Biomedical subjects
Publications and source records attributed to T Y Basham.
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The T cell mitogens Pa-2, concanavalin A (con A) and its dimeric derivative succinyl-con A, were each cross-linked with the bifunctional reagent dimethyl suberimidate. Although the dose-response curves of these insoluble aggregated products were markedly changed from those of the soluble mitogens, each aggregate continued to stimulate DNA synthesis by murine thymus and T cells. Both aggregated Pa-2 and aggregated succinyl con A stimulated DNA synthesis by B cells from athymic (Nu/Nu) mice. Aggregated con A did not stimulate these cells and, like soluble con A, depressed the background incorporation of 3H-thymidine. Unlike soluble Pa-2, aggregated Pa-2 also greatly increased Ig production by both the B cell cultures and B + T cell cultures from normal (BALB/c) mice.
We have shown that of the five mitogens purified from the salt extracts of pokeweed, only Pa-1, the B cell mitogen, has the ability to stimulate immunoglobulin production in murine B cells in the absence of classical T cells. Furthermore, the T cell mitogens (Pa-2-Pa-5) not only are unable to stimulate DNA or immunoglobulin synthesis by B cells in the absence of T cells, but cannot stimulate immunoglobulin production by B cells even in the presence of T cells. This suggests that Pa-2-Pa-5 do not stimulate adequate helper function in T cells or that if such a helper function is stimulated, an additional stimulatory factor is required for immunoglobulin production, which is not provided for by Pa-2-Pa-5.
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