Biomedical subjects

Tang-bin Yang

Publications and source records attributed to Tang-bin Yang.

5 recordsLinked to original sources

PubMed2005

[Detection of small molecules based on surface plasmon (correction of plasman) resonance technology].

OBJECTIVE: To develop a detective method applied in online assaying of astronauts' humours using the portable online bio-molecules analyzer (POBA) based on surface plasmon resonance biosensor. METHOD: An assay format was developed based on the detection of 2, 4-Dinitrophenyl-hydrazine. The bio-molecule slide was made by DNP-BSA. Range of detection and standard curve were obtained using inhibition assay. Reliability and specificity of the assay were also tested. RESULT: 1) The linear range of the assay was 7.8 ng/ml-2 micrograms/ml with lower detection limit of 2.5 ng/ml; 2) Preparation of the bio-molecule slide and regeneration of the biosensor ensured detections for many samples. CONCLUSION: This assay method can be used to detect small molecules sensitively, rapidly and easily. It can be repeated with good reliability, and has a good application in space medicine.

Aerospace Medicine↗

PubMed2005

[Preparation of polyclonal and monoclonal antibodies against CRF and change of CRF in the sleep-deprived rat brain].

AIM: To produce polyclonal and monoclonal antibodies against corticotropin-releasing factor (CRF) and to study the changes of CRF in sleep-deprived rat brain with the antibodies acquired. METHODS: Commercial CRF was linked to bovine throglobulin (BTG) and bovine serum albumin (BSA) respectively to produce immunogen and embedding antigen. New Zealand rabbits and BALB/c mice were immunized with the BTG-CRF immunogen to produce polyclonal and monoclonal antibodies, respectively. The acquired antibodies were appraised with ELISA and immnohistochemical staining. The characterized antibodies were used to observe the changes of CRF in the rat brain 48 h after sleep deprivation. RESULTS: CRF polyclonal antibody and 9 clones of monoclonal antibodies were obtained with high titer, affinity and specificity. Among them, the polyclonal antibody and 2 monoclonal antibodies (1D10, 2F4) were excellent in immunocytochemical staining. The CRF-like immunoreactive substances were found more strongly expressed in the neurons of paraverticular hypothalamic nucleus (PVN), central subnucleus of amygdala (CeA), oval subnucleus of bed nucleus of stria terminalis (BNSTov), and nucleus of solitary tract (NTS) in sleep-deprived rat brain. While they were much weaker and even absent in the control. CONCLUSION: The polyclonal and monoclonal antibodies against CRF were successfully produced for immunocytochemical studies. The results indicate that CRF may play an important role in stress-responsive modulation during sleep deprivation. PVN, CeA and BNSTov are integral part of brain circuit related to the stress modulation.

Animals↗

PubMed2003

[Changes of endocrine hormones during -6 degrees head down bed rest in human].

OBJECTIVE: To investigate changes of endocrine hormones during 7 d head down bed rest (HDBR). METHOD: Six healthy male volunteers served as the subjects and experienced 7 d -6 degrees HDBR. Urine was collected from 6:00-22:00 and from 22:00-6:00. Serum was collected 48 h before HDBR, 48 h and 120 h during HDBR. Then the endocrine indices in urine and serum were determined. RESULT: 1) The levels of serum CORT and ALD increased at 48 h during HDBR, while serum T3, T4, TP, UN decreased but they all recovered to normal at 120 h during HDBR. 2) The level of urine CORT, ALD and NE reached its peak in 24-48 h, and then gradually decreased to normal level. CONCLUSION: The endocrine indices in serum and urine changed in the early period but returned to normal level gradually with the proceeding of HDBR.

Adaptation, Physiological↗

PubMed2003

[Space flight and peroxidative damage].

Space flight is associated with an increase of peroxidative damage after returning to 1 g. The effect is more pronounced after long-duration space flight and can even last for several weeks after landing. In humans there is increased lipid peroxidation in erythrocyte membranes, reduced blood antioxidants, and increased urinary excretion of 8-iso-prostaglandin F2alpha, and 8-oxo-7, 8 dihydro-2 deoxyguanosine. Isoprostane 8-iso-prostaglandin F2alpha and 8-oxo-7, 8 dihydro-2 deoxyguanosine are markers for oxidative damage to lipids and DNA, respectively. The changes are attributed to a combination of energy deficiency that occurs during flight and substrate competition for amino acids occurring between repleted muscle and other tissues during the recovery phase. The observations in humans have been complemented by studies in rodents, which showed increased production of lipid peroxidation products and decreased antioxidant enzyme activity afterflight. The changes in rodents were attributed to the stress associated with re-entry into Earth's gravity. Reducing the imbalance between the production of endogenous oxidant defenses and oxidant production by increasing the supply antioxidants in diet may lessen the severity of the postflight increase in oxidative stress.

Animals↗

PubMed2002

[Advances in monoclonal antibody researches].

Monoclonal antibody techniques are very important tools in modern life science research. Despite extensive research efforts paid in recent years, and promising results yielded in the study on the structure and function of genes and proteins, there is still a great need for further researches on the definition, principle and applicability of some immunological methods. This review gives an overview of the advances in immunological researches, including DNA immunization, cellular immunization and preparation of monoclonal antibodies. Using methods of modem molecular immunology, such as genetic immunization, cellular immunization, subtractive immunization and repetitive immunization multiple sites (RIMMS), to construct eukaryotic expression vector and to prepare high-affinity monoclonal antibodies in short time, the conventional method which is time-consuming and laborious could be improved. It is meaningful to the field of basic research and application, such as proteomics, biochip, clinical medicine and diagnosis and therapy of diseases.

Aerospace Medicine↗