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Biomedical subjects

T V Mamontova

Publications and source records attributed to T V Mamontova.

At least 19 recordsLinked to original sources
PubMed1992

[The effect of the bronchoalveolar fluid of mice infected with the influenza virus on hematopoietic bone marrow precursors].

The influence of bronchoalveolar washing fluid (BAWF), as well as BAWF cells, obtained from mice infected with influenza virus, on the formation of exogenic spleen colony-forming units (CFUs) of lethally irradiated syngeneic recipients was studied. BAWF and BAWF cells of intact syngeneic mice stimulated the growth of CFUs. BAWF of mice infected with nonpathogenic strain A/PR/8/34 lost its capacity for stimulating the growth of colonies, and BAWF cells greatly suppressed colony formation in the spleen of recipients. The participation of interferon, colony-stimulating factor and the virus itself in the process of the modulation of colony formation is discussed.

5'-Nucleotidase↗
PubMed1988

[Effect of modulation of the production of interferon by L-929 cells treated with theophylline].

The specific inhibitor of cAMP phosphodiesterase theophylline has been shown to evoke in L929 cells 2.3-fold induction of 2-5A-synthetase activity and 3.5-fold superinduction of the same enzyme activity while acting in combination with actinomycin D. It has been shown also that temporal coincidence of 2-5A-synthetase induction with the active period of interferon production resulted in 8-16 times decrease in the level of interferon production. The result was supported by the experiments of superinduced cells (containing the high stable level of 2-5A-synthetase) fusion with monolayer of poly(I).poly(C)-induced L929 cells (taken at the start of interferon production). In this case the production of interferon was dramatically decreased in comparison with the control. Possible role of 2-5A-synthetase in regulation of interferon production is discussed.

2',5'-Oligoadenylate Synthetase↗
PubMed1987

The influence of high temperature on human cells in culture, their sensitivity to viruses and their interferon-producing capacity.

The influence of extremely high temperatures on the course of virus infection in vitro was studied. It has been shown that exposure of human cells (continuous cell line of embryonal skin-muscle tissue, donor blood leukocytes) to 43 degrees C leads to a significant increase in their sensitivity to cytopathic action of the viruses tested. Noteworthy, the reproduction of these viruses under tested conditions did not differ from their reproduction in control cells. A dramatic inhibitory effect of high temperatures on the interferon-producing capacity of cells was observed.

Cell Line↗
PubMed1980

Stimulation by calcium chloride of virus-induced interferon formation by blood, haemopoietic organ and continuous human cells.

Calcium chloride stimulated virus-induced production of leukocyte interferon by human and animal blood and haemopoietic organ cells. CaCl2 treatment of surviving cells (leukocytes, human and mouse bone marrow) and Namalva cells was the most effective when carried out simultaneously with adsorption of the virus-inducer or when CaCl2 pretreatment was combines with its subsequent addition together with the virus-inducer. Optimal CaCl2 concentrations were 5 mM for human bone marrow cells and 10 mM for human leukocytes and mouse bone marrow cells. CaCl2 treatment was equivalent to priming in case of interferon induction in human leukocytes and, as distinct from priming, it considerably increased virus-induced interferon production by Namalva cells.

Animals↗
PubMed1977

[Antiviral activity of preparations of RNA isolated from cells treated with interferon (messenger RNA for an antiviral protein?)].

Chick embryo cultures treated with interferon yielded a biologically active RNA which, upon inoculation into chick embryo cells, created an antiviral condition in them. The level of vesicular stomatitis virus reproduction in such cells was 2-30% of that observed in the cells treated with control RNA. The maximum activity of the experimental RNA was seen 3 hours after the treatment with interferon.

Animals↗
PubMedDate not supplied

[Isolation and characteristics of anti-adenovirus monoclonal antibodies in immunoenzyme and immunofluorescent reactions].

New monoclonal antibodies (MAbs) to adenovirus hexon, highly active in ELISA and immunofluorescent analysis, were prepared. According to competitive ELISA, new MAbs differed in their blocking activity and were directed to 2 different hexon epitopes. MAb 3H8 did not modify antigen binding of the rest MAbs labeled with peroxidase (PAb x Pox), and none of unlabeled MAbs suppressed the reaction of MAb x Pox 3H8. MAbs 1E8 7F1, 1E11, and 3B1 reacted with each other but differed by the spectrum and level of competitive inhibition, which indicated that they were directed to different epitopes of adenovirus hexon. Comparison of the specific activity of MAbs 7F1 and 1E8 in direct immunofluorescent detection of adenovirus antigens in infected cell cultures and clinical materials from patients showed a good coincidence (90-97%) of the results with the IMAGEN Adenovirus test (Dako) and with polyclonal FITC conjugates to adenovirus hexon.

Adenoviridae↗
PubMedDate not supplied

[Synthesis of virus-specific RNA under conditions of homologous and heterologous interference by Newcastle disease virus].

Homologous and heterologous interference by Newcastle disease virus (NDV) was manifested in a marked inhibition of virus-specific RNA synthesis of both the interacting viruses. Inactivation of the interfering NDV by irradiation reduced its capacity to inhibit the synthesis of virus-specific RNA of the homologous virus, leaving its effect on the synthesis of RNA of the heterologous virus unchanged. In contrast, treatment of cell cultures with heparin leading to reduced interferon production eliminated the heterologous interference but did not affect the homologous interference.

Animals↗
PubMedDate not supplied

[Nonspecific non-reactivity in mice induced by joint administration of Newcastle disease virus and cyclophosphamide].

The immunomodulating effect of Newcastle disease virus (NDV) was investigated in vitro and in vivo in mice. NDV was shown to induce a mitogenic effect in splenocytes in vitro. Combined injections of NDV and CP resulted in nonspecific suppression of immunoreactivity in mice. The antibody production and development of delayed type hypersensitivity to sheep erythrocytes were markedly reduced. Injections of NDV alone slightly increased the reactions. The NDV + CP injections led also to a reduction of immune response to thymus-independent antigen, LPS. Thus, the combined injections of NDV and CP led to nonspecific suppression of T and B cell immunity in mice. The mechanisms of this form of anergy require further study.

Animals↗
PubMedDate not supplied

[Producer cells of human alpha and gamma interferons].

The cells responsible for production of human alpha and gamma interferons with proper inducers were studied using methods for fractionation of human blood mononuclear cells. The results indicated cooperation and mutual regulation by cells of interferon production. Monocytes were shown to play the leading role in the synthesis of acid-stable alpha interferon, whereas accessory cells were required for gamma interferon synthesis by T lymphocytes. Acid-labile alpha interferon was produced predominantly by B cells with accessory participation of T lymphocytes.

B-Lymphocytes↗
PubMedDate not supplied

[Modulation of the induction of interferon formation by calcium chloride].

The results of the study confirm earlier reports on the stimulating effect of calcium ions on production of interferon induced by poly(I) X poly(C) and on translation of messenger RNA for interferon. Neutralization of the inhibiting activity of interferon production suppressor present in the cytoplasm of normal cells has first been demonstrated which broadens our concepts on the mechanism of action of calcium ions.

Animals↗