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Biomedical subjects

T Tsubota

Publications and source records attributed to T Tsubota.

At least 91 records · Page 5Linked to original sources
PubMed1983

Spontaneous, complete remission in adult T-cell leukemia: a case report.

A 36-year-old man with adult T-cell leukemia gradually improved to the point of complete remission without any anticancer treatment. The patient was referred to our hospital because of the appearance of morphologically abnormal lymphocytes. Thirty percent of the peripheral blood white cells were morphologically abnormal lymphocytes which had lobulated or indented nuclei, and were E rosette-forming and mature T-cell antigen-positive. No hepatosplenomegaly, lymphadenopathy or mediastinal mass was noted. He had a cough with sputum one and a half years before entry. After admission, skin nodules occurred and were diagnosed histologically as non-Hodgkin's lymphoma, diffuse pleomorphic. Serum antibodies against an adult T-cell leukemia-associated antigen were detected at a titer of 1:40. Chest X-ray films, a pulmonary function test, bronchographic examination, bronchoalveolar lavage and transbronchial lung biopsy demonstrated no relationship between the pulmonary symptoms and the adult T-cell leukemia. After treatment with antibiotics and a beclomethasone inhaler, the symptoms decreased, and then the abnormal lymphocytes and skin nodules disappeared. The spontaneous, complete remission has continued for four years.

Adult↗
PubMed1981

Hairy cell leukemia: establishment of a cell line and its characteristics.

A hairy cell leukemia (HCL) line, ZK-H, was established from peripheral blood of a 69-year-old male patient. The ZK-H cells and the patient's original hairy cells shared the same surface properties; both possessed membrane-bound IgG with kappa light chains and villous surface structures. The ZK-H line carried Epstein-Barr virus (EBV)-determined nuclear antigen, but the patient's fresh leukemic cells lacked this antigen. Morphologically, the ZK-H cells appeared lymphoblastoid and more primitive than the preculture cells. The ZK-H line had a hyperdiploid chromosome constitution of 47 and trisomy no. 2. The presence of membrane-bound immunoglobulin and of B-cell tropic EBV in this cell line provides further evidence for the B-cell nature of HCL in this patient

Aged↗
PubMed1981

14q12 translocation in a non-Burkitt lymphoma.

Chromosome analysis was performed on cells from a patient of null cell lymphoma, well-differentiated type. A 14q12 translocation was observed in all the banded cells. In addition, there were multiple chromosome abnormalities. This case will be useful in considering the significance of the 14q1(1-3) translocation in malignant lymphoma disease.

Aged↗
PubMed1980

A case of hand mirror cell variant of acute lymphoblastic leukemia.

A 30 year old female patient diagnosed as acute lymphoblastic leukemia (ALL) with hand mirror like configuration of lymphoblastic-lymphocytic cells is reported. Although the leukemia was resistant to conventional chemotherapeutic regimens, the patient always looked well and survived for more than 20 months. Surface marker analysis showed that the cell was non-T, non-B, and not reactive to antiserum against common ALL antigen. A cytogenetic study of all the analyzable metaphases of the direct bone marrow preparation had a normal female karyotype. The clinical and hematological course is described. The immunological significance and the influence of hand mirror cell on chemosensitivity and prognosis are discussed.

Adult↗
PubMed1980

Immunological distinction of adult T cell leukaemia from T cell acute lymphoblastic leukaemia.

The antigenic properties of leukaemic cells from five patients with adult T cell leukaemia were studied with rabbit anti-MOLT-4 and anti-human thymocyte antisera using indirect membrane immunofluorescent staining. The E rosette-positive, surface immunoglobulin (sIg) negative leukaemic cells from these patients gave a positive reaction with the appropriately absorbed antisera, which reacted specifically with thymocytes, cells from T cell acute lymphoblastic leukaemia (T-ALL) and T-ALL-derived lymphoblastoid cell lines (T-LCLs) and normal peripheral blood T cells. Nevertheless, the antisera further absorbed with fresh normal peripheral blood lymphocytes (FN-PBL) lost almost all the reactivities with the leukaemic cells as well as with normal peripheral blood T cells but still retained the reactivities with thymocytes, T-LCLs and T-ALL cells. The results suggest that adult T cell leukaemia cells possess a peripheral blood T cell antigen but not a thymocyte-specific antigen.

Adult↗
PubMed1979

Heterotransplantation of human leukemic B-cell, T-cell and null-cell lines in hamsters.

Human leukemic B-cell (BALL-1), T-cell (TALL-1) and null-cell (NALL-1) lines have been established from three patients with acute lymphoblastic leukemia (ALL). To study the heterotransplantability and in vivo growth characteristics, attempts were made to transplant these ALL cell lines into newborn Syrian hamsters treated with rabbit anti-hamster thymocyte serum. Intraperitoneal implantation of 1.8-3.5 x 10(7) cells gave rise to invasive tumors in all recipients after 15 to 41 days. In addition to a common in vivo feature of mesenteric and retroperitoneal tumors, BALL-1 line was characterized by infiltration of the skin, massive ascites and bone marrow invasion. TALL-1 cells infiltrated various organs including the lymph nodes, liver, gallbladder, spleen, bone marrow, central nervous system and eyes. NALL-1 line grew slowly, producing the least tumors, although there were distant metastases in the lungs. Tumor cells were detected in the blood of 2 of 3 BALL-1-bearing hamsters and in the blood of 4 of 5 TALL-1-bearing hamsters. Thus, these three ALL cell lines were found to exhibit a characteristic biological behavior in hamsters, which might be related to the different cell lineage.

Animals↗
PubMed1979

Growth characteristics of human leukemic B-cell, T-cell, and null-cell lines serially transplanted in hamsters.

Human leukemic B-cell (BALL-1), T-cell (TALL-1), and null-cell (NALL-1) lines were established from three patients with acute lymphoblastic leukemia (ALL). Intraperitoneal transplantation of these ALL cell lines into immunosuppressed newborn hamsters resulted in the development of invasive tumors in all recipients, except 4 of 10 implanted with NALL-1 line. BALL-1 and TALL-1 lines were serially transplanted for 10 and 9 passages, respectively. In addition to a common in vivo feature of mesenteric and retroperitoneal tumors, BALL-1 line was characterized by infiltration of the skin, massive ascites, and bone marrow invasion. TALL-1 cells infiltrated various organs including the lymph nodes, liver, bone marrow, central nervous system, eyes, etc. NALL-1 line grew slowly, producing the least number of tumors. Leukemic cells were detected in the blood of 24 of 25 hamsters bearing BALL-1 and in the blood of 18 of 24 hamsters bearing TALL-1. Thus, these three ALL cell lines were found to exhibit a characteristic biological behavior in hamsters.

Animals↗
PubMed1978

Comparison of the stimulating capacity of human leukemia T-cell, null cell, and B-cell lines in mixed lymphoma culture.

One-way mixed lymphocyte reactions between lymphocytes from normal human donors and mitomycin C-treated cells from human leukemia T-cell, null cell, and B-cell lines were investigated. An Epstein-Barr virus (EBV)-negative Japanese Burkitt's lymphoma line and two EBV-positive normal lymphoid cell lines were studied in parallei. Normal lymphocytes were stimulated significantly by the cultured null cells and B-cells, but only slightly by the cultured T-cells. The stimulatory capacity of these two leukemia cell lines was approximately equal to that of the lymphoma and normal B-cell lines. The results suggest that not only leukemia B-cells but also leukemia null cells have stimulatory determinants in mixed lymphocyte culture.

B-Lymphocytes↗
PubMed1978

Chronic myelocytic leukemia terminating in blast cell crisis with lymphoblastic characteristics.

A child with chronic myelocytic leukemia (CML), Philadelphia chromosome positive, developed a non-T cell, non-B cell, acute lymphocytic leukemia (ALL) during her blast cell crisis. The diagnosis was suggested by light microscopy and supported by histochemical stains and transmission electron microscopy. Immunologic studies showed the presence of a non-T, non-B leukemic blast population--indistinguishable from the most common form of ALL (null cell type). Markedly elevated terminal deoxynucleotidyl transferase (TdT) activity was found. The findings support the hypothesis that the primary cell involved in CML is a stem cell with pluripotential characteristics; frequently the blast cell proliferative phase terminates in acute myeloblastic leukemia, but it may also terminate in ALL. The TdT activity may be evidence of leukemic transformation and not necessarily related to the thymic origin of the lymphocytes.

Bone Marrow↗
PubMed1978

Profound radiosensitivity in "leukemic" T-cell lines and T-cell-type acute lymphoblastic leukemia demonstrated by sodium [51Cr]chromate labeling.

Radiation sensitivity was determined by measuring spontaneous release from 51Cr-labeled cells in various lymphoid cell populations. Among six leukemia T-cell lines originating from acute lymphoblastic leukemia, four such lines were found to be highly radiosensitive. In contrast, two of the leukemic T-cell lines and four normal control B-cell lines were not radiosensitive. Thymocytes from six patients and leukemia T-cell blasts from three patients with T-cell leukemia were likewise found to be highly radiosensitive, whereas leukemic blasts from six patients with null-cell (non-T, non-B-cell) acute lymphoblastic leukemia were not radiosensitive. Normal peripheral blood lymphocytes and mitogen-induced normal lymphoblasts were found not to be radiosensitive. The results indicate that measurement of the radiation sensitivity of acute leukemic blasts may have a therapeutic significance in coping with the heterogeneous nature of individual leukemia cases.

Animals↗