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Tang-Ni Qin

Publications and source records attributed to Tang-Ni Qin.

2 recordsLinked to original sources

Comparing the base usage frequency between bacteria DNA double strand.

During the Evolution, effected by select pressure or nature mutation, the compositions of bacteria genomes is various. And many experiences prove that the genes between leading strand and lagging strand is distinctly in copy, transcription and repair. Some scholars presume that the bases distribution is difference between the two strand, to verify the guess, we using the technology of bioinformatics, compare the base usage between the DNA double strand in 17 species bacteria. The result show: 1. there is same bases usage frequency in coding sequence between leading strand and lagging strand 2. There also same bases usage frequency in first codon, second codon and third codon. It suggest that there is a equilibrium between the two strand by the effect of select pressure and nature mutation.

Bacteria↗

[Enhanced sensitivity of ovarian cell line to cisplatin induced by mifepristone and its mechanism].

OBJECTIVE: To observe the effect of mifepristone in enhancing chemosensitivity of drug-resistant ovarian cancer cell line to cisplatin and investigate its mechanism. METHODS: Human ovarian cancer cell lines COC1 (DDP-sensitive) and COC1/DDP (DDP-resistant) were incubated with or without mifepristone. The proliferation rate of the cells was determined by methyl thiazolyl tetrazolium (MTT) assay and positive expression of apoptosis-associated proteins Bcl-2 and Bax were observed by means of flow cytometry. RESULTS: It was observed that mifepristone at the dose of 1.25 micromol/L reversed the resistance of COC1/DDP cells to cisplatin, and Bcl-2 protein expression was deceased from (23.8067+/-0.4382)% to (19.3967+/-0.6866)% (P=0.000) while Bax protein expression increased from (12.75+/-0.2524)% to (25.5967+/-0.8834)% (P=0.000). CONCLUSION: Mifepristone may act to enhance the sensitivity of COC1/DDP cells to cisplatin, possibly through regulating Bcl-2 and Bax protein expressions.

Abortifacient Agents, Steroidal↗