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Biomedical subjects

T Yanai

Publications and source records attributed to T Yanai.

At least 73 records · Page 4Linked to original sources

Three-dimensional videography of swimming with panning periscopes.

The purpose of this paper is to describe a three-dimensional videography method with panning periscopes for reconstructing swimming techniques. Two panning periscope systems, developed in our laboratory, were used for the data collection. A control object (1 x 1.2 x 2)m3 with 72 markers was placed in seven consecutive locations along the (1.5 x 8.4 x 2) m3 calibration space. The position of the control object was recorded while panning the periscope systems, and for each of the seven locations the DLT camera parameters were determined for eight fields equally spaced through the panning motion. Each DLT camera parameter was then expressed as a function of the camera orientation by using a least-squares, second-order, polynomial regression equation, so that the DLT camera parameters for any camera orientation could be predicted (Yu et al., J. Biomechanics 26, 741-751, 1993). The three-dimensional coordinates of desired body landmarks at an instant were determined from the digitized two-dimensional coordinates for two cameras and the predicted DLT camera parameters at that instant. The accuracy of the method was evaluated by (a) the resultant errors in computing three-dimensional coordinates of precalibrated static points, and (b) the errors in computing the length of a scale rod pulled through the calibration space. The mean resultant errors ranged from 8.34 to 16.44 mm for the above- and from 9.93 to 16.22 mm for the below-water control volumes. The mean error in computing lengths on the scale rod ranged from 3.32 to 5.83 mm for three 0.5 m lengths, and 9.97 mm for a 1.5 m length. The method produced acceptable results in the reconstruction of three-dimensional motions recorded from a large space above and below the water surface.

Calibration↗

Immunohistochemical demonstration of S-phase cells by anti-bromodeoxyuridine monoclonal antibody in cattle tissues.

Bromodeoxyuridine (BrdU), a non-radioactive thymidine analogue, was administered to 15 cattle at a dosage of 1-10 mg/kg intravenously or intraperitoneally to demonstrate S-phase cells in the tissues. The organs and tissues were fixed in 10% neutral buffered formalin or in 70% ethanol, sectioned, denatured with hydrochloric acid, and treated with monoclonal antibody against BrdU. Immunohistochemical methods were used to "visualize" BrdU-labelled nuclei. BrdU-positive cells were satisfactorily demonstrated in both formalin- and ethanol-fixed tissues of animals given doses of 2 mg/kg or over, by either route of administration. Large numbers of BrdU-positive cells indicative of active cell production were found in the basal region of the stratified squamous epithelium, the neck between gastric pits and gastric glands in the abomasum, and the crypts of Lieberkühn of the small and large intestines. Moderate numbers of positive cells were observed amongst inflammatory cells in cases of nephritis and in granulation tissue. Numerous positive cells were detected in leukaemia cells. The study showed that BrdU can be used to measure proliferative S-phase cells in cattle, as in human beings, mice and rats.

Animals↗

Bilateral giant myelolipoma in the adrenal of a cotton-top tamarin (Saguinus oedipus).

A rare case of bilateral adrenal myelolipomas in a female cotton-top tamarin is reported. Large bilateral masses in the adrenal glands were composed of mature adipose cells containing varying amounts of hematopoietic cells of the myeloid, erythroid, and megakaryocytic series. The gross and histologic features of this case closely resemble human "giant" adrenal myelolipomas.

Adrenal Cortex↗

Effect of substrate on indirect immunofluorescence test for canine pemphigus foliaceus.

The effect of substrate on indirect immunofluorescence (IIF) tests for the detection of circulating autoantibodies was studied by examining sera from 14 canine pemphigus foliaceus patients, six sera with non-pemphigus dermatoses and ten normal dog sera against five different substrates from three species. These substrates included bovine esophagus, bovine nose, bovine tongue, monkey esophagus, and canine nose skin. Nine out of 14 (64.3%) sera from patients with canine pemphigus foliaceus showed intercellular space staining by indirect immunofluorescence using bovine esophagus as substrate. However, sera from nonpemphigus dermatoses and normal dog did not react with bovine esophagus. In other substrates, only bovine tongue showed 1/8 (12.5%) positive reaction at the intercellular space by sera from canine pemphigus foliaceus. Dog nose skin showed the intercellular space staining against ten of ten (100%) normal dog serum. Monkey esophagus showed the fluorescent deposit at the intercellular space in four of nine (44.4%) of pemphigus foliacues dog sera, however, four of ten (40%) of normal dog sera revealed nonspecificity intercellular staining. These results indicate that the sensitivity and the specifity of IIF test in canine pemphigus foliaceus depend on the substrate. The best substrate for detecting circulating autoantibody in canine pemphigus foliaceus patients among five different substrates was bovine esophagus because of its sensitivity and high specificity. The diagnosis of canine pemphigus foliaceus should be made on the basis of a combination of clinical signs, histopathology, direct immunofluorescence, and the detection of circulating autoantibody.

Animals↗

Ameloblastoma with prominent ossification in the mandible of a dog.

A rare case of ameloblastoma with prominent stromal ossification in an 8-year-old female dog was studied. A bony mass recurred rapidly in the right mandible at the first molar region. Histopathologic examination revealed the lesion to be an atypical variant of ameloblastoma. Epithelial cells showed marked cell atypia, and mitotic figures were rather common. The collagenous stroma was abundant, with prominent formation of bone trabecular rimmed by active osteoblasts. The tumor was highly proliferative and aggressive, and thought to be malignant in nature.

Ameloblastoma↗

Spontaneous vascular mineralization in the brain of horses.

Cerebral vascular mineralization was found in 12 (60%) of 20 3- to 10-year-old healthy horses collected at an abattoir. It was variable in degree and occurred mostly in the pallidal arteries showing two types of lesions; small globoid bodies along capillaries, and amorphous deposits in the wall of arterioles, small- or medium-sized arteries and veins. Both types were strongly positive for periodic acid-Schiff reaction, and weakly positive for von Kossa's and Berlin blue stains. Elemental analysis of the deposit revealed the presence of large amounts of aluminum, moderate amounts of phosphorus, zinc, calcium and iron, and a small amount of sodium.

Aging↗

Quantitative analysis of neuronal innervation in the aganglionic bowel of patients with Hirschsprung's disease.

Although there is marked proliferation of nerve fibers in the aganglionic bowel of patients with Hirschsprung's disease (HD), controversy exists as to whether these fibers terminate in the cells of the bowel wall. This study quantitates biochemically the synaptic vesicle proteins and neurofilaments in the aganglionic bowel of HD patients. The bowel specimens obtained from 12 patients with HD (mean age, 4.6 months) and nine age-matched controls were analyzed by Western blot analysis using monoclonal antibody 171B5 against the synaptic vesicle 38,000-d protein (SVP38), and the specific band obtained was quantitated by densitometry. Similarly, using monoclonal antibody NF70K for detecting the 70,000-d component of neurofilament (NF70), nerve fibers were quantitated. In the aganglionic HD bowel, the quantity of SVP38, representing a proportion of synapses, was significantly lower than that in the normoganglionic bowel (P < .001). The quantity of NF70, representing a proportion of nerve fibers, was significantly greater than that in the normoganglionic bowel (P < .05). These findings suggest that the nerve fibers in the aganglionic bowel of HD patients have much less developed connections with the cells of the bowel wall, indicating overall poor innervation, despite marked proliferation of the nerve fibers.

Blotting, Western↗

Eosinophilic neuronal inclusions in the thalamus of ageing B6C3F1 mice.

The age-related incidence of thalamic neuronal inclusions in the brains of aged B6C3F1 mice and their histopathological and ultrastructural features were studied. Round to oval or rod-shaped eosinophilic inclusions, which were frequently observed in the neurons of the thalamic area, were stained positively with phosphotungstic acid haematoxylin. The inclusions were detected first at 32 weeks of age and the incidence and severity were higher in older animals, all of the mice being affected after 58 weeks of age. Ultrastructurally, the inclusions appeared as sheaves of parallel osmiophilic filaments in the perikaryon of the neurons in the thalamus. Thus thalamic neuronal inclusions in mice seem to be age-related, but their significance remains unclear.

Aging↗

Subclinical renal oxalosis in wild-caught Japanese macaques (Macaca fuscata).

Various degrees of crystal deposition were found in the kidneys of 12 out of 59 Japanese macaques (Macaca fuscata) caught in the wild in Gifu, Japan. The needle- or rod-shaped crystals, which were radially arranged and occurred in the lumen and epithelium of the renal (mainly the proximal) tubules, were birefringent under polarized light. They stained with alizarin red S at a pH of 7.0 but not 4.2, and were identified as calcium oxalate. The morphological features of the renal lesions were similar to those previously reported in oxalate poisoning, and it was believed that the macaques ingested the oxalate in plants.

Animals↗

C1Q complexes with beta-2-microglobulin and amyloid P-component in sera of patients with dialysis-associated amyloidosis.

We studied complexes composed of C1q and beta 2-microglobulin (C1q.beta 2M) or serum amyloid P-component (C1q.SAP) in the sera of 38 patients receiving hemodialysis (HD) and 20 healthy control subjects using an enzyme-linked immunoassay. We also compared complex levels in HD patients with and without symptoms of HD-associated amyloidosis (HA). Serum C1q-beta 2M levels were significantly higher in HD patients without HA than in healthy control subjects and significantly higher in HD patients with HA than in HD patients without HA. Serum C1q.SAP levels were significantly lower in patients with HA than in those without HA. These results suggest that C1q.beta 2M may contribute to HA.

Amyloidosis↗

Basal cell tumor in a Japanese macaque (Macaca fuscata).

A rare case of basal cell tumor of the skin in a wild-caught female Japanese macaque (Macaca fuscata) was studied. Tumor growth, which was composed of cells resembling basal cells, was observed in the dermis and subcutis and showed a mixture of ribbon, solid, glandular, and cystic patterns. The tumor was separated from surrounding normal tissue by a compressed zone of fibrous connective tissue. The nuclei were round and hyperchromatic and possessed a single centrally located nucleolus. Mitotic figures were common. Immunohistochemically, a positive reaction for epithelial membrane antigen, keratin, and cytokeratin AE3 was evident. Electron microscopic examination revealed a small number of tonofilaments in the cytoplasm.

Animals↗

Separate control of the survival, the self-renewal and the differentiation of hemopoietic stem cells.

Hemopoietic stem cells adhere to hemopoietic supportive (MS-5) cells, but not to non-supportive (MS-K) cells. Although a soluble stem cell factor (SCF) was produced by both of these cell lines, little activity was detectable in the supernatant from the cultures of either of these cells, indicating that SCF might be compartmentalized within the extracellular matrix (ECM), and transferred directly to the stem cells via the ECM (44). To probe this possibility, we studied the transfer of SCF from the ECM and the subsequent support of the survival of the hemopoietic stem cells. A stem cell-enriched bone marrow cell fraction was overlaid on SCF-containing ECM. The stem cells survived and proliferated for some days without differentiation under these conditions, whereas stem cells overlaid on ECM without SCF died within a few days. Addition of interleukin-3 (IL-3) to the ECM that contains SCF, induced differentiation of the stem cells. Granulocyte-macrophage colony-stimulating factor (GM-CSF) induced further differentiation of the stem cells, which was accompanied by a decrease in the number of colony-forming unit in spleen (CFU-S). These observations verified the above hypothesis, and indicated that the survival, the self-renewal, and the differentiation of hemopoietic stem cells can be separately controlled at least in vitro.

Animals↗

A canine case of discoid lupus erythematosus with circulating autoantibody.

A nine-year-old Shetland sheepdog was diagnosed as discoid lupus erythematosus by clinical features, histopathologic findings, positive direct immunofluorescence, negative antinuclear antigen test and the absence of multisystemic diseases. The indirect immunofluorescence test of this patient dog with the salt split skin showed the deposition at the bottom of the cleft at basement membrane zone (BMZ). Western immunoblotting revealed the 120 kDa and the 85 kDa proteins targeted by the autoantibody. These proteins did not correspond with the known BMZ component.

Animals↗

Histopathologic features of naturally occurring hepatozoonosis in wild martens (Martes melampus) in Japan.

Nodular lesions containing Hepatozoon sp. schizonts or merozoite-gametocytes were found in the tissues of 67 (96%) of 70 wild-caught martens (Martes melampus) examined in Gifu, Japan, 1991 and 1992. The heart was the most commonly parasitized organ (96%), followed by the perirenal adipose tissue (36%); the diaphragm, mesentery, tongue, omentum and perisplenic adipose tissue generally had a prevalence of 10 to 15%. In the heart, two types of nodular lesions were differentiated based on developmental stages: nodules containing schizonts and nodules consisting of an accumulation of phagocytes containing merozoites or gamonts. Under electron microscopy, mature schizonts contained membrane-bound merozoites with a single nucleus and small scattered electron-dense cytoplasmic granules in the schizont nodules; the merozoites and gamonts were engulfed in a phagosome-like vacuole of phagocytes with the nucleus compressed to one side due to the parasite in the merozoite-gamont nodule.

Adipose Tissue↗

Polyglucosan bodies in the brain of a cow.

Polyglucosan bodies (PGBs) in the brain of a 12-year-old Holstein cow exhibiting no signs of neurological abnormality were examined by light and electron microscopy and immunohistochemistry. PGBs were disseminated throughout the brain, especially in the pallidum, thalamus and cerebellum. Cow PGBs were found in the neuronal perikaryon and in the neuropil. These were round, slightly to severely basophilic, and were strongly positive for the periodic acid-Schiff reaction. The cow PGBs were immunoreactive for monoclonal antibodies raised against human polyglucosan. Electron microscopic analysis revealed that they were composed of branching filaments, glycogen granules and electron-dense material. These findings indicate that cow PGBs closely resemble human or canine PGBs in Lafora's disease and PGBs found in aged animals that have been reported previously.

Animals↗