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Biomedical subjects

Takao Akatsuka

Publications and source records attributed to Takao Akatsuka.

7 recordsLinked to original sources

Hard-x-ray region tomographic reconstruction of the refractive-index gradient vector field: imaging principles and comparisons with diffraction-enhanced-imaging-based computed tomography.

The unique tomographic imaging method based on refractive effects that was recently developed by Maksimenko et al. [Appl. Phys. Lett. 86, 124105 (2005)] exhibits an excellent imaging property in the hard-x-ray region for phase objects such as soft materials and biological samples. However, there seems to have been little consideration of the physical aspects of the underlying imaging principles. Also, as the method is similar to diffraction-enhanced-imaging (DEI)-based computed tomography (CT), the difference between these two methodologies has not been made clear. We theoretically consider the imaging principles starting from the measurement process to the reconstruction procedures from the viewpoint of geometrical optics and then clarify their difference in relationship to the physical quantities to be depicted. The major feature of this novel method is the in-plane two-dimensional vector-field reconstruction of the refractive-index gradient in an object, while DEI CT obtains the out-of-plane scalar-field gradient component. In other words, the novel method and DEI CT present the transverse and the longitudinal components, respectively, of the three-dimensional vector fields of the gradient refractive index. Therefore they can be considered complementary to each other.

Algorithms↗

Normalizing renal reducing ability prevents adriamycin-induced proteinuria.

Reactive oxygen species play an important role in adriamycin (ADR) nephropathy. We showed by in vivo electron paramagnetic resonance (EPR) that renal reducing ability (RRA) declined on the 7th day after ADR administration. Proteinuria appeared after the decline in RRA. The aim of this study was to prove by in vivo EPR whether the decline in RRA is altered by scavengers such as dimethyl sulfoxide (DMSO) and dimethylthiourea (DMTU) and that it is this change which is responsible for the proteinuria in ADR nephropathy. By showing that DMSO and DMTU ameliorate the RRA, we demonstrate that the decline in RRA is related to ADR-induced proteinuria.

Animals↗

Evaluation of adriamycin nephropathy by an in vivo electron paramagnetic resonance.

A rat model for human minimal change nephropathy was obtained by the intravenous injection of adriamycin (ADR) at 5 mg/kg. By using an in vivo electron paramagnetic resonance (EPR) spectrometer operating at 700 MHz, the temporal changes in signal intensities of a nitroxide radical, 4-hydroxyl-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPOL), in the kidneys of rats with ADR nephropathy were investigated. The decay rate of the EPR signal intensity obtained in the kidney is indicative of the renal reducing ability. It was found that the reducing ability in the kidney declined on the 7th day after ADR administration and recovered after the 14th day. Impairment of the reducing ability occurred before the appearance of continuous urinary protein. The in vitro EPR study showed that this impairment of in vivo renal reducing ability is related to impairment of the reducing ability in the mitochondria.

Animals↗

High-pass-filtered diffraction microtomography by coherent hard x rays for cell imaging: theoretical and numerical studies of the imaging and reconstruction principles.

This paper presents theoretical and numerical studies of diffraction tomography using hard x rays, from the viewpoint of imaging and reconstruction methods for cell imaging. The proposed system employs a single-perfect-crystal analyzer in symmetric Laue-case transmission geometry to efficiently detect the higher spatial frequency components of an object's refractive-index distribution, and to effectively suppress interference between the unperturbated wave field and the wave field diffracted by the object. This system features acquisition of a single projection by a single exposure using a simple geometry and aggressive use of diffracted x rays. We present the physical description of the imaging method using the Fourier diffraction theorem derived from the Born approximation. First, we demonstrate that the reconstruction leads to the phase-retrieval problem. We then describe a reconstruction algorithm based on the classical Gerchberg-Saxton-Fienup algorithm. Finally, we show the efficacy of this system by computer simulation. Our simulation demonstrates that the imaging system delineates microstructure 3.5 microm in diameter in a phase object 400 microm in diameter.

Algorithms↗

Estimation of temporary change of activation areas by moving an analysis time window in fMRI measurement.

In this paper, we propose a method to acquire temporal changes of activations by moving an analysis time window. An advantage of this method is that it can acquire rough changes of activated areas even with the data having low time resolution. We ascertained that activations from our method do not contradict previous reports on the oddball paradigm, thus showing its effectiveness. Eight normal subjects participated in the study, which consisted of a random series of 30 target and 70 nontarget stimuli. We investigated the activated area in three kinds of analysis time sections, from stimulus onset to 5 s after the stimulus (time section A), from 2 to 7 s after (B) and from 4 to 9 s after (C). In time section A, representative activated areas were regions including the left and supplementary motor areas (SMA), and cerebellum. In B, regions including the left motor area and SMA, right parahippocampal gyrus (Broadmann Area (BA) 30), right limbic lobe and cerebellum were activated. In C, bilaterally postcentral gyrus (BA 3,40), right anterior cingulate (ACC, BA 32), left middle frontal gyrus (BA 9) and right parahippocampal gyrus were activated. Most activations were consistent with previous studies.

Adult↗

Noninvasive in vivo determination of intracerebral oxygen concentration in rats by a longitudinally detected ESR (LODESR) technique.

We have developed a noninvasive method to determine oxygen concentration in the brain tissue of rats in vivo. The method is based upon measuring the fundamental harmonic-to-secondary harmonic ratio (FSR) of longitudinal magnetization changes of a blood-brain barrier (BBB)-permeable nitroxide radical, 3-hydroxymethyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (hydroxymethyl-PROXYL), by employing a longitudinally detected ESR (LODESR) spectrometer operating at an ESR frequency of 280 MHz. FSRs of phantoms, including a hydroxymethyl-PROXYL solution and various concentrations of oxygen, were measured. We found that the FSRs of the phantom increased as the oxygen concentration increased. In vivo FSRs in the brains of rats that had received a hydroxymethyl-PROXYL injection were measured without the use of any surgical procedures. It was found that when the rats breathed 100% oxygen, rather than normal air, the FSR was significantly greater.

Animals↗

Fundamental imaging properties of transillumination laser computed tomography based on coherent detection imaging method.

The coherent detection imaging (CDI) method uses the optical heterodyne detection technique. CW and single frequency lasers having long coherence lengths are used to exploit the maximum advantages of heterodyne detection, such as high directionality, selectivity and sensitivity. The CDI method based on optical heterodyne detection enables selective filtering of the directional coherence-retaining emergent photons, which leads to image reconstruction from projections, similar to X-ray computed tomography (CT). So far we have demonstrated the advantages and capabilities of the measurement technique for transillumination optical computed tomography in biomedicine. Here, we investigate the fundamental imaging properties of CDI method, such as its high directionality and quantitativeness, with preliminary physical phantom experiments. The results show that the CDI method satisfies the requirements for CT reconstruction under the first order approximation, and enables quantitative measurements in the sense that the relationship between estimated and actual concentration retains a satisfactory linearity.

Equipment Design↗