Biomedical subjects

Tian-qiu Mao

Publications and source records attributed to Tian-qiu Mao.

4 recordsLinked to original sources

PubMed2006

[Repair of peripheral nerve defect by a scroll of amnion derivative compound with cultured autogenous Schwann cell in a rat model].

OBJECTIVE: To test a nerve bridge substitute for peripheral nerve repair by tissue-engineering approach. METHODS: An artificial nerve fabricated with a scroll of amnion derivative (ZQ membrane) and cultured autogenous Schwann cell was sutured to bridge sciatic nerve defect of 2.5 cm in length in rats. The specimens were assessed with tracking study, histology, electrophysiological technique, NF200, and synaptophysin-38 (SYP) immuno histochemical staining 3 months postoperatively. RESULTS: The regenerated nerve sprouted 3 months after the operation. The regenerated nerve fibers were plentiful and could grow into the recipient nerve and target muscle's motor end plate (MEP) areas to reinnervate target muscle, and reconstruct function of nerve-muscle junction. Functional recovery could reach to 40%-60% of normal control. Nerve-muscle conduction velocity (N-MCV) arrived at 21.77 +/- 1.15 m/s. CONCLUSIONS: A tissue engineering material fabricated with a scroll of ZQ membrane and cultured autologous Schwann cell may be a useful substitute for nerve repair.

Amnion↗

PubMed2004

[Surgical orthodontic technique for the treatment of maxillofacial deformities and dysfunction of occlusion after maxillofacial fractures].

OBJECTIVE: To evaluate a new technique to treat severe maxillofacial deformity and dysfunction of occlusion after the maxillofacial fractures. METHODS: Thirty-four consecutive patients, with delayed maxillofacial deformities and dysfunction of occlusion after the maxillofacial fractures, were treated by the use of x-ray cephalometric analysis, model surgery, open reduction and rigid internal fixation. RESULTS: Thirty-three patients were successfully corrected the maxillofacial deformities, facilitated normal occlusal relationship. Only one patient with severe damage of the brain was presented a mild occlusion dysfunction one year after the operation. CONCLUSIONS: The above-mentioned technique may be a viable and effective option for the management of the deformities of the face and dentition after the maxillofacial fractures.

Adult↗

PubMed2003

[A study of injectable autogenous tissue-engineered bone].

OBJECTIVE: To investigate the utilization of carrier for delivering osteoblasts and creating autogenous bone tissue in ectopic site of animal via injection. METHODS: Bone marrow cells harvested from iliac bone of New Zealand rabbits were induced to differentiate into marrow stromal osteoblasts. The osteoblasts were mixed with 1.5% alginate sodium solution to generate osteoblasts/alginate composites with final cellular density of 4 x 10(9)/L. Calcium chloride was used as cross-linking agent to gel aqueous alginate solution. The marrow stromal osteoblasts/alginate composites were injected into the dorsal subcutaneous tissue of rabbits with autogenous cells transplantation. The samples were examined with X-ray and histological analysis. RESULTS: Four, eight and twelve weeks after injection, the hard knobbles were easily palpated under the dorsal skin of animals. On X-ray photograph the samples showed calcified image with more density than surrounding soft tissue, new bone formation was observed in the osteoblasts/alginate composites in histological analysis. The osteogenesis was in association with regenerated hematopoietic bone marrow. CONCLUSIONS: These results demonstrate that new bone tissue could be created through the injection of alginate sodium treated with autogenous marrow stromal osteoblasts.

Alginates↗

PubMed2003

[Transforming the sintered ostrich cancellous bone to multiphasic calcium phosphate ceramic].

OBJECTIVE: To transform the sintered ostrich cancellous bone(ostrich true bone ceramic, OTBC) to multiphasic calcium phosphate ceramic and study its components and characterization. METHODS: The OTBC blocks were soaked in different concentration of sodium pyrophosphate (Na(4)P(2)O(7).H(2)O,NP) solution and heated to 1100 degrees C to transform its constitution from HAP into multiphasic calcium phosphate ceramics. Then the surface configuration of the material was observed by SEM and the following properties of the material:porosity,bending strength,mineral composition and element ratio were analyzed. RESULTS: The prepared material was a kind of porous calcium phosphate ceramic composed of beta-TCP, HAP and NaCaPO(4). With increase of the NP concentration,the content of HAP decreased while beta-TCP and NaCaPO(4) increased. The average bending strength of the material was (1.95+/-0.46)Mpa,the Ca/P element ratio was 1.511 and the average porosity was(60.71+/-6.9)%. The pore size was uneven. CONCLUSION: The OTBC could be transformed into beta-TCP /HAP /NaCaPO(4) multiphasic calcium phosphate ceramic by heating with NP. With characteristic porous structure, the prepared OTBC could become a new type of bone graft.

Animals↗