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T V Fischer

Publications and source records attributed to T V Fischer.

15 recordsLinked to original sources
PubMed1992

ATLAS-plus: multimedia instruction in embryology, gross anatomy, and histology.

ATLAS-plus [Advanced Tools for Learning Anatomical Structure] is a multimedia program used to assist in the teaching of anatomy at the University of Michigan Medical School. ATLAS-plus contains three courses: Histology, Embryology, and Gross Anatomy. In addition to the three courses, a glossary containing terms from the three courses is available. All three courses and the glossary are accessible in the ATLAS-plus environment. The ATLAS-plus environment provides a consistent set of tools and options so that the user can navigate easily and intelligently in and between the various courses and modules in the ATLAS-plus world. The program is a collaboration between anatomy and cell biology faculty, medical students, graphic artists, systems analysts, and instructional designers.

Anatomy↗
PubMed1979

Adult rat lung in organ culture: maintenance of histotypic structure and ability to synthesize phospholipid.

The maintenance of adult rat lung explants in organ culture was assessed both morphologically and biochemically. A comparison of several culture media indicated that Ham's F12K plus 1.0 microM dexamethasone, which maintained the explants for 14 days, was superior. The ability of the explants to synthesize dipalmitoylphosphatidylcholine increased with the length of cultivation to values greater than the noncultivated controls. The DNA content remained constant for 7 days, and a relatively normal structural relationship between type I and type II pneumocytes was maintained. Explants cultivated in Ham's F12K without dexamethasone did not maintain a histotypic morphology; the type II pneumocytes appeared to proliferate and the ability to synthesize phosphatidylcholine decreased.

Animals↗
PubMed1978

Effect of vitamin A and methylprednisolone on canine prostate in organ culture.

Organ explants from the canine prostate with and without methylprednisolone pretreatment were cultivated for ten days in Trowell's T-8 medium or medium supplemented with testosterone and/or vitamin A. Upon termination of the experiments, explants were fixed and examined histologically. All glands from the most central section of each explant were graded according to epithelial type, and from these grades the proportion of acini with maintained columnar cells was calculated for each explant. Stromal cellular maintenance was also estimated. While a small proportion of explants from the vitamin-treated and methylprednisolone-pretreated groups showed epithelial maintenance, a combination of these treatments significantly increased such maintenance. Stromal maintenance was enhanced with methylprednisolone pretreatment but not by vitamin A. These results are in accord with the hypothesis that methylprednisolone acts to stabilize the lysosomal membrane, thus protecting tissue against the effects of ischemic shock. In protected explants vitamin A is able to maintain a columnar glandular epithelium. In a subsequent experiment a series of linoleic acid dosages was tested in the presence or absence of vitamin A. In neither case was this fatty acid of value in improving epithelial or stromal maintenance.

Animals↗
PubMed1978

Organ culture of canine prostate.

The simple life support system of organ culture contrasts sharply with the life support system in vivo. Organ culture, a technique for characterizing the effect of various substances on an organ, has potential clinical usefulness in many areas. In the present case explants of canine prostate are minimally maintained when cultivated in a medium enriched with testosterone and insulin. Pretreatment of the dog with methylprednisolone or the addition of vitamin A markedly improves the maintenance of normal structure. Methylprednisolone pretreatment and the addition of vitamin A had a significant synergistic effect. The clinical significance and implications of the findings are presented. We believe these preliminary experiments with organ culture can serve as a basis for deciphering some of the complex functions or an organ.

Animals↗
PubMed1976

Effect of testosterone on long-term organ cultures of canine prostate.

Organ cultures of rodent and human prostate glands have shown marked differences in their morphological response to testosterone. In this study, explants from 19 canine prostate glands were cultivated for a minimum of 9 days in Trowell's T-8 medium. Groups of explants were exposed to media containing from 0.05 to 100 mum testosterone. While the higher testosterone levels (50 and 100 mum) markedly decreased explant viability, explants cultivated at lower levels (0.05 to 5 mum) appeared similar to control explants in testosterone-free Trowell's T-8 medium. Atmospheric mixtures containing either 95% or 50% oxygen were equally effective. Shortly after the cultures were initiated, large amounts of secretory product were liberated into the lumen. After 9 or more days in vitro, glandular epithelium appeared cuboidal and never revealed the acid phosphatase-rich secretory granules seen in the preculture control. However, the epithelium exhibited an increase in alkaline phosphatase and lipid content following cultivation.

Alkaline Phosphatase↗
PubMed1975

Effect of gonadotropins on ovulation and ovarian histology in the immature Mongolian gerbil.

The present study is an analysis of the effects of superovulatory doses of gonadotropins on the rate and time of ovulation and ovarian histology in immature gerbils. Groups were treated with various combinations of pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (HCG). The maximum superovulatory response followed treatment with 10 IU PMSG and 20 IU HCG. High dosages of PMSG inhibited superovulation, as did some combinations involving the highest dose of HCG. Entrapment of ova within corpora lutes was common in groups receiving high doses of either gonadotropin. Luteal regression, appearing by day 3, occurred often in groups receiving high doses of HCG. A dose of 10 IU PMSG and 5 IU HCG resulted in both a near maximal superovulatory response and the least abnormal ovarian alteration.

Animals↗