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Thomas Magee

Publications and source records attributed to Thomas Magee.

15 recordsLinked to original sources
PubMed2006

Phosphodiesterase type 5 is not upregulated by tadalafil in cultures of human penile cells.

OBJECTIVE: Tadalafil, a long-acting phosphodiesterase type 5 (PDE5) inhibitor, improves the erectile response by inhibiting cyclic guanosine monophosphate (cGMP) breakdown. Sustained higher levels of cGMP may hypothetically upregulate PDE5 expression and/or activity and lead to tachyphylaxis. We have investigated whether PDE5 upregulation occurs in vitro in cultures of human penile cells subjected to long-term incubation with increasing concentrations of tadalafil in the presence of a nitric oxide (NO) donor. METHODS: Human corpora cavernosa smooth muscle cells (CSMC) and tunica albuginea fibroblasts (TAF) primary cultures were characterized by immunocytochemistry and Western blot, and incubated with graded concentrations of tadalafil for 14 days, adding S-nitroso-N-acetyl penicillamine (SNAP) as an NO donor for the last 24 hours or at time zero, and cGMP levels were measured. Incubations were repeated for 7, 10, and 14 days, in the presence of SNAP, and PDE5 was estimated by Western blot, and at 14 days, by immunocytochemistry combined with quantitative image analysis, and by real-time reverse transcriptase polymerase chain reaction (RT-PCR). Constructs of the human PDE5A promoter expressing luciferase were cloned and transfected into CSMC, and promoter activation by 8-deoxybromo-cGMP (8-Br-cGMP) was measured by luminometry. RESULTS: Incubations of CSMC with SNAP and tadalafil up to 14 days did not upregulate PDE5 mRNA or protein levels. With TAF, PDE5 protein was also not upregulated despite a slight increase in mRNA levels. PDE5 enzyme activity was unaffected by tadalafil in either CSMC or TAF. No upregulation of the PDE5 promoter was observed with up to 2 mM 8-Br-cGMP. CONCLUSIONS: Long-term incubation of human penile cells with tadalafil at concentrations above the in vitro IC50, and around the in vivo Cmax utilized in the clinical setting, did not upregulate PDE5A expression nor decrease cGMP levels. These data suggest that PDE5 upregulation is unlikely to occur in vivo on long-term tadalafil treatment.

3',5'-Cyclic-GMP Phosphodiesterases↗
PubMed2006

3.0-T MRI of meniscal tears.

OBJECTIVE: The sensitivity and specificity of 3.0-T MRI of the knee for meniscal tears have not been specifically assessed. We undertook a retrospective review of 100 consecutive MR examinations of the knee followed by arthroscopy to evaluate the sensitivity and specificity of 3.0-T MRI compared with arthroscopy in the detection of meniscal tears. MATERIALS AND METHODS: Two experienced musculoskeletal radiologists retrospectively reviewed MR scans of the knees of 100 patients who underwent consecutive knee arthroscopic procedures after MRI examinations. Interpretations were performed by consensus review with the reviewers blinded to arthroscopic results at the time of consensus review. All patients underwent complete MRI of the knee on a 3.0-T unit. All patients underwent fast spin-echo proton density sagittal imaging at 2-mm slice thickness. Fat-saturated fast spin-echo T2 axial, coronal, and sagittal imaging and T1 coronal imaging also were performed. All 100 patients underwent arthroscopy, and the results were compared with MR interpretations. RESULTS: Consensus retrospective review of the 100 knee MR examinations revealed 111 meniscal tears. Four meniscal tears seen on arthroscopy were not seen on MR examination. There were three false-positive MR interpretations of meniscal tear compared with arthroscopy. In this study, the sensitivity of MRI in the detection of meniscal tears was 96%, and the specificity was 97%. CONCLUSION: MRI of the knee at 3.0 T is sensitive and specific compared with arthroscopy in the detection of meniscal tears. Findings at 3.0 T compare favorably with results at 1.5-T or lower field strength.

Adolescent↗
PubMed2006

3.0-T MRI of the supraspinatus tendon.

OBJECTIVE: MRI of the shoulder has been found to be highly sensitive and specific for detection of full-thickness supraspinatus tendon tears at 1.5-T or lower field strength compared with arthroscopy. MRI of the shoulder at 3.0 T has not been specifically assessed. This study assesses the sensitivity and specificity of MRI at 3.0 T for supraspinatus tendon tears compared with arthroscopy. MATERIALS AND METHODS: Two experienced musculoskeletal radiologists retrospectively reviewed MR images of the shoulder in 150 consecutive patients who had subsequent arthroscopy. All patients had oblique coronal and sagittal T1-weighted and fat-saturated T2-weighted axial, oblique coronal, and sagittal imaging performed. The radiologists interpreted the MR images by consensus without knowledge of the arthroscopy results. Scans were interpreted for full-thickness or partial-thickness supraspinatus tendon tears. If partial-thickness supraspinatus tendon tears were seen on MRI, the reviewers noted whether the partial-thickness tear was articular or bursal in location. The radiologists also separated the supraspinatus tendon tears into small (< 1 cm retraction from the humeral head) and large (> 1 cm retraction from the humeral head). All 150 patients went on to arthroscopy. After consensus review of the MR images, arthroscopy results were compared with consensus MR interpretations. RESULTS: Ninety-eight of the 150 patients had full-thickness supraspinatus tendon tears at arthroscopy. Twenty-six of the 150 patients had partial-thickness supraspinatus tendon tears. Seventeen of these 26 partial-thickness tears were along the articular surface and nine were along the bursal surface. Ninety-six of 98 full-thickness tears seen at arthroscopy were seen on consensus MRI interpretation. All 26 partial-thickness tears seen at arthroscopy were seen at consensus MR interpretation; however, two of the partial-thickness articular surface tears seen at arthroscopy were interpreted as full-thickness tears on consensus MRI interpretation. Twenty-eight of the 98 full-thickness supraspinatus tendon tears were small tears (< 1 cm retraction from the humeral head) on arthroscopy. Two of these 28 small tears seen on arthroscopy were not seen on consensus MRI interpretation. Twenty-six patients had intact supraspinatus tendons on both retrospective consensus MRI interpretation and at arthroscopy. CONCLUSION: MRI of the shoulder at 3.0 T is highly sensitive and specific compared with arthroscopy in the detection of full-thickness and partial-thickness supraspinatus tendon tears.

Adolescent↗
PubMed2004

Prevalence of meniscal radial tears of the knee revealed by MRI after surgery.

OBJECTIVE: Meniscal resection decreases the ability of the meniscus to evenly distribute forces placed on it. These forces are oriented centrifugally on the meniscus by normal weight-bearing and are distributed by circumferentially oriented fibers. This alteration may predispose the knee to radial tears after surgery. SUBJECTS AND METHODS: One of three musculoskeletal radiologists prospectively interpreted 100 consecutive postoperative MRI examinations of the knee. A prospective MRI report was generated for the referring orthopedic surgeon, and prospective MRI interpretations were correlated with arthroscopic findings (n = 63). MRI examinations on those patients who underwent second-look arthroscopy were retrospectively reviewed by three musculoskeletal radiologists who reached a consensus on the prevalence of new postoperative meniscal radial tears. MRI criteria for radial tear diagnosis were used as outlined by Tuckman et al.: truncation, abnormal morphology, lack of continuity, absence of the meniscus, or any combination of those criteria on one or more MR images. An additional criterion used was abnormal increased signal in that area on T2-weighted images. RESULTS: Thirty-two of the 100 patients had meniscal radial tears on prospective MRI interpretations. In 29 of these 32 patients, second-look arthroscopy confirmed meniscal radial tears in the areas described on the MRI examinations. Five additional radial tears were shown on second-look arthroscopy that were not seen on prospective MRI interpretations. Two of those additional five radial tears were seen on consensus retrospective MRI review. CONCLUSION: In this study, a 32% prevalence of meniscal radial tears in the postoperative knee was present on prospective MRI interpretations as opposed to a reported 14% prevalence in the nonoperated knee. Meniscal resection decreases the ability of the meniscus to evenly distribute forces placed on it. This circumstance may increase the prevalence of meniscal radial tears in the postoperative knee. New meniscal radial tears are common in patients presenting with pain after knee surgery.

Adolescent↗
PubMed2004

Usefulness of simultaneous acquisition of spatial harmonics technique for MRI of the knee.

OBJECTIVE: Simultaneous acquisition of spatial harmonics (SMASH) is a parallel-imaging technique that uses fewer echoes than conventional techniques to obtain the desired resolution. Images are produced in a shorter time period using the SMASH technique than conventional techniques. This study assesses the usefulness of the SMASH technique in the MRI evaluation of the knee. SUBJECTS AND METHODS. Three experienced musculoskeletal radiologists prospectively interpreted MR images of the knee in 50 consecutive patients. All patients underwent a complete MRI examination of the knee on a 1.5-T MRI scanner. Sagittal proton density fat-saturated and coronal T1-weighted images were obtained. In addition, fat-saturated T2-weighted images were obtained in the coronal, sagittal, and axial planes. SMASH T2-weighted imaging in the coronal, axial and sagittal planes took 6 min 38 sec, whereas fast spin-echo conventional T2-weighted imaging took 11 min 45 sec to obtain images in the same planes. Each radiologist interpreted the knee MR examinations prospectively and was unaware whether the images had been obtained using the SMASH T2-weighted or fat-saturated T2-weighted technique. The radiologists provided a report. The three radiologists independently performed a retrospective review of both the fat-saturated T2-weighted and SMASH T2-weighted images. The radiologists were blinded as to which sequence was fat-saturated T2-weighted and which was SMASH T2-weighted. They were asked to reinterpret the images and determine whether either of the T2-weighted sequences altered the original interpretation. RESULTS: There was no intraobserver variability in MRI interpretations with the use of SMASH imaging as compared with fat-saturated T2-weighted images. Thirty-four patients underwent arthroscopy after imaging. Findings at arthroscopy in those patients were as follows: 28 meniscal tears, 12 anterior cruciate ligament tears, and nine chondral defects. In some patients, multiple abnormalities were detected on MRI. All arthroscopy findings correlated with prospective MRI interpretations. CONCLUSION: The use of SMASH T2-weighted imaging as opposed to fat-saturated T2-weighted imaging results in a significant decrease in imaging time (> 5-min decrease for each knee examination) without affecting the MRI interpretation or patient clinical outcome.

Adult↗
PubMed2004

Shoulder MR arthrography: which patient group benefits most?

OBJECTIVE: We sought to compare the diagnostic accuracy of conventional MRI versus MR arthrography of the shoulder in the assessment of high-performance athletes (professional baseball players) and to compare our findings in these patients with the conventional MRI and MR arthrographic findings in an age-matched control group of nonprofessional athletes. MATERIALS AND METHODS: Conventional MRI and MR arthrographic examinations of the shoulder in 20 consecutive professional baseball players with shoulder pain were reviewed retrospectively by two musculoskeletal radiologists in consensus. These interpretations were compared with retrospective consensus interpretations of conventional MRI and MR arthrographic examinations of the shoulder obtained in a control group of 50 consecutive nonprofessional athletes with shoulder pain. MR images were assessed for full- or partial-thickness supraspinatus tendon tears, superior labral anteroposterior (SLAP) tears, and anterior or posterior labral tears. RESULTS: In the 20 consecutive professional athlete patients, two full-thickness and six partial-thickness undersurface supraspinatus tendon tears were seen on MR arthrography but not seen on conventional MRI as well as six SLAP tears, two anterior labral tears, and one posterior labral tear. Three patients had both SLAP tears and full- or partial-thickness supraspinatus tendon tears. Of 14 patients with findings on MR arthrography that were not seen on MRI, 11 had arthroscopic correlation. In all 11, arthroscopic findings confirmed findings on MR arthrography. In the group of 50 nonprofessional athlete patients, five had additional findings on MR arthrography not seen on conventional MRI: two anterior labral tears, two partial-thickness supraspinatus tendon tears, and two SLAP tears. One patient had both a partial-thickness supraspinatus tendon tear and a SLAP tear seen on MR arthrography. The five patients with additional findings on MR arthrography had arthroscopy. In all five, arthroscopic findings confirmed the findings on MR arthrography. CONCLUSION: MR arthrography is considerably more sensitive for detection of partial-thickness supraspinatus tears and labral tears than conventional MRI. MR arthrography showed injuries in addition to those seen on conventional MRI in 14 of 20 patients in the high-performance athlete group. These results suggest high-performance athletes may be a subgroup of patients for whom MR arthrography yields considerably more diagnostic information than conventional MRI.

Adolescent↗
PubMed2004

Detection of meniscal tears and marrow lesions using coronal MRI.

OBJECTIVE: The usefulness of coronal images in addition to sagittal images for detection of additional meniscal injuries or bone lesions has been questioned. Some authors believe meniscal tears are rarely seen only in the coronal plane. We performed a retrospective review of coronal and sagittal MR images of the knee to determine whether coronal imaging resulted in the detection of any additional meniscal tears or bone lesions when compared with sagittal MRI of the knee alone. MATERIALS AND METHODS: Two musculoskeletal radiologists retrospectively reviewed 200 consecutive sets of MR images of the knee by consensus. Both observers recorded their retrospective findings on sagittal proton density images, sagittal and coronal T2-weighted images, and coronal T1-weighted images. Findings recorded were those of meniscal tears, bone marrow abnormalities, and bone lesions. RESULTS: On these 200 consecutive sets of MR images of the knee, 114 meniscal tears were shown. Ninety-three meniscal tears were shown on sagittal proton density images only. One hundred fourteen meniscal tears were seen on sagittal proton density and coronal T1- and T2-weighted images. Use of coronal images resulted in the confident detection of 21 additional meniscal tears not well seen on sagittal proton density images alone. Twelve of the 21 additional meniscal tears were seen on coronal T1- and T2-weighted images. Nine of the 21 additional meniscal tears were seen only on coronal T1-weighted images. Most of these additional meniscal tears were in the body of the meniscus. These 21 additional meniscal tears were confirmed arthroscopically. Eight marrow lesions were well characterized only on coronal T1-weighted images. Five were osteochondral lesions, one was an intraosseous hemangioma, and two were microfractures. CONCLUSION: Coronal MR images of the knee allowed better detection and characterization of some meniscal tears than sagittal images alone. Radial meniscal tears, bucket-handle tears, and horizontal tears in the body of the meniscus may be difficult to characterize in the sagittal plane alone. Use of coronal T1-weighted images, rather than coronal T2-weighted or sagittal proton density images alone, allows accurate characterization of some additional marrow lesions. The addition of a T1-weighted sequence in the coronal plane adds only 1 min 30 sec to the scanning time.

Adolescent↗
PubMed2003

MR arthrography of postoperative knee: for which patients is it useful?

PURPOSE: To assess which postoperative patients benefit most from MR arthrography of the knee. MATERIALS AND METHODS: One hundred consecutive MR arthrograms obtained in patients who had previous knee surgery underwent retrospective review in consensus by three radiologists after prospective reading by one of the three radiologists. Criterion on MR arthrograms for a retear was abnormal tracking of an intraarticular dilute gadolinium-based contrast material and saline mixture into the substance of a meniscus. Patients were separated into three groups: those with more than 25% meniscal resection, those with less than 25% meniscal resection, and those with meniscal repair. All 100 patients had preoperative MR images to review directly in conjunction with the postoperative MR images. Fifty-seven of these 100 patients underwent second-look arthroscopy. RESULTS: Nine patients had MR findings consistent with avascular necrosis. Nineteen patients had marked degenerative arthrosis in the area of previous surgery. Seven patients had chondral defects or injuries. Twenty-nine patients had clear MR evidence of a meniscal retear without any contrast material injected into the joint. In 32 of the 100 patients, intraarticular contrast material was useful in demonstrating a retear. Of these 32 patients, 22 had MR arthrographic evidence of a retear, while 10 had no clear MR arthrographic finding to explain postoperative pain. Four additional patients had no clear MR imaging or MR arthrographic abnormality. All patients with meniscal repair (n = 16) needed MR arthrography to diagnose a residual or recurrent meniscal tear. No patient with less than 25% meniscal resection (n = 23) needed MR arthrography to demonstrate a residual or recurrent meniscal tear. Sixteen of 61 patients with more than 25% meniscal resection needed MR arthrography to demonstrate a residual or recurrent meniscal tear. CONCLUSION: All patients with meniscal repair required MR arthrography. All patients with meniscal resection of more than 25%, who did not have severe degenerative arthrosis, chondral injuries, or avascular necrosis required MR arthrography. Patients with less than 25% meniscal resection did not need MR arthrography.

Adolescent↗
PubMed2003

Usefulness of the simultaneous acquisition of spatial harmonics technique during MRI of the shoulder.

OBJECTIVE: The simultaneous acquisition of spatial harmonics (SMASH) technique is a parallel imaging technique that uses fewer echoes than conventional techniques to obtain the desired resolution. Production of images occurs more quickly with parallel imaging than with conventional imaging. This study assesses the usefulness of the SMASH technique for MRI of the shoulder. SUBJECTS AND METHODS. Five experienced musculoskeletal radiologists prospectively interpreted MRIs of the shoulder in 50 consecutive patients. All patients underwent a complete MRI examination of the shoulder on a 1.5-T commercially available scanner. Axial fat-saturated proton density-weighted, coronal T1-weighted, and coronal and sagittal fat-saturated T2-weighted sequences were performed. Patients also underwent SMASH T2-weighted imaging in the coronal and sagittal planes. Coronal and sagittal SMASH T2-weighted imaging took approximately 50% as long to complete compared with conventional T2-weighted imaging. Each radiologist interpreted MRIs of the shoulder without knowing whether the images were obtained using the SMASH or the fat-saturated T2-weighted technique. Twenty-eight of the 50 patients also underwent subsequent arthroscopy, and the results were compared with MRI interpretations based on SMASH and fat-saturated T2-weighted images. RESULTS: We found no intraobserver variability between the interpretations of SMASH imaging and the interpretations of fat-saturated T2-weighted imaging. Of the 28 patients who also underwent subsequent arthroscopy, 19 were found to have full-thickness supraspinatus tendon tears and nine had superior labral anterior-to-posterior lesions. All arthroscopic findings correlated with prospective MRI interpretations. CONCLUSION: SMASH imaging allows significant time savings compared with fat-saturated T2-weighted MRI. We found that the use of the SMASH technique did not affect the MRI interpretation or the clinical outcome of patients.

Adult↗
PubMed2003

Comparison of high-field-strength versus low-field-strength MRI of the shoulder.

OBJECTIVE: Previous studies have reported similar results of shoulder MRI versus arthroscopy for high-field-strength (1.5-T) and low-field-strength (0.2-T) units. We report our experience with the accuracy of high- versus low-field-strength units versus arthroscopy for detection of supraspinatus tendon tears and labral tears in the same patients. SUBJECTS AND METHODS. Three musculoskeletal radiologists prospectively interpreted shoulder MRIs from 40 patients who had a complete shoulder MRI examination on a 0.2-T system and limited imaging on a 1.5-T unit. Proton-density axial and fat-saturated T2-weighted coronal and sagittal sequences were performed. Each radiologist interpreted the open unit images first and the high-field-strength images second. Results from 28 patients who also underwent arthroscopy were also compared with the MRI interpretations. All scans were then retrospectively reinterpreted by consensus of the three reviewers, who were unaware of the patient's name, results of previous MRI, or arthroscopy report (if any). RESULTS: High-field-strength images altered reviewers' interpretations of low-field-strength scans for nine of 40 patients. In four patients, full-thickness supraspinatus tendon tears could be diagnosed definitively on the high-field-strength unit but not on the open unit. Three labral tears and two superior labral anteroposterior lesions could be depicted definitively on the high-field-strength unit but not on the open unit. All tears were confirmed at arthroscopy. CONCLUSION: High-field-strength MRI units provide better spatial and contrast resolution and allow more accurate interpretations than low-field-strength units; these findings may affect clinical treatment.

Arthroscopy↗
PubMed2003

Complications of rotator cuff surgery in which bioabsorbable anchors are used.

OBJECTIVE: This study assessed the utility of MRI in patients with new or persistent pain after surgery with bioabsorbable rotator cuff anchors. SUBJECTS AND METHODS. Three musculoskeletal radiologists prospectively reviewed MRIs of 30 patients with pain after rotator cuff repair with fixation by rotator cuff anchors. Each radiologist described the location of the rotator cuff anchors and whether the supraspinatus tendon was intact or not. MRI findings were correlated with second-look arthroscopy. Consensus MRI interpretations by the three radiologists were obtained retrospectively. RESULTS: Of the 30 patients, nine had dislodgement of the rotator cuff anchors from the humeral head along with a full-thickness supraspinatus tendon retear. The dislodged rotator cuff anchor position could be determined on coronal and sagittal MRIs, providing the orthopedic surgeon a preoperative map for tendon reattachment and retrieval of the dislodged rotator cuff anchor. Four patients had loose rotator cuff anchors but intact supraspinatus tendons. Three patients had supraspinatus tendon retears, but the rotator cuff anchors were intact. In all 16 patients, arthroscopic findings confirmed MRI findings. Fourteen patients had intact rotator cuff anchors and intact supraspinatus tendons on MRI. Of these 14 patients, five had second-look arthroscopy confirming MRI findings. CONCLUSION: MRI is useful in the assessment of patients with persistent or new-onset pain after supraspinatus tendon repair with rotator cuff anchors. MRI provides a presurgical map for second-look arthroscopy to assess retear of the supraspinatus tendon and also aids in retrieval of dislodged rotator cuff anchors.

Absorbable Implants↗
PubMed2002

MR accuracy and arthroscopic incidence of meniscal radial tears.

OBJECTIVE: A meniscal radial tear is a vertical tear that involves the inner meniscal margin. The tear is most frequent in the middle third of the lateral meniscus and may extend outward in any direction. We report (1) the arthroscopic incidence of radial tears, (2) MR signs that aid in the detection of radial tears and (3) our prospective accuracy in detection of radial tears. DESIGN AND PATIENTS: Three musculoskeletal radiologists prospectively read 200 consecutive MR examinations of the knee that went on to arthroscopy by one orthopedic surgeon. MR images were assessed for location and MR characteristics of radial tears. MR criteria used for diagnosis of a radial tear were those outlined by Tuckman et al.: truncation, abnormal morphology and/or lack of continuity or absence of the meniscus on one or more MR images. An additional criterion used was abnormal increased signal in that area on fat-saturated proton density or T2-weighted coronal and sagittal images. Prospective MR readings were correlated with the arthroscopic findings. RESULTS: Of the 200 consecutive knee arthroscopies, 28 patients had radial tears reported arthroscopically (14% incidence). MR readings prospectively demonstrated 19 of the 28 radial tears (68% sensitivity) when the criteria for diagnosis of a radial tear were truncation or abnormal morphology of the meniscus. With the use of the additional criterion of increased signal in the area of abnormal morphology on fat-saturated T2-weighted or proton density weighted sequences, the prospective sensitivity was 25 of 28 radial tears (89% sensitivity). There were no radial tears described in MR reports that were not demonstrated on arthroscopy (i.e., there were no false positive MR readings of radial tears in these 200 patients). CONCLUSIONS: Radial tears are commonly seen at arthroscopy. There was a 14% incidence in this series of 200 patients who underwent arthroscopy. Prospective detection of radial tears was 68% as compared with arthroscopy when the criteria as outlined by Tuckman et al. were used alone. With the use of the additional criterion of increased signal in the area of abnormal morphology on fat-saturated T2-weighted and proton density weighted sequences, the prospective sensitivity for radial tear detection as compared with arthroscopy was 89% in our series. Fat-saturated proton density and T2-weighted images greatly improve the detection of radial tears as signal intensity changes in radial tears as well as morphologic changes can be utilized for the detection of subtle tears.

Adolescent↗
PubMed2002

Skeletal muscle metastases at sites of documented trauma.

OBJECTIVE: Hematogenous metastases to skeletal muscles have been reported to be rare. We report eight biopsy-proven cases of skeletal metastases occurring in sites of previously documented skeletal muscle trauma. We retrospectively reviewed MR imaging examinations obtained at a large orthopedic surgical oncology service from January 1994 through December 2000 for biopsy-proven metastases to skeletal muscles. Our retrospective review revealed 28 patients with biopsy-proven skeletal muscle metastases. Of these 28 patients, eight had a documented clinical history of previous trauma at the site of skeletal metastasis. Five of these eight patients underwent MR imaging before the development of a metastasis. MR imaging revealed a hematoma in three of the five patients and a partial muscle tear in two of the five patients. The hematomas and partial muscle tears were in the same skeletal muscle location in which the metastatic disease subsequently developed. Metastatic disease was documented by MR imaging and subsequent biopsy. CONCLUSION: Skeletal muscle injury may alter muscle physiology and result in increased susceptibility to the development of metastatic disease at such sites.

Adult↗
PubMed2002

Future strategies for treating erectile dysfunction.

Erectile dysfunction affects over half of all men between 50 and 70 years of age, and by the age of 40, about 40% of men may suffer from some form of erectile dysfunction. Many disease states, such as diabetes, hypertension, depression, and vascular disease, are associated with the condition, which may occur many years prior to the onset of these disorders. The phenomenal success of sildenafil in improving erections in men with erectile dysfunction is due to the fact that the drug, as a phosphodiesterase inhibitor, improves the relaxation of smooth muscle cells, which become dysfunctional with the aging process. However, not everyone responds to this medication, mainly because the efficacy of the drug is directly dependent on the release of nitric oxide from the nerve terminals of the cavernosal nerve, and this may become defective with aging/certain disease states. The goal of gene therapy for organic impotence is to allow the patient to sustain physiologically elicited erections without resorting to pharmacological treatment immediately prior to the sexual act. Experimental efforts in gene therapy for erectile dysfunction are likely to continue intensively in a series of directions, some specific to the nature of the selected gene to be manipulated or the physiology of the corpora cavernosa itself, and others extrapolatable from the advancement of gene therapy in general.

Journal Article↗