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Biomedical subjects

T Turnbull

Publications and source records attributed to T Turnbull.

14 recordsLinked to original sources
PubMed1995

Comparison of the T helper cell response induced by respiratory syncytial virus and its fusion protein in BALB/c mice.

Specific proliferative T-cell responses were induced in the lymph node cells (LNC) of mice immunised with a sucrose density gradient purified preparation of respiratory syncytial (RS) virus or an immunoaffinity purified preparation of the F glycoprotein. Inhibition studies and flow cytometric analysis showed that the responding cell population were CD4+ T cells. The cytokines produced by virus-specific and F-specific cells were assessed using the CTLL cell line. Peak quantities of cytokine were consistently detected in the supernatants of stimulated cultures 24 h prior to maximum proliferation. The proportion of IL-2 released was determined by blocking IL-2 activity with an anti-IL-2 monoclonal antibody. In cultures of RS virus primed LNC challenged with whole virus there was a switch of cytokine production from 70% IL-2 at day 3 to 80% IL-4 by 6 days of culture. In contrast, LNC cultures from mice immunised with F protein secreted 75-100% IL-2 throughout the culture period. These data suggest that after 6 days of challenge with viral antigen, the RS virus-primed LNC response consists of T helper cells which are predominantly of the Th2 subset, secreting IL-4, whilst F protein-primed LNC secrete large quantities of IL-2 and can therefore be classified as predominantly of the Th1 subset.

Animals↗
PubMed1991

Recognition of respiratory syncytial (RS) virus proteins by human and BALB/C CD4+ lymphocytes.

Lymphocyte proliferation assays were used to determine the ability of human and BALB/c T-lymphocytes to recognise and respond to in vitro challenge with purified preparations of four respiratory syncytial (RS) virus proteins. Human peripheral blood lymphocytes (PBLs) collected from adult donors as well as primed BALB/c mouse splenocytes each responded specifically to challenge with intact RS virus and preparations of the fusion (F), attachment (G), 23 kilodalton (23K), and 34K phospho- (P) proteins of the virus. F protein was recognised most frequently by human PBLs, and elicited higher levels of response than equivalent concentrations of the other protein preparations examined. The human PBL proliferative responses elicited by in vitro challenge with intact virus antigen as well as with each of the four protein preparations were found to be confined to the CD4+ T-helper (Th) sub-population of lymphocytes. However, proliferative responses to intact virus and F protein were found to be accompanied by only modest and inconsistent production of Interleukin-2 (IL-2). Finally, no evidence was obtained to indicate that any of the challenge antigens employed in this study were intrinsically mitogenic, as neither naive human cord blood lymphocytes, nor un-primed BALB/c mouse splenocytes proliferated when challenged with intact RS virus or with F, G, 23K, or P protein preparations.

Adult↗
PubMed1990

Cardiopulmonary abnormalities after smoking cocaine.

We recently treated a young man who had chest pain after smoking "freebase" cocaine. Pneumomediastinum was diagnosed, and the electrocardiographic changes that developed were characteristic of coronary artery spasm. Although pneumomediastinum has been linked to "freebasing," we believe electrocardiographic abnormalities in this situation have not been previously reported.

Adult↗
PubMed1989

Complications and protocol considerations in carbon monoxide-poisoned patients who require hyperbaric oxygen therapy: report from a ten-year experience.

We conducted a study to determine the type, incidence, and timing of complications that occur in patients who have a carbon monoxide (CO) exposure serious enough to require hyperbaric oxygen therapy (HBOT). Complication data were retrospectively collected from a ten-year period for 297 consecutive CO-poisoned emergency department patients who received HBOT. HBOT was indicated for 41% of the patients because of an elevated carboxyhemoglobin (COHb) level alone. Central nervous system dysfunction, including loss of consciousness, and/or cardiovascular dysfunction, was the criteria for HBOT in 59% of patients, regardless of their COHb level. The mean peak COHb level was 38 mg%, with 88% of patients having a peak COHb level greater than 25 mg%. The mortality rate was 6% in this case series. Cardiac arrest occurred in 8% of patients; all experienced their first arrest prior to HBOT. The 3% of patients who sustained an isolated respiratory arrest and those who had a myocardial infarction did so prior to HBOT. Several complications, however, occurred for the first time or as a recurrent event during HBOT. These included emesis (6%), seizures (5%), agitation requiring restraints or sedation (2%), cardiac dysrhythmias or arrests (2%), and arterial hypotension (2%). No patient's level of consciousness deteriorated subsequent to the initial resuscitation except for those who later had a generalized seizure. The most significant complication attributable to HBOT was tension pneumothorax, noted in three patients (1%).(ABSTRACT TRUNCATED AT 250 WORDS)

Carbon Monoxide Poisoning↗
PubMed1986

A comparison of Keller's arthroplasty and distal metatarsal osteotomy in the treatment of adult hallux valgus.

A prospective trial is reported which compares distal osteotomy of the first metatarsal with Keller's arthroplasty in the treatment of adult hallux valgus. A total of 33 patients attended for review at least three years after operation. Symptomatic improvement, as assessed by patient satisfaction, pain relief, cosmetic improvement and restoration of function, was similar in the two groups. Objective measurement showed that the range of movement of the metatarsophalangeal joint was better maintained after osteotomy, as was the relationship of the sesamoid bones to the head of the first metatarsal. Correction of the valgus deformity also was significantly better in the patients who underwent osteotomy and in these patients the first intermetatarsal angle was reduced to within normal limits. There was no evidence that initial degenerative changes or subluxation at the metatarsophalangeal joint compromised a successful result from osteotomy.

Adult↗
PubMed1981

Fixer recycling combined with silver recovery.

A trial over nine months has been described, of equipment combining fixer-recirculation with electrolytic silver recovery. The equipment has performed satisfactorily and a reduction of 50% in fixer consumption has been achieved without encountering difficulty. An increase in silver yield has resulted, although not yet accurately quantified. The equipment is neat and compact (25 in X 16 in X 29 in high) and will fit under a darkroom bench. It was fitted to a busy processor, handling among other things, the accident and emergency workload. It has proved adequate to deal with a heavy workload and further installations in the district are planned.

Silver↗