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Biomedical subjects

T Tomono

Publications and source records attributed to T Tomono.

24 records · Page 2Linked to original sources
PubMed1982

A denuded "turn-over" deltopectoral flap combined with a latissimus dorsi myocutaneous flap in the repair of extensive radionecrosis of the chest wall.

For a one-stage repair of large and deep radionecrotic ulcers over the chest wall, we have used a denuded "turn-over" deltopectoral flap combined with a latissimus dorsi myocutaneous flap. With this technique we can easily cover a defect measuring over 20 x 20 cm. The deformity of the donor site on the contralateral infraclavicular region is minimal. Three illustrative cases are presented.

Adult↗
PubMed1981

Cleavage of human serum immunoglobulin G by an immobilized pepsin preparation.

In order to obtain an efficacious and safe immunoglobulin G (IgG) preparation for intravenous use, the digestion of IgG with an immobilized pepsin (EC 3.4.23.1) preparation was studied. Thus, pepsin was immobilized onto glutaraldehyde-activated AH-Sepharose 4B under acidic conditions. THe enzymatic properties, such as proteolytic activity, pH-activity profile and heat stability, of the immobilized pepsin preparation were examined. The immobilized pepsin retained more than 40% of its proteolytic activity toward N-acetyl-L-phenylalanyl-L-3,5-diiodo-tyrosine and more than 30% toward IgG, and also remarkable stability as compared with free pepsin. The immobilized pepsin thus prepared was efficiently used for the limited cleavage of IgG and the gel-filtration effect of the column made it easily possible to yield the F(ab')2-rich fraction for intravenous use.

Chromatography, High Pressure Liquid↗
PubMedDate not supplied

Prophylaxis of experimental HTLV-I infection in cynomolgus monkeys by passive immunization.

The protective effect of purified human immunoglobulin against human T-cell leukaemia virus type I (HTLV-I), designated ATLIG on HTLV-I infection was examined in cynomolgus monkeys (Macaca fascicularis) as a preclinical study. Passive immunization of ATLIG 24 h before challenging HTLV-I protected the monkeys from HTLV-I infection. The result suggests that passive immunization of ATLIG could provide safe and sufficient protection against HTLV-I infection in humans.

Animals↗