Tomasz S Osiejuk | PubMed Health

PubMed2025

Germline-restricted chromosome of songbirds has different centromere compared to regular chromosomes.

Centromeres are an important part of chromosomes which direct chromosome segregation during cell division. Their modifications can therefore explain the unusual mitotic and meiotic behaviour of certain chromosomes, such as the germline-restricted chromosome (GRC) of songbirds. This chromosome is eliminated from somatic cells during early embryogenesis and later also from male germ cells during spermatogenesis. Although the mechanism of elimination is not yet known, it is possible that it involves a modification of the centromeric sequence on the GRC, resulting in problems with the attachment of this chromosome to the mitotic or meiotic spindle and its lagging during anaphase, which eventually leads to its elimination from the nucleus. However, the repetitive nature and rapid evolution of centromeres make their identification and comparative analysis across species and chromosomes challenging. Here, we used a combination of cytogenetic and genomic approaches to identify the centromeric sequences of two closely related songbird species, the common nightingale (Luscinia megarhynchos) and the thrush nightingale (L. luscinia). We found a 436-bp satellite repeat present in the centromeric regions of all regular chromosomes (i.e., autosomes and sex chromosomes), making it a strong candidate for the centromeric repeat. This centromeric repeat was highly similar between the two nightingale species. Interestingly, hybridization of the probe to this satellite repeat on meiotic spreads suggested that this repeat is missing on the GRC. Our results indicate that the change of the centromeric sequence may underlie the unusual inheritance and programmed DNA elimination of the GRC in songbirds.

Animals↗

PubMed2005

Frequency shift in homologue syllables of the Ortolan Bunting Emberiza hortulana.

Results of this study indicate that in the Ortolan Bunting Emberiza hortulana, syllables of the same shape on sonograms (i.e. homologue syllables) often significantly differ between males in frequency parameters. Typically, homologue syllables of different males in the studied population had a similar bandwidth but shifted minimal and maximal frequencies. We found no strong evidence supporting that the frequency of homologue syllables is affected by the body size variation of males. Besides, frequency parameters of different song types within a male's repertoire did not correlate with each other. Moreover, we found that some males within the population sing two versions of apparently 'the same' song type, which differ in frequency parameters of syllables. Such different frequency versions were sung with eventual variety, which suggests that they are treated as different song types by birds. All these results support the idea that the fine acoustic structure of syllables (and song) is under a strong influence of the acoustic template (i.e. depend mainly on from whom the song was copied). We also found that the frequency of initial syllables within the song strophe is much more variable between males than in case of final syllables. Regardless of the great between-individual variation in initial syllable frequency, the syllables forming the final part of the song are kept within a very restricted bandwidth. These results support our earlier finding that strict syntactic rules of song formation exist in this species. These rules condition the acoustic variation of the initial and final part of the song. At least potentially, such a system enables species recognition and individual variation irrespective of repertoire level. Results of this study indicate that more attention should be paid to the problem of minimal units of song repertoire and to identity of such units within the bird population.

Acoustics↗