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Biomedical subjects

T Yasue

Publications and source records attributed to T Yasue.

At least 19 recordsLinked to original sources
PubMed1992

An immunoserological study of patients with vibration syndrome.

In a study undertaken to investigate the relationship between vibration syndrome and collagen disease, 90 Japanese patients with vibration syndrome (all men aged 41-86 years) were examined immunologic abnormalities, mainly by a set of immunoserology tests. Of these 90 patients, 25 had examinations at yearly intervals for 3 consecutive years, while the other 65 underwent examinations once. The results indicate that, of all the patients studied, 10 (11.1%) gave a positive RA test, 6 (6.7%) had leukopenia, 5 (5.6%) had hypergammaglobulinemia, and 15 (16.7%) had hypocomplementemia (CH50). Worthy of particular note are the 21 patients (23.3%) who were positive for nuclear-specific antibodies (1: greater than or equal to 40) (using Hep-2 cells as the nuclear substrate), with some of them suspected of having Sjögren's syndrome, progressive systemic sclerosis, or systemic lupus erythematosus. During a 3-year follow-up period, 10 (40%) of 25 patients exhibited rising titers of nuclear-specific antibodies with conversion to seropositivity for these antibodies. The facts that the positivity rate for nuclear-specific antibodies was significantly higher in these patients with vibration syndrome (23.3%) than in healthy adult men over 40 years of age (1.8%) (P less than 0.05) and that a progressive elevation of nuclear-specific antibody titer was noted in a high percentage of the patients were suggestive of some causal relationship between the appearance of nuclear-specific antibodies and the use of vibrating tools.

Adult↗
PubMed1992

Effectiveness of house dust-mite allergen avoidance through clean room therapy in patients with atopic dermatitis.

We have investigated the effectiveness of house dust-mite (HDM) allergen avoidance through clean room (CR) therapy in patients with atopic dermatitis (AD) having high HDM-specific IgE RAST levels. All patients (N = 30) demonstrated marked improvement in symptom, a long-term remission (mean, 8.4 months), and significant decreases in eosinophil count (p less than 0.01), basophil count (p less than 0.05), serum lactate dehydrogenase activity (p less than 0.01), and HDM-specific IgG levels (p less than 0.05). By contrast, the patients with AD (N = 11), who scored 0 on HDM allergen-specific IgE RAST score underwent "CR" therapy, and the patients with AD (N = 10) having high HDM-specific IgE RAST levels were hospitalized in a common sickroom (nonclean room). All the patients (N = 21) exhibited slight improvement in symptoms and a significant decrease in serum lactate dehydrogenase activity. The period of recurrence after the therapy was brief. The results of measurement of airborne house-dust particles indicated that our "CR" fell within class 10,000. Therefore, it is very likely that the mechanism at work in the CR therapeutic approach is its ability to eliminate fecal pellets of the HDM.

Air Conditioning↗
PubMed1992

In vitro and in vivo antibacterial activities of AM-1155, a new 6-fluoro-8-methoxy quinolone.

AM-1155 is a new quinolone with a wide spectrum of antibacterial activity against various bacteria including anaerobes and Mycoplasma pneumoniae. AM-1155 was 2- to 16-fold more active than ciprofloxacin and ofloxacin against Staphylococcus aureus including methicillin-resistant strains, Staphylococcus epidermidis, Streptococcus pneumoniae, and Enterococcus faecalis; its MICs for 90% of strains tested were 0.10 to 0.78 micrograms/ml. The activity of AM-1155 was comparable to that of ciprofloxacin against members of the family Enterobacteriaceae, Branhamella catarrhalis, Haemophilus influenzae, and Neisseria gonorrhoeae, but was fourfold less than that of ciprofloxacin against Pseudomonas aeruginosa. Against Xanthomonas maltophilia, Acinetobacter calcoaceticus, and Campylobacter jejuni, AM-1155 was two- to fourfold more active than ciprofloxacin. At a concentration of 1.56 micrograms/ml, AM-1155 inhibited 90% of Bacteroides fragilis strains tested; its activity was 8- to 10-fold higher than those of ofloxacin and ciprofloxacin. Development of resistance to AM-1155 in S. aureus and S. epidermidis occurred at a lower frequency than did that to ciprofloxacin after eight transfers in the presence of drug. In the oral treatment of mouse systemic infections, AM-1155 was four- to eightfold more effective than ciprofloxacin against gram-positive cocci and was as active as ciprofloxacin against gram-negative rods. The efficacy of an oral or a subcutaneous dose of AM-1155 was two- to fivefold greater than that of ofloxacin. Against experimental pneumonia with Klebsiella pneumoniae and P. aeruginosa, AM-1155 was two- to fourfold more active than ciprofloxacin and ofloxacin. AM-1155 also had good efficacy against mouse ascending urinary tract infections with Escherichia coli and P. aeruginosa. These results suggest that AM-1155 may be a potent antibacterial agent applicable to various infections.

Animals↗
PubMed1991

Functional analysis of HLA-DR-expressing keratinocytes from tuberculin reactive skin.

We examined the functional roles of HLA-DR+ keratinocytes which were induced in vivo from tuberculin reactive skin. At 4 days after intradermal PPD injection, about 80% of keratinocytes obtained from the tuberculin reactive area expressed DR antigens. In 14 of 18 individuals examined, PPD-pulsed DR+ keratinocyte fraction induced autologous T-cell proliferation. The proliferative response was PPD-dependent, antigen specific; it depended upon DR expression by the keratinocyte fraction, because it did not occur in the presence of the PPD-nonpulsed DR+ keratinocyte fraction and was completely blocked by mouse monoclonal antibody to HLA-DR. However, the antigen-presenting capacity of the DR+ keratinocyte fraction appeared to be less than that of monocytes or the DR+ epidermal cell fraction. The DR+ keratinocyte fraction was also able to stimulate allogeneic T-cell DNA synthesis, but the DR- keratinocyte fraction could not. The possible influences of contaminant Langerhans cells and mononuclear cells in the DR+ keratinocyte fraction were considered to be unlikely. These results suggest that DR+ keratinocytes, which are induced in vivo, can both present the antigen to autologous T cells and stimulate allogeneic T cells.

Cell Division↗
PubMed1990

Cytotoxic effect of 1-methyl-4-phenylpyridinium ion on human melanoma cell lines, HMV-II and SK-MEL-44, is dependent on the melanin contents and caused by inhibition of mitochondrial electron transport.

MPP+, an oxidative metabolite of a neurotoxin, MPTP, was found to be cytotoxic to human melanoma cell lines, HMV-II and SK-MEL-44. After 3 days of culture in the presence of MPP+, a larger amount of MPP+ was accumulated in HMV-II cells than in SK-MEL-44 cells, which correlated well with the melanin contents; HMV-II cells contain larger amounts of melanin than SK-MEL-44 cells. After 6 days of culture in the presence of MPP+, the cytotoxicity of MPP+ on these cell types was evaluated by counting cell numbers with the dye exclusion test and double-layer soft agar clonogenic assay. It was found that exposure to MPP+ reduced the survival of HMV-II cells more significantly than that of SK-MEL-44 cells. In HMV-II cells, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was used to elucidate the mechanism of MPP+ lethality. The formazan formation was reduced markedly by the presence of MPP+ at concentrations much lower than those required for cell death. These results suggest that cytotoxicity of MPP+ may be ascribed to its accumulation due to high affinity for melanin, and to inhibition of the enzymes utilizing ubiquinone in the mitochondrial respiratory chain.

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine↗
PubMed1990

[Porphyria cutanea tarda in a chronic hemodialysis patient].

After 6 months on hemodialysis, a 58-year-old male patient with diabetes developed photosensitive bullous dermatosis on his hands and face. There was no evidence of liver diseases although the patient had a history of excessive alcohol consumption. He was suspected to have iron overload in the liver. Analysis of porphyrins in plasma, hemofiltrate, urine and feces by high performance liquid chromatography revealed significantly high levels in these samples with a porphyrin profiles which is consistent with porphyria cutanea tarda. The fluorometric assays of plasma also disclosed an elevated plasma porphyrin level. And it seemed that there were correlation between the fluctuation of vesicle formation, serum ferritin level and plasma porphyrin level. Small volume plasma exchange temporarily reduced the plasma porphyrin level and prevented vesicle formation.

Chromatography, High Pressure Liquid↗
PubMed1989

[Two cases of scleroderma associated with vibration syndrome].

Case 1, a 49-year-old male who had been engaged in repair and reclamation of automobile tires, developed symptoms of vibration syndrome (Raynaud's phenomenon, numbness of both hands, tinnitus and impaired hearing) after some 30 years' use of a grinder and impact wrench. Two years thereafter, multiple sclerodermic lesions appeared over the trunk, upper extremities, and thighs; these disappeared about 2 years later. Histologically, hyperplasia and nodular swelling of collagen bundles were present in the dermis. An immunological study showed the serum to be positive for anti-centromere antibody, but no visceral lesions were demonstrable. This case corresponded to generalized morphea. Case 2, a 53-year-old male, developed symptoms of vibration syndrome (Raynaud's phenomenon, numbness of both hands, impaired hearing and arthralgia) after 25 years' use of a jack hammer in a quarry. Thereafter, sclerodermic changes of the forearms, lower legs, face and abdomen occurred with an associated sclerodactyly. Histological examination of involved skin revealed diffuse hyperplasia and homogenization of collagen bundles throughout the entire thickness of the dermis. These findings, together with serum positivity for anti-RNP antibody and dilation of the lower portion of the esophagus, led us to a diagnosis of progressive systemic sclerosis. We inferred that the vibration syndrome in the present cases might be related etiologically to these forms of scleroderma.

Autoantibodies↗
PubMed1989

[Neoplastic angioendotheliosis: a case of T cell lymphoma positive for S-100 protein].

A 63-year-old woman presented with painless, firm, subcutaneous nodules on her legs and trunk. The lesions were annular or serpiginous and their surface was livid-red to pale-red. Superficial lymph nodes were not palpable. Upper gastrointestinal series, barium enema, Ga scintigraphy, and CT findings were negative. Histological examination revealed deep dermal vessels occupied by neoplastic cells and fibrin thrombi. We diagnosed her as having neoplastic angioendotheliosis. Electron microscopically, tumor cells lacked Weibel-Palade bodies. Immunohistochemically, the tumor cells were positive for leukocyte common antigen, T11, MT-1, HLA-DR and S-100 protein. They were negative for Factor VIII-related antigen, epithelial membrane antigen, carcinoembryonic antigen, B-1, and B-2. Immunoelectron microscopic study showed that their nuclei, cytoplasma, and cell membranes were positive for S-100 protein. Based on these findings, we diagnosed her as having T cell lymphoma. The patient rejected chemotherapy and was discharged. Three months after discharge, right hemiplegia developed. Brain CT scan revealed enhanced masses in the left frontal, temporal, and occipital lobes. Craniotomy showed only gliosis and hemorrhage. She died of cerebral bleeding three months after craniotomy.

Biomarkers, Tumor↗
PubMed1989

[The roles of proteinase production and germ tube formation in the invasion of Candida albicans into newborn mouse skin].

The pathogenic factors of Candida albicans involved in invasion of the skin were studied. Strains of C. albicans differing in their abilities to secrete proteinase in vitro and to produce germ tube were inoculated onto the skin surface of newborn mice, and the invasion of the yeast cells into the horny layer was examined by histological techniques. The strains capable of producing germ tubes could penetrate into the skin, but those incapable of germ tube formation could not. The penetration of the yeast cells into the skin was independent of the ability to secrete proteinase, but, once penetrated, Candida cells from the proteinase-producing strains proliferated more profusely than those of proteinase-deficient ones. The results suggested that the invasion of C. albicans into the skin consisted of two processes; penetration and proliferation of the yeast cells in the tissue. The former might depend on the ability of germ tube formation and the latter on the secretion of proteinase.

Animals↗
PubMed1988

Effects of fleroxacin on HeLa cell functions and topoisomerase II.

The effects of fleroxacin and other new 4-quinolones on cellular functions and topoisomerase II of HeLa cells were investigated. The potency of cytotoxicity increased in the order fleroxacin less than nalidixic acid less than ofloxacin less than norfloxacin less than ciprofloxacin. The cytotoxicity was well correlated with inhibitory effects of 4-quinolones on topoisomerase II. However, the cytotoxicity did not correlate with antibacterial activity, and cytotoxicity was observed only at greater than 100-fold higher concentrations than the minimum inhibitory concentrations of new 4-quinolones for Enterobacteriaceae. Judging from the serum levels attainable after oral administration, new 4-quinolones, especially fleroxacin, will have little effect on topoisomerase in eukaryotic cells.

Anti-Infective Agents↗
PubMed1987

Angiographic difference in coronary artery of man, dog, pig, and monkey.

Comparative morphological studies on the coronary arteries of the left ventricular free wall were carried out on human, dog, pig, and monkey hearts by using postmortem coronary arteriography, soft X-ray photograms, and the clearing method. The results showed that the types of coronary arteries (types I, II, and III) and connecting portion of anastomotic vessels in the pig and monkey hearts closely resembled those in man. Whereas the dog hearts showed the following characteristics: numerous Type III vessels and anastomoses in the epicardial layer, the existence of only the left predominant type of coronary artery, and the supply of blood to the papillary muscles of the left ventricle mostly through a single branch of the coronary artery. Therefore, it is necessary to take into consideration the basic difference in the structure of the coronary arteries of human and dog hearts, when dogs are used experimentally for research of human ischemic heart disease. The fact that only the papillary muscles of the human heart-compared to animal hearts-are supplied blood from two sources may be advantageous to rescue the papillary muscles from ischemic necrosis.

Adolescent↗