PubMed Health⌕ Search

Biomedical subjects

Tobias Bauer

Publications and source records attributed to Tobias Bauer.

5 recordsLinked to original sources

Iterative full-bandwidth wavefront reconstruction from a set of low-tilt Fizeau interferograms for high-numerical-aperture surface characterization.

Full-bandwidth phase-shifting methods as well as band-limited fringe carrier techniques are both problematic when testing high-NA spherical surfaces in Fizeau interferometers. Phase stepping is usually performed by moving a sample and reference sphere relative to each other along the optical axis. At a high NA the method suffers from phase-shift inhomogeneity across the sample surface. Fringe carrier techniques rely on a minimum fringe frequency and call for an off-axis position of the sample, which in turn introduces condenser aberrations. Distortion of the imaging optics generates further apparent aberrations. We propose to combine both principles. The phase shifts are replaced by a set of very low tilts such that the sample is virtually on axis. Initial wavefront estimates are generated by a fringe carrier method. An adaptive Misell-type algorithm combines the interferometric data and iteratively improves the reconstructed wavefront until full spatial bandwidth is achieved.

Journal Article↗

Polarity of the mouse embryo is established at blastocyst and is not prepatterned.

Polarity formation in mammalian preimplantation embryos has long been a subject of controversy. Mammalian embryos are highly regulative, which has led to the conclusion that polarity specification does not exist until the blastocyst stage; however, some recent reports have now suggested polarity predetermination in the egg. Our recent time-lapse recordings have demonstrated that the first cleavage plane is not predetermined in the mouse egg. Here we show that, in contrast to previous claims, two-cell blastomeres do not differ and their precise future contribution to the inner cell mass and/or the trophectoderm cannot be anticipated. Thus, all evidence so far strongly suggests the absence of predetermined axes in the mouse egg. We observe that the ellipsoidal zona pellucida exerts mechanical pressure and space constraints as the coalescing multiple cavities are restricted to one end of the long axis of the blastocyst. We propose that these mechanical cues, in conjunction with the epithelial seal in the outer cell layer, lead to specification of the embryonic-abembryonic axis, thus establishing first polarity in the mouse embryo.

Animals↗

All-optoelectronic continuous wave THz imaging for biomedical applications.

We present an all-optoelectronic THz imaging system for ex vivo biomedical applications based on photomixing of two continuous-wave laser beams using photoconductive antennas. The application of hyperboloidal lenses is discussed. They allow for f-numbers less than 1/2 permitting better focusing and higher spatial resolution compared to off-axis paraboloidal mirrors whose f-numbers for practical reasons must be larger than 1/2. For a specific histological sample, an analysis of image noise is discussed.

Animals↗

Visualization and classification in biomedical terahertz pulsed imaging.

'Visualization' in imaging is the process of extracting useful information from raw data in such a way that meaningful physical contrasts are developed. 'Classification' is the subsequent process of defining parameter ranges which allow us to identify elements of images such as different tissues or different objects. In this paper, we explore techniques for visualization and classification in terahertz pulsed imaging (TPI) for biomedical applications. For archived (formalin-fixed, alcohol-dehydrated and paraffin-mounted) test samples, we investigate both time- and frequency-domain methods based on bright- and dark-field TPI. Successful tissue classification is demonstrated.

Animals↗

Coherent Hall effect in a semiconductor superlattice.

The coherent Hall effect denotes the transient Hall response of impulsively excited coherent charge-carrier wave packets in a solid. We report the first experimental study of this phenomenon (i) using a semiconductor superlattice in crossed electric and magnetic fields as a model for three-dimensional materials and (ii) employing a contactless optoelectronic technique to probe the transient currents. Two field regimes with distinctly different oscillatory wave packet dynamics are found, separated from each other by a transition region where all oscillations are suppressed.

Journal Article↗