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T V Shankey

Publications and source records attributed to T V Shankey.

28 records · Page 2Linked to original sources
PubMed1989

Flow cytometric analysis of human lymphocytes using affinity-purified antibody to T cell receptor beta synthetic J region peptide.

The purpose of this study was to use affinity-purified polyclonal antibodies produced against a synthetic peptide corresponding to the joining (J) region of a human T cell receptor beta chain to characterize antigen receptor expression on subpopulations of human lymphocytes. The synthetic peptide used was ANYGYTFGSGTRLTVV, corresponding to the J segment of the human beta-chain gene YT35. Biochemical characterization has previously demonstrated binding of anti-J beta peptide antibodies to the alpha/beta heterodimer and to certain immunoglobulin light chains. Flow cytometric analysis of normal human peripheral blood lymphocytes performed here, using affinity-purified antibodies to the J beta peptide, showed expression of the epitope on 50-60% of CD20 (B1)-positive B lymphocytes, and on 40-50% of CD8-positive T lymphocytes. Only background levels were observed on CD4-positive T cells.

Antigens, Differentiation, T-Lymphocyte↗
PubMed1983

Cryptosporidiosis in a patient with hemophilia, common variable hypogammaglobulinemia, and the acquired immunodeficiency syndrome.

A 36-year-old man had chronic, debilitating diarrhea due to cryptosporidiosis. This patient had longstanding common variable hypogammaglobulinemia and recurrent bacterial infections. Immunologic evaluation after discovery of Cryptosporidium showed lymphopenia with persistently reduced numbers of helper/inducer cells (OKT-4), variable numbers of suppressor/cytotoxic cells (OKT-8), OKT-4/OKT-8 ratio of 0.09, and increased levels of serum alpha-interferon, all of which describe the acquired immunodeficiency syndrome. Oocysts of Cryptosporidium were found in feces from the patient's cat, thus identifying a possible source of his infection. The patient had disseminated candidiasis, cytomegalovirus pneumonia, and cryptosporidiosis when he died.

Adult↗
PubMed1981

Proliferation, differentiation, and cytogenetics of chronic leukemic B lymphocytes cultured with mitomycin-treated normal cells.

Lymphocytes from 6 patients with chronic lymphocytic leukemia of the B-cell variety (B-CLL) were cultured with equal numbers of mitomycin-treated mononuclear cells from normal blood. When stimulated with pokeweed mitogen (PWM), phytohemagglutinin (PHA), or the tumor-promoting agent, phorbol tetradecanoyl-acetate (TPA), the CLL cells proliferated actively by day 3 or 4 of culture, and in four cases, differentiated to significant numbers of immunoglobulin-containing cells. Chromosome studies on the proliferating lymphocytes demonstrated a cytogenetically abnormal clone in three patients, including two with a 14q+ marker chromosome and two with a translocation involving the short arm of chromosome 9. One patient had a translocation from 22q to 14q, producing a Philadelphia chromosome as well as the 14q+ marker. The results indicate that the neoplastic lymphocytes of B-CLL may proliferate and differentiate when appropriately stimulated in vitro, and that chromosomally abnormal clones are not uncommon. With several techniques now available for successful short-term culture of B-CLL lymphocytes, there is opportunity for better understanding of the cellular alterations in this disease.

B-Lymphocytes↗
PubMed1981

Kinetics of mitogen-induced proliferation and differentiation of human peripheral blood B lymphocytes.

Using a culture system that includes a B-enriched fraction of peripheral blood lymphocytes (PBL) mixed with non-proliferating "filler" cells (mitomycin-treated PBL or T cells), the kinetics of human B cell proliferation and differentiation into cells containing cytoplasmic immunoglobulin (cIg) have studied. Following stimulation with phytohaemagglutinin (PHA) or pokeweed mitogen (PWM), proliferative responses of enriched B cells were comparable to those for unseparated PBL, with a peak at 3 to 5 days and a steep decline thereafter; cIg-positive cells increased with both mitogens from day 3 until 7. Little proliferation or differentiation occurred in B cell cultures lacking T "filler" cells. The results indicate that human B cells can respond polyclonally to PHA in vitro, as well as to PWM, if non-proliferating T cell "help" is present. Further, these proliferative responses, but not subsequent differentiation, were inhibited by PHA-stimulated blasts that were treated with mitomycin and added on day 3 of culture.

Adult↗
PubMedDate not supplied

The acquired immune deficiency syndrome (AIDS) in hemophiliacs: a hypothesis.

Healthy hemophiliacs receiving antihemophilic (AHF) concentrates have decreased T-helper/suppressor (H/S) ratios, similar to the changes observed in healthy homosexuals considered at risk for the acquired immune deficiency syndrome (AIDS). We present a hypothesis which focuses on the role of natural killer (NK) cells and elevated serum levels of alpha interferon (IFN alpha) in hemophiliacs with AIDS. This hypothesis suggests that alloantigens in AHF concentrates provide an important inducing event which compromises the immune system of the hemophilia patient and enhances his susceptibility to infection by a transmissible agent. It provides an explanation for the predominance of NK cells with T-cell markers, impaired NK cell function, elevated serum IFN alpha levels, and the deficit in T-helper/inducer cells in hemophilic patients with AIDS. Furthermore, it could account for the T-cell subset imbalance in relation to total AHF units transfused, and to the development of an inverted H/S ratio which is a constant feature of AIDS.

Acquired Immunodeficiency Syndrome↗