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Biomedical subjects

T Tsuru

Publications and source records attributed to T Tsuru.

At least 91 records · Page 5Linked to original sources
PubMed1984

[Clinicopathological study on metaplasia in the bile duct mucosa with special reference to bile duct carcinoma].

Metaplasia of bile duct epithelium was studied clinicopathologically in 36 autopsy cases (Group-A), 18 surgical cases of obstructive jaundice without bile duct carcinoma (Group-B) and 56 surgical cases of bile duct carcinoma (Group-C). The results were as follows: In Group-A there were 24 cases with metaplastic pyrolic glands, and no metaplastic goblet cells. In group-B, there were 16 cases with metaplastic pyrolic glands, 2 cases with metaplastic goblet cells and 1 case with enterochromaffin cells. The mucosa of non-tumorous region of Group-C had metaplastic pyrolic glands in 46 cases, metaplastic goblet cells in 14 cases, enterochromaffin cells in 2 cases and intestinal absorbtive epithelia in 5 cases. In the tumorous region, however, tumor cells with goblet vesicles (malignant goblet cells) were seen in 31 cases, malignant intestinal absorptive villi were in 18 cases, malignant enterochromaffin cells were in 8 cases. Thirty one cases with malignant goblet cells were classified as intestinal type of the bile duct carcinoma (I-type). And 25 cases without malignant goblet cells were classified as non-intestinal type of the bile duct carcinoma (non-I-type). Conclusively, these clinicopathological findings suggest that I-type and non-I-type in the carcinoma of the bile duct are identifiable and I-type in the carcinoma of the bile duct are identifiable and I-type may arise from the lesion with intestinal metaplasia of the benign epithelia of the duct.

Adenocarcinoma, Papillary↗
PubMed1984

Endothelial wound-healing of monkey cornea: fluorophotometric and specular microscopic studies.

The process of wound-healing in the corneal endothelium after transcorneal freezing was studied in the monkey, using the techniques of fluorophotometry, pachymetry and specular microscopy. After intramuscular injection of fluorescein, the dye exchange between the cornea and aqueous humor was studied by fluorophotometry and the results were analyzed on the basis of a two-compartment model: the transfer coefficient (kac) and the distribution ratio of the dye between the aqueous humor and the cornea (rac) were calculated, and the endothelial permeability coefficient (Kac) was computed using the thickness of the corneal stroma. The corneal thickness was measured at its center (CT) with a modified pachymeter. The endothelial cell morphometry was carried out at the central cornea using a wide-field contact-type specular microscope and a computerized image analyzer: the mean cell size (CS) was calculated. The values of Kac, kac, rac, CT and CS in 16 normal uninjured corneas were 3.35 +/- 0.91 X 10(-4) cm min-1, 0.56 +/- 0.15 hour-1, 0.60 +/- 0.17, 0.40 +/- 0.02 mm and 290 +/- 35 micron2, respectively (mean +/- SD). The values of Kac, CT and CS increased markedly after freezing and decreased gradually with passage of time. The Kac and CT recovered to normal level by the 6th and 4th week after freezing, respectively. The positive significant correlation was found between the values of Kac and CT (r = 0.84, P less than 0.001), and between those of kac and CT (r = 0.58, P less than 0.001). On the contrary, no significant correlation was found between Kac and CS. The CS did not recover completely and remained about 32% larger than the values of the normal eyes even 30 weeks after freezing. Transmission and scanning electron microscopy of the endothelium revealed that the junctional complexes were reformed soon after the endothelial cells had made contact with each other, and the complexes became almost normal about 4 weeks after freezing. Normalization of the junctional complexes appeared to coincide with recovery of the Kac and CT.

Animals↗
PubMed1982

Pharmacokinetics of topical phenylephrine hydrochloride in the normal human eye.

Solutions of phenylephrine hydrochloride at concentrations of 1, 2 and 5% were instilled in one eye of 5 normal volunteers. Both eyes were photographed concurrently with an infrared film, and the pupil diameter was determined by an image analyzer using the developed film. The mydriatic effects were expressed by the difference in the pupil diameter of the experimental and the fellow control eyes determined concurrently. The mydriatic effects with these 3 solutions reached a peak about one hour and 15 minutes after instillation; the peak effects and the logarithm of the drug concentrations showed a linear relationship. An extrapolation of the relationship gave a least effective concentration of 0.07%. From the time course of the mydriatic effects, the pharmacokinetic coefficients were calculated using a computer. The average values were 0.24 +/- 0.08 hour for the time lag of the mydriatic response to appear, 1.31 +/- 0.19 hour-1 for the apparent elimination rate constant and 1.47 +/- 0.28 hour-1 for the apparent absorption rate constant.

Adult↗
PubMed1981

A mixed tumor of the omentum majus in an adult with discussion related to hepatoblastoma.

A mixed tumor of the omentum majus found at autopsy of a 28-year-old man with a huge liver tumor is described. The primary tumor was identified in the omentum majus by macroscopic, histologic and electron-=microscopic observations. The tumor was finally diagnosed as a mixed tumor which resembled a hepatoblastoma consisting of immature and embryonal types of epithelial component and of mesodermal tumor including hemangioma, osteoid and myxomatous tissues in the primary lesion. Widespread multiple metastases of the epithelial component mainly to the liver, and partially in the lungs, diaphragm and lymph nodes were noted. The levels of alpha-fetoprotein and carcinoembryonic antigen were within normal limits, but the epithelial component showed a resemblance to primitive hepatocytes. This tumor was discussed related to hepatoblastoma in an adult, with the features compatible with description of the same tumors reported previously in the literature.

Adult↗
PubMed1981

Mechanism of glycogenosome formation in axons of cadmium-induced neuropathy--ultrastructural and biochemical studies.

Numerous glycogenosomes were found in the axoplasm of the sciatic nerves in neuropathy induced by the long-term administration of cadmium to rats. The glycogen particles in the glycogenosomes were larger than those deposited in the intracytoplasmic matrix. Two forms of alpha-glucosidases, acid and neutral, were found in the sciatic nerve of normal rat. Cadmium ions inhibited the activity of the neutral alpha-glucosidase by a maximum of 24.0%, while acid alpha-glucosidase was not inhibited. Hg ions completely inhibited both forms of alpha-glucosidases; however, it has been reported that no glycogenosomes appear in Hg++-induced neuropathy. It was considered that the inhibitory effect of cadmium on the neutral alpha-glucosidase led to the enlargement of glycogen particles, and the large glycogen molecules were then engulfed in the lysosome system.

Animals↗
PubMed1980

Physicochemical and ultrastructural studies on glycogenosomes in newborn rat hepatocytes.

In the newborn rat liver, glycogenosomes appeared at about 6 hours after birth and gradually increased in number, despite high activity of lysosomal acid alpha-glucosidase. The glycogenosomes then disappeared completely with 2 days after birth. Glycogen extracted from fetal rat liver differed in molecular structure from that of adult rat liver. In measurements of optical rotatory dispersion (ORD) and circular dichroism (CD) absorption spectra, fetal-type glycogen showed the Cotton effect whereas the adult rat liver glycogen did not. The degree of branching and S-values of the two glycogens were also different. With the disappearance of glycogenosomes, fetal-type glycogen disappeared completely, and adult-type glycogen then appeared strongly. This was demonstrated in the intracytoplasmic matrix by electron microscopy. The present experiments indicate that glycogenosomes may be formed even under conditions of high acid alpha-glucosidase, and that autophagy of glycogen macromolecules in the lysosomal system is closely related to different structural features of fetal-type glycogen in the neonatal period.

Animals↗
PubMed1980

Ultrastructural and biochemical studies of glycogenosome formation in ascites hepatoma AH 13 cells.

Appreciable numbers of glycogenosomes were found in ascites hepatoma cells. They were lined by a single or sometimes double membranous structure exhibiting weakly positive acid phosphatase activity. Some were closely related to the Golgi apparatus, and a few showed wrapping of glycogen particles. The glycogen extracted from ascites hepatoma AH 13 cells was of two major types: a normal adult liver type, and a muscle type. Only the latter type gave the Cotton effect on measurement of the ORD and CD spectra. Small particulate, muscle type glycogen was observed in the glycogenosomes. Autophagy of normal adult liver type glycogen was never found. The engulfment of muscle type glycogen was accompanied by high acid and neutral alpha-glucosidase activity levels.

Acid Phosphatase↗
PubMed1978

An ultrastructural study of chronic cadmium chloride-induced neuropathy.

After the long-term exposure to cadmium chloride in drinking water, the Wistar rats developed peripheral polyneuropathy. The main lesion was of myelin degeneration. Ultrastructural examination of the roots and sciatic nerves revealed segmental demyelination beginning from the node of Ranvier. There was the active autophagocytosis of Schwann cells which contained a number of myelin remnants and dense bodies. There was, on the other hand, the evidence of remyelination with toxic damage, in which the thinner myelin sheaths and abnormal myelinations were observed with increase of Schwann cells containing rich ribosomes. Axoplasmic changes were minimal, but consisted of accumulation of glycogen particles which very often produced glycogenosomes in characteristic appearance with axoplasmal dysfunction.

Animals↗