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T Tozawa

Publications and source records attributed to T Tozawa.

At least 19 recordsLinked to original sources
PubMed1995

[Modification of enzyme molecules due to naturally-occurring immunoglobulins linking].

The appearance of circulating enzyme-linked immunoglobulin complexes (E-Ig), that are thought to be formed because of specific interaction between circulating immunoglobulins and normal serum isoenzymes. The presence of E-Ig in an individual is regarded as a benign phenomenon, although the presence of E-Ig may result in altered enzyme activity in serum and interfere with the measurement of isoenzymes, and is thus of diagnostic importance. The present paper deals with the isoenzyme specificities of immunoglobulins isolated from E-Igs and electrophoretically abnormal enzyme components due to E-Igs. The immunoglobulins react with a single isoenzyme, certain isoenzymes only or all the isoenzymes of a given enzyme. E-Igs thus formed are themselves heterogeneous, giving rise to many different electrophoretically abnormal enzyme components.

Electrophoresis↗
PubMed1995

[The ABO blood groups-dependent reference intervals for serum alkaline phosphatase isozymes and total activity in individuals 20-39 years of age].

Reference intervals of the activity of serum alkaline phosphatase isozymes (AP-IZ: high molecular-, liver-, bone- and intestinal-types) were determined according to the ABO (blood type) system in 200 healthy subjects aged 20-39 years. AP activity was determined according to the JSCC method. AP-IZ was stained by the formazan method after isolation by TITANIII-Lipo plate electrophoresis. For the electrophoresis, treated serum with neuraminidase and untreated one were concomitantly used for detecting liver AP and bone AP respectively. As a result of comparison of mean AP-IZ activity among the groups divided according to the ABO system, total AP, intestinal AP and liver AP activities in the type B and O persons were significantly higher than in the type A and AB persons. It is well known that the activities of total AP and intestinal AP in type B and O persons are higher than in type A and AB persons, but there have been no reports showing that the activity of liver AP in type B and O persons is higher than in type A and AB persons. Furthermore, in the type B and O persons there was a low correlation (r = 0.195, p < 0.05) between the activities of liver AP and intestinal AP. The present assessment included subjects in the age group (20-39 years) considered not to show fluctuations in the activity of bone AP, which is influenced by age. The above findings should be investigated in regard to other age groups.

ABO Blood-Group System↗
PubMed1992

The first report of a case with acute myocardial infarction showing familial deficiency of creatine kinase.

A 46-year-old male patient was diagnosed as suffering from acute myocardial infarction, but his serum creatine kinase (CK) level was extremely low and no CK isozymes were detected in the serum. The total CK activities in the skeletal muscle amounted to only 2% of that of the control. Electrophoresis of the CK isozymes in the skeletal muscle showed that CK-MM was absent but the CK-BB and abnormal isozyme bands were present. There was no evidence of myocardial ischemia, although the exercise treadmill test revealed ST segment depression in the chest leads. One of the patient's sisters had an extremely low serum CK level suggesting inheritance of this abnormality. This is the first report of a case showing familial deficiency of CK.

Creatine Kinase↗
PubMed1991

Separation and characterization of sialic acid-containing salivary-type amylase from patients' sera with immunoglobulin A-type myeloma.

Isoamylases, with an abnormal anodic migration, were detected by an electrophoretic technique in the sera from two patients with immunoglobulin A-type myeloma. The abnormal isoamylase bands migrate towards the anode faster than the salivary isoamylase (S2) band and were stained more strongly than the S2 sub-band. The abnormal isoamylase could be separated from patients' sera by using size-exclusion high-performance liquid chromatography. The serum abnormal isoamylases were showed to be sialic acid residues containing amylase, after the study of treatment with neuraminidase (EC 3.2.1.18), and to be salivary-type amylase, after the study of reaction with human salivary monoclonal antibody. The abnormal bands were not detected in the saliva from one patient. The two patients had no detectable malignancies except myeloma. These findings strongly suggest that the sialic acid-containing salivary-type amylases were produced ectopically from myeloma cells. In this regard the ectopic amylase production by myeloma cells is discussed.

Aged↗
PubMed1991

Electrophoretic analysis of enzyme-linked immunoglobulins and their clinical significance.

The appearance of circulating enzyme-linked immunoglobulin complexes (E-Ig) is common for most enzymes used in clinical biochemical tests. The presence of E-Ig may result in altered enzyme activity in serum and interfere with the measurement of isoenzymes, and is thus of diagnostic importance. E-Ig can be identified by confirming that the binding protein is an immunoglobulin by its reaction with specific anti-human immunoglobulin antibodies. Currently, the presence of E-Ig in an individual is regarded as a benign phenomenon, not indicative of any particular disease process. However, it is becoming clear that E-IgG are closely associated with autoimmune states.

Autoantibodies↗
PubMed1987

Basal cell carcinoma with follicular differentiation.

A distinctive form of basal cell carcinoma with follicular differentiation was studied in 15 biopsy specimens. The neoplasms differ from other variants of basal cell carcinoma by being situated on the face only, being small and well circumscribed, and having signs of follicular differentiation, namely, numerous tiny infundibular cyst-like structures and aggregations of neoplastic cells that resemble hair follicles in telogen. The diagnostic features of basal cell carcinomas with follicular differentiation are enumerated and illustrated.

Adenocarcinoma↗
PubMed1985

[Urinary excretion of cadmium, copper and zinc in workers exposed to cadmium].

Changes in urinary cadmium, copper and zinc excretion in workers who had considerable exposure to cadmium oxide fumes were investigated over a 5-year period following the cessation of exposure. The 22 male subjects aged 22 to 55 had been welders on automobile parts for periods ranging from 7 months to 23 years, using silver solder which contained cadmium. They were divided into three groups according to levels of urinary cadmium in the first medical examination in 1975: 5 in the high excretion group, 11 in the moderate group, and 6 in the low group. Renal tubular dysfunctions were indicated in most workers in the high excretion group but not in the other two groups. The high and moderate groups showed a rapid decrease in the levels of urinary-excreted cadmium, with biological half-times of 1.42 and 1.21 years, respectively. Excreted cadmium levelled off in the two groups about 1.7 and 1.5 years after the last exposure. Urinary copper excretion in the high cadmium excretion group was about twice that in the moderate and low groups, the difference being statistically significant in comparison with the controls. Urinary zinc excretion was high in all subjects immediately after cessation of exposure but decreased rapidly. After three years it was found that the reduction of urinary zinc was greatest in the high cadmium group. Urinary cadmium showed significant correlations with both copper and zinc. Urinary copper showed a negative correlation with zinc excretion in the high cadmium excretion group and a significant positive correlation in the other two groups.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗