[Basic considerations and clinical aspects of pre- and postoperative radiation in lung cancer].
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Biomedical subjects
Publications and source records attributed to T YOSHIOKA.
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Experiments were carried out to determine the effect of storage at 37 degrees C on the allergenic potency for humans of dried BCG vaccine. From four lots of liquid vaccine, eight kinds of dried vaccine were prepared, four with 1% sodium glutamate and four with 1% sucrose as adjuvant. The eight vaccines were stored at 5 degrees C and at 37 degrees C for periods ranging from 1 month to 9 months, and were then used for the inoculation of primary-school children in Tokyo. These children were tuberculin-tested 1, 3 or 4 and, in a few cases, 12 months after vaccination, and the reactions were read approximately 48 hours after the tuberculin injection had been given.The tuberculin reactions induced by inoculation of the glutamate vaccines stored at 37 degrees C were, in all cases, appreciably stronger than those induced by the sucrose vaccines after storage at the same temperature for the same period. A slight decrease in the potency of the glutamate vaccines was observed after storage for 3 months at 37 degrees C, but storage for 1 month at this temperature did not appear to have any adverse effect on the potency.
Although many studies have been made on the lyophilization of BCG vaccine, few reports on its stability have appeared. The amount of living bacilli present in the vaccine and its allergenic potency can be considered to be an indication of the stability of the vaccine. The authors have already reported on the change in viability of vaccines preserved for 17 months; in this paper two experiments on lots of vaccines preserved for three years are discussed. The authors determined changes in viability after storage and examined tuberculin reactions one to eighteen months after vaccination. It was observed that the vaccines which had a relatively high potency at the time of lyophilization maintained their ability to induce allergy for at least two years.
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