent differences in surface morphology between C. parvum-activated and normal macrophages as seen by scanning electron microscopy. The activated macrophages were larger and exhibited higher surface membrane activities than normal macrophages. In interaction between macrophages and tumor cells, both activated and normal macrophages were observed to come into contact with target tumor cells during the first 24 hr. The surfaces of tumor cells entirely lost their microvilli and showed pores. Tumor cells finally died after interaction with activated macrophages for 48 hr. In contrast, no significant alterations were observed on the surfaces of tumor cells incubated with normal macrophages. In conclusion, it seems that the cytotoxic activities of macrophages correlate with the extent of macrophage activation, and the activation correlates with the surface membrane alterations.

Animals↗
PubMed1981

Effect of intracerebral administration of Corynebacterium parvum on the growth of brain tumors in mice.

The effect of intracerebral administration of Corynebacterium parvum (C. parvum) on the growth of syngeneic brain tumors in C57BL/6 mice and the mechanism of its action were investigated. Mice were inoculated with 10(4) malignant glioma cells intracerebrally, and treated with various doses of C parvum on day 6 after tumor inoculation. The growth of tumors was significantly inhibited in proportion ot the dose of C. parvum. The cytotoxic activity of effector cells was enhanced when C. parvum was administered at the site of tumor inoculation. Significant cytotoxic activity was observed in the adherent cell fraction of brain mononuclear cells, while less cytotoxic activity was observed in the nonadherent cell fraction. These results indicate that activated intracranial macrophages may participate in the tumoricidal effect of intracerebral C. parvum administration.

Animals↗
PubMed1981

Effects of surgical stress on natural killer activity, proliferative response of spleen cells and cytostatic activity of lung macrophages in rats.

The effects of surgical procedures such as thoracotomy and/or laparotomy on natural killer (NK) activity, proliferative response to phytohemagglutinin (PHA) of spleen cells and cytostatic activity of lung macrophages were investigated in rats. NK activities of rats that had undergone the surgical procedures were significantly depressed on day 1. By day 7, NK activity in the thoracotomy group exceeded that of normal rats, while the activity in the laparotomy group was similar to that in normal rats. However, NK activity in the laparothoracotomy group remained depressed. Spleen cell proliferative response to PHA was also significantly depressed on day 1 in all groups, and in the laparothoracotomy group, a depressed response was still apparent on day 14. The cytostatic activities of lung macrophages were significantly reduced in thoracotomy groups, but the effect was rather slight in the laparotomy group. It is likely that the extensive surgical stress of laparothoracotomy can depress the host's immunological reactivity and furthermore result in an enhancement of tumor growth.

Animals↗
PubMed1980

Concanavalin A-activated suppressor cell activity in gastric cancer patients.

Peripheral blood lymphocytes from both normal donors and gastric cancer patients contained suppressor cells which could be activated by concanavalin A (Con A) to suppress the proliferative response of lymphocytes from a normal donor. Con A-activated suppressor cells resided in the E rosette-forming cell fraction. An apparent inverse relationship was found between Con A-activated suppressor cell activity and lymphocyte proliferative response to phytohemagglutinin. Significant increases of suppressor cell activities were found in advanced gastric cancer patients. These activities decreased remarkably after surgical resection of the primary tumors. It is likely that nonspecific suppressor cells represent one of the major factors inducing immunosuppression in gastric cancer patients.

Adult↗
PubMed1979

Lymphocyte responsiveness to phytohemagglutinin and its modification by serum inhibitor in breast cancer patients.

Lymphocyte responsiveness in stimulation with phytohemagglutinin (PHA) and the inhibitory effect of autologous serum on lymphocyte responsiveness were investigated in 55 patients with breast cancer. Lymphocytes from patients, even in the late stage of the disease, as well as from normal healthy control, showed an ability to respond to the stimulation with PHA in the presence of pooled normal serum, while lymphocyte responsiveness in the presence of autologous serum was markedly reduced in stage III and IV patients. Similar result was found in the examination of lymphocyte responsiveness with relation to tumor size, where lymphocytes from patients with a large tumor of more than 5 cm in diameter were less responsive when cultured with autologous serum. From these results, it is reasonable to conclude that in patients with breast cancer, the apparent reduction of lymphocyte reactivity may be due to serum inhibitor that may adhere to the lymphocytes, rather than a primary lymphocyte impairment.

Adult↗
PubMed1979

Correlation between lymphocyte responsiveness to mitogens and results of chemo-immunotherapy in patients with advanced cancer.

The effect of chemo-immunotherapy on lymphocyte responsiveness to phytohemagglutinin (PHA) and pokeweed mitogen (PWM) was investigated in patients with advanced cancer. Patients received OK-432, a streptococcal preparation, intratumorally and additional systemic MFC (mitomycin-C, 5-fluorouracil, and cytosine arabinoside) chemotherapy intravenously. Patients with high lymphocyte responsiveness to mitogens at the beginning of treatment were more responsive to chemo-immunotherapy than those with low responsiveness. Furthermore, lymphocyte responsiveness to PHA was maintained at a high level throughout the treatment. In contrast, patients with initially depressed lymphocyte responsiveness maintained low responsiveness throughout the treatment. A significant increase of inhibitory activity of the serum was observed in patients with poor results of treatment. From these results, it seems reasonable to conclude that the response to chemo-immunotherapy might be predicted by in-vitro lymphocyte responsiveness.

Adult↗
PubMed1978

Evaluation of chemotherapeutic effects on gastric cancer in relation to the histological pattern.

A combination of MFC (Mitomycin-C, 5-FU and Cytosine arabinoside) was administered to 32 gastric cancer patients and study was made of the therapeutic efficacy in relation to the histological pattern. In evaluation based on the degree of cancer cell infiltration (INF), chemotherapy was more effective in cases with INF alpha and beta than those with INF gamma. As for the grade of follicular hyperplasia in regional lymph nodes of gastric cancer, the higher was the grade, the more effective was the chemotherapy. However, a significant relationship could not be observed between histological pattern of stromal reaction of the gastric cancer and clinical effects of chemotherapy. The grade of lymphocyte infiltration or connective tissue reaction in the stroma was not related with the therapeutic effects. No relationship was also obtained between the grade of sinus histiocytosis of lymphoid reaction in the lymph nodes and therapeutic effects.

Adenocarcinoma↗
PubMed1978

A case report of surgical repair of traumatic rupture of the pericardium and diaphragm.

A 55 year-old male with traumatic rupture of the pericardium, diaphragm and spleen, and with crush of the pancreas was successfully operated upon. The diagnosis of rupture of the diaphragm was done on the chest X ray on admission, and the rupture of the pericardium was diagnosed at the time of laparotomy and thoracotomy. The ruptured spleen and the crushed pancreas tail were removed, and the ruptured pericardium and diaphragm were closed successively.

Accidents, Traffic↗
PubMed1978

Mixed lymphocyte--tumor cell culture reaction in cancer patients.

To measure immunological reactivity or recognition of tumor antigens, mixed lymphocyte--tumor cell culture reaction (MLTR) was studied using a micromethod. The peripheral lymphocytes of cancer patients were mixed in vitro with autochthonous tumor cells that had been irradiated with 60Co at a dose of 6,000 rad just before use. The lymphocyte blastogenic response was estimated by the incorporation of 3H-thymidine in the acid-insoluble fraction and expressed as counts per minute. The stimulation index equal to or greater than 2.0 was arbitrarily expressed as positive blastogenic response in the present experiment. Positive blastogenic responses to autochthonous tumor cells were observed in 10 of 34 patients with various solid tumors. The intensity of blastogenic response to tumor cells was correlated with that of the non-specific responses to PHA (r=0.4732, P less than 0.01). These results indicated that non-self tumor-associated antigens, which could induce in vitro blastogenic response of autologous lymphocytes, were present in some part of human tumors.

Adult↗
PubMed1977

Comparative study of Corynebacterium parvum and Corynebacterium liquefaciens on antitumor activity against sarcoma-180.

Corynebacterium parvum and Corynebacterium liquefaciens were comparatively examined for their antitumor activity against sarcoma-180 in ddY mice. In the case of ascitic form, significant antitumor effect was observed when C. parvum was administered on days -4 and -2 or day -2. As for solid form, maximal effect was obtained when C. parvum was administered on day 0. On the other hand, C. liquefaciens exhibited maximal antitumor activity against sarcoma-180, both in ascitic and solid forms, when it was administered on days -4 and -2. However, no significant difference in respect to antitumor activity against sarcoma-180 was seen between C. parvum and C. liquefaciens (ascitic form, F1 118 = 0.09, P greater than 0.05; solid form, F1 53 = 0.03, P greater than 0.05).

Animals↗