[A case of pancreatic ascite with preoperative diagnosis of leaking pancreatic cyst].
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Ascites↗
Biomedical subjects
T Yoneyama
Publications and source records attributed to T Yoneyama.
Intraglomerular fibronectin in rat experimental glomerulonephritis.
To clarify the mechanisms of glomerular pericapillary fibronectin deposition in human membranous nephropathy and mesangial proliferative glomerulonephritis, intraglomerular fibronectin distribution was examined by light and electron microscopy using the experimental rat models of Heymann and nephrotoxic serum nephritis. As previously demonstrated by immunofluorescence microscopy (Pettersson and Colvin 1978; Ikeya et al. 1985, 1986), fibronectin was distributed in the mesangial areas and occasionally on percicapillary walls of normal glomeruli, while in nephrotoxic serum nephritis and Heymann nephritis, fibronectin was diffusely located along glomerular capillary walls as well as in the mesangium. By immunoelectron microscopy using the immunogold technique, fibronectin was also noted in the mesangial areas and the lamina densa of the glomerular basement membrane (GBM) in normal glomeruli. In nephrotoxic serum nephritis, fibronectin was seen around mesangial cells situated between endothelial cells and the GBM, suggesting that pericapillary fibronectin in nephrotoxic serum nephritis reflects mesangial extension. However, in Heymann nephritis, it was found uniformly in the lamina rara interna, lamina densa and lamina rara externa of the GBM, indicating no specific relation to glomerular cells. When sections of normal and both experimental nephritis kidneys were incubated with fluorescein isothiocyanate conjugated with rat plasma fibronectin, a linear pattern of fluorescein staining along the glomerular capillary walls was observed in Heymann nephritis but not in normal or nephrotoxic serum nephritic rats. The GBM in Heymann nephritis would thus appear to have an affinity for plasma fibronectin. Based on the above findings, fibronectin in the GBM of rats with Heymann nephritis may reasonably be concluded to originate from the plasma.
Animals↗
Elevated serum levels of creatine kinase BB in autosomal dominant osteopetrosis type II--a family study.
A markedly elevated BB isoenzyme fraction of serum creatine kinase was noted in four male siblings and correlated with typical radiographic findings of autosomal dominant osteopetrosis Type II (ADO Type II). Patients with other sclerosing bone diseases had no elevation of CK-BB. The precision of the electrophoretic mobility patterns and correlation by I-125 tagged radioimmunoassay method confirms that this is CK-BB. We postulate that the dysfunctional and/or immature osteoclasts in ADO are more dependent on CK-BB than on the usual tricarboxylic acid cycle for the production of energy. The correlation of marked elevation of serum CK-BB with radiographic evidence of ADO Type II may prove to be of value as a biologic marker in the early diagnosis of the illness and lead to better understanding of the metabolism of bone.
Aged↗
Super-elasticity and thermal behavior of Ni-Ti alloy orthodontic arch wires.
Bending properties and thermal behavior of twenty commercial Ni-Ti alloy orthodontic arch wires were investigated quantitatively to characterize their suitability for clinical use. There was substantial difference among the load-deflection curves obtained by a three-point bending test. Some wires exhibited super-elasticity; load decreased little with decreasing deflection. Others showed good spring-back properties only; load was nearly proportional to deflection. Thermal behavior due to phase transformation of the alloy was examined by differential scanning calorimetry (DSC). Some of the wires did not have the correct transformation temperatures to exhibit super-elasticity at body temperature. Moreover, thermal behavior was closely related to super-elasticity. There were clear thermal peaks in the DSC curves of the super-elastic wires. However, wires without super-elasticity had no peak in the DSC curves.
Dental Alloys↗
Influence of composition and purity on tensile properties of Ni-Ti alloy castings.
The influence of composition and purity of titanium on the mechanical properties and the transformation temperatures of Ni-Ti alloy dental castings was investigated by tensile testing and differential scanning calorimetry (DSC). The compositions of the ingots were 49.0-49.2 at% of titanium content. Three grades of titanium of relatively high purity were used as starting materials. The result showed Ni-49.0Ti to be a somewhat brittle property, and Ni-49.2Ti to have low apparent proof strength and large elongation. Residual strain increased with increasing titanium content. Even small reductions of titanium purity influenced the tensile properties and the transformation temperatures, causing high apparent proof strength, low residual strain and low elongation because of the reduction in transformation temperatures.
Dental Alloys↗
[A case of metastatic urinary bladder tumor from gastric carcinoma].
We report a case of metastatic bladder tumor from gastric carcinoma. A 55-year old male patient was referred to our urological clinic with a complaint of frequent urination and voiding pain. He had undergone total gastrectomy for poorly differentiated adenocarcinoma, signet-ring cell type, 9 months earlier. Computed tomographic scan revealed a thick bladder and rectum wall all around. Punch biopsies from vesical and rectal wall revealed metastatic adenocarcinoma, signet-ring cell type. There were no other metastatic sites. Systemic chemotherapy was done with a combination of mitomycin-C, 5-fluorouracil and cytosine arabinoside. This chemotherapy was effective and complete remission was obtained at bladder and rectum. Six months after chemotherapy, peritoneal recurrence developed and he died 9 months after chemotherapy. However no recurrence of bladder tumor was detected. This was a quite rare case of metastatic bladder tumor characterized by good response to systemic chemotherapy.
Adenocarcinoma, Mucinous↗
Clinical risk indicators for periodontal attachment loss.
The purpose of the present investigation was to evaluate the association of baseline clinical parameters of periodontal disease with disease progression in the following year. 271 randomly selected subjects from Ushiku Japan were monitored for overt gingivitis and plaque accumulation at 4 sites per tooth and probing pocket depth, probing attachment level and bleeding on probing at 6 sites per tooth for all teeth at baseline and 1 year. A subject was considered to exhibit additional attachment loss if one or more sites increased 3 mm or more in a probing attachment level measurement in one year. The clinical variables included age, sex, number of missing teeth, mean pocket depth and attachment level. In addition, the % of sites which exhibited overt gingivitis, visible plaque, pocket depths, attachment levels or gingival recession over certain mm thresholds or bled on probing were determined. Chi 2 analysis was used to seek significant associations between the baseline clinical variables and subsequent attachment loss in a subject. Only 74 of the 271 subjects (27.3%) exhibited additional attachment loss of 3 mm or more at 1 or more sites after 1 year. Older subjects had a greater risk of disease progression than younger subjects. There were no significant differences in % of individuals exhibiting additional attachment loss when divided on the basis of sex, number of missing teeth or % of sites with overt gingivitis. However, the greater the % of sites with visible plaque or which bled on probing, the greater was the likelihood of subsequent attachment loss. Increasing mean levels of pocket depth or attachment level or increasing %s of sites exhibiting prior attachment loss were strongly related to the proportion of individuals with subsequent attachment loss. Gingival recession exhibited similar but weaker relationships. Log-linear analysis suggested that the association between bleeding on probing, age, or plaque levels with additional attachment loss may be explained by the association of these variables with baseline attachment loss. The analyses were repeated with a positive subject defined as having only 1 active site or 2 or more active sites. 37 subjects fit the 1st criterion and the remaining 37 the 2nd criterion. The associations observed were almost identical to those found when subjects were considered positive on the basis of 1 or more changing sites. Discriminant analysis was used to classify subjects as active or inactive using up to 11 predictor variables.(ABSTRACT TRUNCATED AT 400 WORDS)
Adult↗
Discrimination of hepatitis C virus in liver tissues from different patients with hepatocellular carcinomas by direct nucleotide sequencing of amplified cDNA of the viral genome.
We investigated the presence of the hepatitis C virus (HCV) genome in liver tissues of eight different patients with hepatocellular carcinoma by using the reverse transcriptase polymerase chain reaction (PCR) method. RNA was extracted separately from cancerous and peripheral noncancerous portions of the liver tissues of each patient. For reverse transcriptase PCR, we used sets of primers derived either from nonstructural region 3 (the NS3 region) or from the nucleocapsid-envelope (C/E) region of the HCV genome. The nucleotide sequences of the amplimers were directly determined without subcloning. Of 16 samples tested, cDNA of the HCV genome was detected in 2 cancerous tissues and in 4 noncancerous tissues by either pair of primers. Nucleotide sequences of HCV cDNA fragments amplified from cancerous and peripheral noncancerous tissues from the same patients were identical. However, 4.4 to 6.3% and 7.5 to 11.3% sequence variation was observed in NS3 and C/E regions, respectively, among cDNA fragments from different patients. The result indicated that the HCV genome detected in a given patient is distinguishable from that in others by a simple direct nucleotide sequencing of the reverse transcriptase PCR products.
Base Sequence↗
Therapeutic efficacy of liposome-encapsulated kanamycin against Mycobacterium intracellulare infection induced in mice.
Liposome-encapsulated kanamycin (KM) was examined for therapeutic efficacy against experimental infection induced with Mycobacterium intracellulare in mice. Liposomal KM injections (once weekly for up to 8 wk) led to a greater reduction in the degree of gross pulmonary lesions and in the growth of the organisms in the visceral organs (lungs, liver, spleen, and kidneys) of infected mice than did either free KM alone or free KM mixed with empty liposomal vesicles. The therapeutic effect of the liposome-encapsulated KM was dose dependent for doses from 50 to 200 micrograms KM/mouse/injection. Liposome encapsulation markedly changed the tissue distribution of KM, in particular in the reticuloendothelial organs, such as liver and spleen. In these organs, accumulation and retention of liposome-entrapped KM was remarkably higher than that of free KM. Moreover, considerably prolonged retention was seen for liposome-encapsulated KM in serum and lungs. Encapsulation of KM into liposomal vesicles increased incorporation of the drug into the host peritoneal macrophages and enhanced the antimicrobial activity of the agent against M. intracellulare phagocytosed into macrophages.
Animals↗
Diabetic environment and genetic predisposition as causes of congenital malformations in NOD mouse embryos.
Congenital malformations such as neural tube defects and a kinky or waved vertebral column were observed at higher incidence in embryos from nonobese diabetic (NOD) female mice with overt diabetes (NOD-D; 40.3%, P less than 0.005) or without overt diabetes (NOD-N; 8.4%, P less than 0.05) than in control Institute of Cancer Research (ICR) mouse embryos (1%) at day 13 of gestation. In vivo and in vitro preimplantation development of NOD-N, NOD-D, and ICR embryos did not differ in rate of development, size, or morphology. Embryos cultured from one-cell to early blastocyst stage were mutually transferred to uterine horns of pseudopregnant females between NOD-D and ICR mice and examined at day 13 of gestation. There were significant decreases in ratios of implantation and of viable embryos in ICR embryos transferred to NOD-D recipients (52%, P less than 0.001 and 14%, P less than 0.001, respectively) compared with those ratios in ICR embryos transferred to ICR uteri (79.2 and 56.2%) or those in NOD-D embryos transferred to ICR uteri (70.3 and 33.1%). Furthermore, 18 of 45 viable ICR embryos transferred to NOD-D dams had malformations, whereas there were no malformations in 73 viable ICR embryos transferred to ICR recipients, suggesting deleterious effects of maternal diabetic environment to embryos. On the other hand, 8 of 58 viable NOD-D embryos that were cultured in vitro and transferred to ICR uteri had malformations such as neural tube defects.(ABSTRACT TRUNCATED AT 250 WORDS)
Animals↗
Simple method for preparing a concentration gradient of serum components by freezing and thawing.
We created a simple method for obtaining a series of successively more-concentrated samples from a serum without changing the ratio of its components. We froze a pooled serum and then allowed it to thaw undisturbed. The serum components formed a gradient of increasing concentration from the top of the sample to the bottom. We found that (a) in test results, each fraction of serum in the gradient showed almost the same relative concentrations of components (i.e., inorganic and organic compounds, proteins, metals, and hormones), irrespective of atomic or molecular mass; (b) the concentration gradient depended on the thawing temperature but not on the freezing temperature; (c) when we thawed the frozen sample with centrifugation, the slope of the concentration gradient increased with increasing centrifugal force; (d) when the thawed sample was fractionated into 10 fractions from the top to the bottom, the original serum concentration was always maintained between the sixth and seventh fractions from the top; and (e) the concentration gradient became steeper with repeated freezing and thawing. By using this method, one can easily prepare serum samples at gradients of concentration useful in the clinical laboratory, although the mechanism of gradient formation is still unclear.
Blood↗
Detection of hepatitis C virus cDNA sequence by the polymerase chain reaction in hepatocellular carcinoma tissues.
We found the presence of hepatitis C virus (HCV) infection in liver tissues of hepatocellular carcinoma (HCC) patients who had antibodies to HCV but no serological markers for hepatitis B virus infection by the sensitive reverse transcription/polymerase chain reaction (R/PCR) method. The primers used were derived from the non-structural (NS) 3 and/or the structural (C/E) region. Amplified cDNA sequences of HCV were detected in either cancerous or non-cancerous portion of liver tissues from four out of eight HCC patients with primers of NS3 region. Similar but less efficient results were obtained with primers of C/E region. These results indicate that HCV persists in the liver tissue of HCC. A possible role of persistent infection of HCV for the development of HCC is discussed.
Base Sequence↗
Molecular mechanism of mechanical stress in bone and periodontium.
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Bone Remodeling↗
Hepatitis B surface antigen expressed in murine cells.
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Animals↗
Elevated levels of creatine kinase BB isoenzyme in three patients with adult osteopetrosis.
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Adult↗
Single vaginal ectopic ureter via Gartner's duct cyst spontaneously perforating into the bladder.
We report on a girl with a rare variant of single vaginal ectopic ureter via Gartner's duct cyst, which ruptured spontaneously into the bladder leading to aggravation of urinary incontinence. The patient also had a megalovagina, and a fistula between the distal urethra and vagina. She became continent after removal of a dysplastic kidney, unroofing of the cyst and closure of the fistulas.
Child↗
A sensitive method for the detection of residual cell DNA in a recombinant hepatitis B vaccine prepared from Chinese hamster ovary cells.
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Animals↗
Longitudinal changes in periodontal disease in untreated subjects.
About 300 subjects, 20-79 years of age were recruited for a longitudinal study on the effect of periodontal therapy. The result of a baseline examination have been reported earlier. Following this baseline examination, the subjects were monitored for 24 months without therapy. Re-examinations were performed after 12 and 24 months. All teeth including the 3rd molars were included in the examinations. Presence of plaque was assessed at 4 surfaces per tooth and gingivitis, probing pocket depth, probing attachment levels were assessed at 6 locations per tooth. Out of the subject sample examined at baseline, 57 individuals failed to return for either the 1st, the 2nd or both re-examinations. An analysis was performed regarding the periodontal status at baseline, of the respondents and non-respondents. The results from the follow-up examinations of the participating 20 to 79 year-old subjects revealed that the sample underwent, during a 2-year period, only minor changes with respect to a series of different parameters characteristic of periodontal disease. Thus, the mean values of probing pocket depth and probing attachment level failed to change between baseline and the re-examinations after 1 and 2 years. Even if the mean values underwent only minor changes, however, certain subjects within each age category improved their periodontal conditions, whereas other subjects worsened. Furthermore, the findings of the re-examinations revealed that there was a strong correlation between improving plaque levels and gingivitis. The relationship between supragingival plaque levels and changes with respect to probing depth and attachment levels were weak.
Adult↗