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T Tsvetkova

Publications and source records attributed to T Tsvetkova.

At least 19 recordsLinked to original sources
PubMed2004

[Effect of low-dose oral contraceptive regulon-Gedeon Richter on some hemostatic variables].

The authors have investigated the changes of some haemostatic variables at 33 women, who used low-dosed oral contraceptive REGULON--Gedeon Richter in the duration of six months. Statistically significant decrease is found in the activity of Protein S and Protein C, but plasminogen level is increased. The levels of the other variables remained in referential ranges. The use of REGULON doesn't influence the coagulation system.

Adolescent↗
PubMed2001

Milrinone versus dobutamine in heart failure subjects treated chronically with carvedilol.

OBJECTIVE: To compare the efficacy of milrinone and dobutamine in patients chronically treated with carvedilol. BACKGROUND: Milrinone and dobutamine are used to manage decompensated heart failure, but their efficacy in patients on beta-blocker therapy was unknown. METHODS: Twenty patients with decompensated heart failure were prospectively enrolled. Inotropic responses to milrinone (12.5, 25 or 50 microg/kg bolus infusions) or dobutamine (5, 10, 15 or 20 microg/kg/min infusions) were evaluated by right-heart catheterization. RESULTS: Milrinone increased cardiac index (2.0-2.6 l/min/m2, P=0.0001) without significantly altering heart rate (70-75 bpm, P=0.19). Milrinone decreased mean pulmonary artery pressure (36-29 mm Hg, P=0.0001), pulmonary capillary wedge pressure (24-18 mm Hg, P=0.0001) and mean arterial blood pressure (78-75 mm Hg, P=0.0002). Left ventricular stroke volume index increased in the milrinone group (31-35 ml/beat/m2, P=0.0001). Dobutamine produced an increase in cardiac index (2.4-3.3 l/min/m2, P=0.0001) only at doses that are not typically used to treat heart failure (15-20 microg/kg/min). At these doses, dobutamine increased heart rate (68-82 bpm, P=0.008), mean systemic pressure (90-117 mm Hg, P=0.0001) and mean pulmonary artery pressure (21-30 mm Hg, P=0.001). Dobutamine did not alter left ventricular stroke volume index or pulmonary capillary wedge pressure. CONCLUSIONS: Dobutamine and milrinone have different hemodynamic effects in patients treated chronically with carvedilol. These differences should be considered when selecting inotropic therapy for decompensated heart failure.

Adrenergic beta-Agonists↗
PubMed2001

[Main lymphocyte populations and their subpopulations in patients with acute pancreatitis studied in the course of disease].

The aim of the study was to establish the changes in the main lymphocyte populations and their subpopulations in peripheral blood of patients with acute pancreatitis. Seventy-one patients were studied in the course of disease. They were classified on the basis of severity of pancreatitis--mild and severe form. Percentage and absolute numbers of CD19+ (B lymphocytes), CD2+ (total T cells), CD3+ (immunocompetent T cells), CD3+CD4+ (helper T cells), CD3+CD8+ (suppressor/cytotoxic lymphocytes), CD4+CD29+ and CD4+CD45-RA+ (helper/inducer and suppressor/inducer subpopulations of helper lymphocytes). Immunophenotype analysis was performed using a flowcytometer Epics XL-MCL, Coulter, USA and monoclonal antibodies by the same company. In the early stage of disease the fall of total CD2+, immunocompetent CD3+ T lymphocytes and their subpopulations (more expressed in the severe form) is an indicator of determining the severity of pancreatitis. These results along with the elevated CD4+CD29+ subpopulation are a sign of both activation of these links in the local immune reaction and development of secondary infectious complications. The observed fall in CD4+CD45-RA+ suggests increased risk of development of autoimmune process. The recovery of most of the studied parameter after day 3 shows unimpaired host compensatory capabilities for both forms of disease.

Adolescent↗
PubMed1998

A novel ETA-AAS background reduction method for determination of lead and cadmium in urine.

Urinary lead and cadmium level were determined in double diluted urine samples using two modifiers: 1% HNO3 (own modification) and 1.6% NH4H2PO4 that is generally used for matrix modification. The background signal is reduced in the samples diluted with 1% HNO3 because of the modifying effect of nitric acid. Lower variation coefficients were obtained in a run of 10 determinations of each of the samples processed by our method which is an advantageous feature compared with the method using 1.6% NH4H2PO4 as a modifier. Recovery is between 95 and 105% for both methods. The analytical performance, flexible pre-instrumental preparation and minimized risk of sample contamination make the use of 1% HNO3 modifier a reliable method in the routine practice of determination of the urinary Pb and Cd level.

Cadmium↗
PubMed1997

Transformation and average as handy tools for pressure measurement data analysis.

INTRODUCTION:: Pressure measurement data underneath[Table: see text] the foot represent usually a large number of data files which is equal to double product of the number of the subjects in study and of the number of the trials for each foot. Besides, 'normal' parameters are necessary to compare the results for the controls and for the subjects in study. Statistical parameters (mean values and standard deviations) are not very convenient when it is required to establish cartographical and geometrical parameters or peak pressures, maximum forces, contact areas, contact time and others for foot typing or as a standard. Sometimes it is reasonable to obtain average pressure data as a base for further data analysis. But taking into account that pressure distribution pattern may differ by its length, width and angle of the inclination of the foot axis to the horizontal it was necessary to solve a problem of transformation of any pressure picture to the given length and width (for vertical location of the foot axis). METHODS:: The transformed coordinates X(*) and Y(*) are defined from the following system of equations: X = Y(*) x width/width(*) x sina + X(*) x length/length(*) x cosa + X(o) Y = Y(*) x width/width(*) x cosa - X(*) x length/length(*) x sina + Y(o) where X(o), Y(o) -- the coordinates of the origin; width, length, a -- parameters of the initial pressure picture; width(*), length(*) -- parameters of the transformed pressure picture. X, Y -- are real values but the values of pressure are defined only in the integer coordinates. One of the main parts of average is a reduction of the time process to any number of frames. If N(max) is a maximum number of frames in one of the data files than all other data files must be transformed with accordance to the following formulas: for j-th frame the value of pressure for sensor with coordinates x, y is: P(*1)(xy) (j) = P(1)(xy) (i) x ki + P(1)(xy) (i+1) x k(i+1), where P(1)(xy) (i) -- pressure for sensor with coordinates x, y of the 1-th initial time process; j = 0, ellipsis, N(max) -1; j-th frame corresponds to i-th frame in the initial time process as i = integer(j) x (N -1)/(N(max) -1), N -- a number of frames in initial time process; coefficients k(i) and k(i+1) are equal to k(i) = j x (N -1)/(N(max) -1) -- I, k(i+1) = 1 - ki. Then the values of pressure are averaged: P(*)(xy) (j) = 1 / L x S P(1)(xy) (j), where L is a number of data files. Corresponding software was developed. RESULTS AND DISCUSSION:: 38 files of pressure measurement data of the teenagers from military school were chosen for transformation and average. Statistical errors of transformation and average were calculated for each data file (for peak pressure, force and area) and for all parameters from two software packages: Novel-win v.95.121 and Novel-ortho v.95.121. The maximum errors are for peak pressures (about 7%-10%) and contact areas (about 6%). CONCLUSION:: Transformation and average programs can be successfully used for data analysis additionally to traditional statistical software: groupmask evaluation program.

Journal Article↗
PubMed1997

Velocity of the cop as a reliable parameter for early diagnosis of coxarthrosis.

INTRODUCTION:: The first symptoms of the degenerative disease of hip joint appear very early when people are about 14 years old. But clinical and X-ray characters manifest themselves insufficiently: temporary slight pains in hip joints on loading, insignificant painfulness along posterior surface of the joint during palpation, slight lameness on the diseased side. The purpose of this study was to try to involve gait analysis in the clinical research of the teenagers with coxarthrosis and to define the parameters which may be used for early diagnosis of coxarthrosis. METHODS:: 38 male military school teen-agers which have no history in the foot disorders and 20 male and 9 female with coxarthrosis were used for study. Plantar pressure distribution measurements were performed with the mini-emed system (Novel, Munich, Germany). Five dynamic records of each foot were made on the pressure platform (28 x 60 sensors, resolution -- 3 sensors/cm(2), sampling frequency -- 16 Hz). Three trials of gait were accomplished by each subject with inserted insoles (99 sensors in each insole) at the distance about 10 m. Novel-win v.95.121 and Novel-ortho v.95.121 packages were used for data analysis. RESULTS:: For the teenagers with coxarthrosis the loading of heel area is significantly less (< = 15 N/cm(2)), maximum force is generally less. Contact time (in percent of roll-over-process) in the forefoot and toes is increased and is equal to 100% very often. The most interesting results are obtained for the curves of the velocity of the centres of pressure versus time. For the controls this curve has one or two maxima (during 50%-70% of roll-over-process when heel, midfoot and sometimes forefoot are loaded), then a short period of insignificant changes of the velocity (up to 88%-94% of roll-over-process when only forefoot and toes are loaded) and last a very short period of its increase (toe-off phase). For the most part of the teenagers with coxarthrosis this curve seems to be monotonous, oscillating near its mean value line. DISCUSSION:: These disturbances may be explained by valgus deviation of the neck of femur and insufficient function of the muscles of buttocks as a consequence of its lever web decrease. The muscle forces (particularly abducted muscles) are decreased (about 10%-15%). These data are consistent with previous reported findings. CONCLUSION:: To compensate for the painfulness in the diseased hip joint the teenagers load rearfoot and forefoot less than in the 'normal' foot, increasing contact time in the forefoot. As a consequence of disturbances in hip joint a pathway of the centre of pressure is longer than in 'norm' and its velocity changes oscillating near the mean value when for 'normal' teenagers three definite intervals of its change are viewed. Therefore the first symptoms of displastic coxarthrosis of teenagers may be revealed from plantar pressure measurements by analysing the character of time dependence the velocity of the centres of pressure and loading of different areas of the foot (force and contact time). Additionally, functional state of hip joint muscles, X-ray and clinical symptoms, heat radiation and laser biophotometry data give an opportunity to define the character of locomotor function disorders.

Journal Article↗
PubMed1997

Early diagnosis of coxarthrosis (in-shoe plantar pressure measurements).

INTRODUCTION:: Plantar pressure measurement during walking adds dynamic functional data to evaluation of gait disorders of teenagers with coxarthrosis. The duration of the complete gait cycle, its phases (stance and swing phase for right and left feet, double support) and phases of roll-over process are found useful in many clinical applications. METHODS:: In-shoe measurements were completed with Pedar system (99 element capacitive insoles from Novel, Munich, Germany) for 38 teenagers from military school (controls) and 29 teenagers with coxarthrosis. The data from 3 - 5 trials for each subject were collected. The distance was about 10 m, no requirements to the speed and step length were established. Special software was developed. RESULTS:: Time parameters for the complete gait cycle, its phases, phases of roll-over-process were calculated both for the controls and the teenagers with coxarthrosis It was found that stance phase and heel contact phase are decreased for the teenagers with coxarthrosis. DISCUSSION:: Decrease of stance phase, insufficient loading of the heel and decrease of heel contact time are a result of attempts to compensate the painfulness in the hip joint during walking. CONCLUSION:: Time parameters of the complete gait cycle and its phases may be useful in early diagnosis of coxarthrosis and evaluation of efficiency of the treatment.

Journal Article↗
PubMed1997

Evaluation of an ion-selective electrolyte analyzer: Microlyte 6.

Microlyte 6 (Kone, Finland) is an ion-selective analyzer designed to measure simultaneously the concentration of six important electrolyte parameters--potassium, sodium, chloride, ionized calcium, ionized magnesium and pH in whole blood, serum and plasma. Two values are obtained in analyzing the ionized fractions of magnesium and calcium--one at the actual pH and another at a recalculated measurement for pH = 7.4. Direct determination of ionized calcium and ionized magnesium simultaneously with that of the other electrolytes is of great clinical significance. It is only recently that ion-selective analysis of ionized magnesium has been proposed. The analytical reliability of the results and the operational characteristics of the Microlyte 6 ion-selective analyzer were evaluated for approximately one year. The coefficient of variation of the results in the reference and pathological range was 0.49%-2.23%, and 0.68%-4.42% for the within-run and between-run series, respectively. The inaccuracy of the results expressed by d% was from -4.23% to +4.06%. The comparative evaluation of the results for potassium, sodium, chloride, and ionized calcium between Microlyte-6 and the clinical chemistry analyzer Dynamic (Kone) showed a high correlation (correlation coefficient in the range 0.9868-0.9970). The correlation between the results for the ionized fraction and those obtained for total magnesium was consistent with that generally given in the literature.

Chemistry, Clinical↗
PubMed1992

Aspects of granulocyte function in workers professionally exposed to pesticides.

Granulocyte function in 92 workers from a chemical plant for the production of pesticides was tested by means of the nitro-blue tetrazolium test (spontaneous and stimulated) and the phagocytosis test. Two opposite types of change were identified, namely increased and reduced functional activity. No reliable correlation between the studied parameters and length of service was found. In comparison with the routine hematological methods, the identified functional changes in polymorphonuclear neutrophil granulocytes serve as an early indicator of an impact on the leukocytes. The applied methods are accessible and may be used as an objective means of studying the dynamics of the unfavorable effect of zineb and of identifying groups at increased toxicological risk.

Adult↗
PubMed1990

[The antiphospholipid syndrome in patients with systemic lupus erythematosus and in their closest blood relatives (a preliminary report)].

The description of a phospholipid syndrome in patients with systemic lupus erythematodes (SLE) filled up a gap in defining an "atypical" subgroup--quite often negative to DNA antibodies but suffering from arterial thromboses, thrombophlebitis, aseptic bone necroses, and pulmonary hypertension. 54 patients with SLE and 32 of their closet blood relatives were examined for lupus anticoagulation factor, anticardiolipin antibodies and Wassermann test. The results were interpreted in relation to the forms of the disease and its clinical manifestations. Clinically healthy relatives positive for circulating lupus anticoagulation factor or antiphospholipid antibodies are under close control.

Autoantibodies↗
PubMed1990

[Alkaline phosphatase in the leukocytes of pregnant women with pre-eclampsia].

The authors studied 74 pregnant women with preeclampsia presenting various degrees of gravity. The activity of alkaline phosphatase was determined cytochemically in the leucocytes. It was established that the activity of the enzyme was unchanged in mild preeclampsia and diminished progressively with advancement of the pathological process, compared with the respective values of normally progressing pregnancy at the same gestational period. A cytochemical criterium is proposed for differentiating the mild form of preeclampsia form the moderate form of preeclampsia.

Alkaline Phosphatase↗