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T Woźniakowska-Gesicka

Publications and source records attributed to T Woźniakowska-Gesicka.

13 recordsLinked to original sources
PubMed2001

[Influence of interferon-alpha therapy on the count and function of T lymphocytes in children with chronic hepatitis C].

This study aims to assess number and function of T lymphocytes in children with chronic hepatitis persistent C. The study included 60 children with chronic hepatitis C. Among this group 30 were treated with interferon-alpha (INF-alpha) during 6 months. Forty healthy children were included in the study as the control group. We examined subsets of blood lymphocytes T (CD3, CD4, CD8) and lymphocytes with expression of CD3/HLADR, CD3/CD25 antigens using monoclonal antibodies IMK plus and flow cytometry, at the beginning and after 6 months. After INF-alpha therapy a significant increase in subset of CD4 lymphocytes, significant decrease in CD8, increase in CD4/CD8 ratio and increase in lymphocytes with expression CD3/HLADR, CD3/CD25 receptors were observed. Differences in CD8 lymphocytes and CD4/CD8 ratio in the treated children were statistical higher in children with elimination HCV RNA. Applying INF-alpha in children with chronic hepatitis C stimulated immunological response by increasing subsets of CD4 lymphocytes, CD4/CD8 ratio and stimulated lymphocytes.

Antigens, CD↗
PubMed2001

Effect of IFN-alpha on total haemolytic activity of CH50 complement system and C3, C4 levels in children with chronic hepatitis C.

BACKGROUND: The pathogenesis of chronic hepatitis induced by virus C as well as the mechanisms responsible for the elimination of this infection are still not fully understood. The course of HCV infection depends on the extent to which both specific and non-specific response is induced. One of the treatment methods applied in HCV is the use of IFN-alpha which has both antiviral and immunomodulatory activity. The knowledge of IFN-alpha effect on non-specific humoral response may be helpful in the choice of optimal strategy for HCV prevention and treatment. The purpose of the study was the assessment of total haemolytic activity of CH50 complements as well as C3 and C4 levels in children with chronic hepatitis C treated with IFN-alpha. MATERIAL AND METHODS: Sixty children aged 3-15 years with chronic hepatitis C were included in the study. The diagnosis was based on biochemical examinations, the presence of HCV RNA and histological investigations of the liver. The study group was divided into two subgroups of 30 children each. One subgroup was treated with subcutaneous recombined IFN-alpha (Intron A Schering Plough) at the dose of 3 MU 3 times a week for 6 months, while the other subgroup was a control. All the children underwent the assessment of total haemolytic activity of CH50 complement according to modified Mayer's technique as well as the concentration of its components C3 and C4 with turbidimetric method with the use of Behring reagents and Turbi Time system. The assessments were performed at the beginning of the study and 6 months later. RESULTS: Total haemolytic activity of CH50 complement and C3, C4 levels after 6 months of IFN-alpha therapy were significantly higher in children on IFN-alpha when compared to control group. Among children treated with IFN-alpha, 9 managed to eliminate HCV genetic material from blood serum. Mean values of analysed parameters did not differ at both stages of the study in the IFN-alpha group between the children with and without HCV RNA elimination. CONCLUSIONS: Higher total haemolytic activity of CH50 complement and C3, C4 levels after 6 months of treatment with IFN-alpha administered to children with chronic hepatitis C suggest that IFN-alpha is capable of reducing complement activity.

Adolescent↗
PubMed2001

Autoimmune hepatitis in the course of chronic HAV--four year observation.

The paper presents a case of a 17-year-old girl with type 1 autoimmune hepatitis in whom biochemical, serological and histopathological markers of chronic hepatitis A were observed for the last 4 years. The diagnosis was based on elevated levels of gammaglobulins, IgG and the presence of autoantibodies (ANA, SMA). At first, the patient was treated with the pulses of corticosteroids and then, as no improvement was observed, continuous steroid therapy was involved. Relatively mild clinical course of the disease and good response to treatment with prednisone were recorded. Our observations suggest that HAV is capable of inducing autoimmune reactions and they indicate possible development of chronic hepatitis A.

Adolescent↗
PubMed2001

[Peripheral blood lymphocytes T gammadelta in children with acute acquired toxoplasmosis].

The aim of the study was to determine the percentage participation of lymphocytes CD3TCRgammadelta in peripheral blood of children with acute toxoplasmosis. The study compromised 30 children aged 2-14 years. Increase of the percentage of CD3TCR gammadelta cells was observed in the group of children with clinical symptoms of toxoplasmosis (mean value 5.7%) in relation to the comparative group (mean 4.4%), but in 12 children (40%) the mean value was 8.1%. Toxoplasma gondii infection produces a strong signal for activation of cells with gammadelta receptors. Examination of CD3TCRgammadelta cells may be helpful in the diagnosis of the acute phase of T. gondii infection.

Acute Disease↗
PubMed2001

[Congenital toxoplasmosis in own studies].

Clinical picture of congenital Toxoplasma gondii has been presented in 20 children. The infection was diagnosed in 18 children in the first and in 2 children in the sixth month of life. Clinical symptoms were observed in as many as 12 children. The most frequent symptoms were those concerning central nervous system, organ of vision in the form of hydrocephalus, spastic paresis, epilepsy, intracranial calcification, choroiditis, retinitis and vision impairment. The presented analysis indicates an urgent need of introduction of screening in pregnant women and infants and widespreading of prophylactic activity aiming at raising awareness of the existing threat of T. gondii infection.

Animals↗
PubMed2000

[Immunologic disorders in children with Lyme borreliosis].

In this study we present evaluation of some immune parameters in children with borreliosis. We examined subsets of blood lymphocyte (CD3, CD4, CD8, CD19, CD3 HLA DR) and NK cells using monoclonal antibodies and flow cytometry in 14 children age from 3 to 16 years. We observed an altered lymphocyte phenotypes and NK cells in infected children compare to healthy control. We conclude that the changes in host immune response may play an important role in the pathogenesis of borreliosis and their monitoring may be helpful for the prognosis the course of the disease.

Adolescent↗
PubMed2000

[Prognostic value of natural killer cells monitoring in the course of IFN-alpha therapy in children with chronic hepatitis C].

UNLABELLED: The aim of this study was evaluation of the role NK cells in antiviral response and the monitoring IFN-alpha therapy in children with chronic hepatitis C. The study included 60 children between 3 and 15 years of age with chronic hepatitis C--30 treated and 30 not treated IFN-alpha (Intron A, Schering Plough). Percentage of NK cells (CD3-CD16+CD56+) was determined in all children using monoclonal antibodies (Becton-Dickinson) and flow cytometry before and after therapy. RESULTS: After IFN-alpha therapy increase of the percentage NK cells, significantly higher in children with elimination HCV RNA and decrease NK cells in children no treated was observed. These observations underline significant part NK cells in antiviral response and suggest that IFN-alpha ability to increase this cells population is an important mechanism elimination of HCV infection. CONCLUSION: Increase NK cells in children with chronic hepatitis C IFN-alpha treated may be determinate effectiveness of IFN therapy.

Adolescent↗
PubMed2000

[Histological evaluation of activity and progression changes in the liver in HCV infected children].

In this study we present the analysis of pathological pictures of the liver in children with chronic HCV infection, among whom 60 children with HCV RNA and 22 children without HCV RNA were selected. For histological evaluation traditional classification and modified Histological Activity Index (HAI) was used. Only 2 children without viremia did not present changes in histopathological picture, in the remaining ones with and without viremia hepatitis chronic persistent prevailed. In both groups of children statistical differences in necroinflammatory activity and fibrosis were not observed. In histopathological picture correlation grading and staging was observed. The results of the studies show necessity for histological evaluation in anti-HCV carriers, because the pathology of the liver may occur independent of viremia.

Adolescent↗
PubMed1999

[Sources of infections and genotypes of hepatitis C virus in children].

The aim of the study was to analyse possible sources of HCV infection as well as to study their relation to specific HCV genotype. The study comprised 60 children with chronic hepatitis C age from 3 to 15 years. In 36.6% of patients, the probable risk factors were surgical interventions or injures; in 60% prior hospitalisation in conservative wards. Surgery was a risk factor in 59% of cases of infection with genotype 1a and in 41%--with genotype 1b. Hospitalisation conservative was the risk factor for the infection in 47% and 50% of cases, respectively.

Adolescent↗
PubMed1998

Expression of receptor Fc gamma RIII and phagocytic capacity of neutrophils in septic children.

Sepsis and its complications remain a frequent cause of death, but the pathogenesis of sepsis has not been thoroughly investigated. Ineffective elimination of the pathogen may be important in the development of a generalized inflammatory response. In this study, phagocytosis and the expression of Fc gamma RIII on neutrophils in peripheral blood from 25 children with sepsis and 25 healthy children were analyzed. The study was conducted in the initial phase of sepsis and then repeated 2-3 months after resolution. In the course of sepsis, a decrease in the percentage of neutrophils with Fc gamma RIII and the number of phagocytozing neutrophils compared with the control group were observed. Even after resolution, there was a decrease in the neutrophil phagocytic capacity. The persistent dysfunction of phagocytic cells in children after resolution of sepsis suggests the possibility of serious recurrent infections.

Bacteremia↗
PubMed1996

[Herpes simplex encephalitis in children].

Clinical course of Herpes Simplex Encephalitis were shown in this paper. 24 children in the age of 4 to 15 were treated at Polish Mother's Memorial Hospital in years 1992-1994. Value of EEG, CT scan, NMR were underlined in early diagnostic and casual treatment of diseases. All children from this analysis group survived, only 8 demonstrated late complications: epileptic attacks (3), spastic tetraparesis (1), encephalopathy and mental disturbances (1). Positive results of treatment were connected with early diagnosis and early antiviral treatment which is very important in decreasing death rate and preventing permanent injury.

Adolescent↗
PubMed1995

Eosinophilic gastroenteritis.

Eosinophilic gastroenteritis is a disease of rare incidence and unknown etiology. The descriptions of these rare cases refer to adults; there are few reports of children stricken with eosinophilic gastroenteritis. Our study presents a case of eosinophilic gastroenteritis in a 9-year-old girl suffering from recurrent abdominal pain, vomiting, diarrhoea, oedema of the lower extremities and ascites. The diagnosis was based on clinical features and confirmed by histopathologic examination of a biopsy specimen obtained from an upper gastroendoscopy. Food and parasitic allergens as aetiopathogenetic agents were taken into consideration.

Child↗