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Tong Ren

Publications and source records attributed to Tong Ren.

At least 19 recordsLinked to original sources
PubMed2026

Development and evaluation of a multiplex PCR-based dual-platform targeted sequencing framework for precise differentiation of lumpy skin disease virus.

BACKGROUND: Lumpy skin disease virus (LSDV) shares over 96% genomic identity with goatpox and sheeppox viruses, presenting severe diagnostic challenges due to cross-reactivity. METHODS: To address this bottleneck, we established a targeted sequencing framework integrating multiplex PCR with short-read and long-read platforms. By sequentially screening target pathogens, identifying low-homology genes, and designing short and gradient long-fragment primer pools, we evaluated these dual-platform panels using highly homologous poxvirus samples. RESULTS: The short-read panel stably detected target viruses at inputs as low as 5.26 ×101 copies/μL. Under strict alignment criteria, LSDV mapping rates reached 42.91%, suppressing non-target signals to 3.05%. The Nanopore-Targeted Sequencing (NTS) long-amplicon strategy successfully eliminated homologous interference. By applying length-dependent diagnostic thresholds (≥ 100 reads for short amplicons; ≥ 50 reads for long amplicons), precise species-level identification was achieved, maintaining near-zero cross-reads (0-5) in ultra-long regions. Crucially, the field-deployable NTS workflow enabled complete detection in approximately 4 h. CONCLUSION: This complementary strategy seamlessly meets both laboratory demands for high-sensitivity enrichment and frontline requirements for rapid typing, providing a reliable tool for LSDV surveillance, mutation tracking, and outbreak control.

Capripoxvirus differentiation↗
PubMed2006

Suzuki coupling at the periphery of diruthenium coordination and organometallic compounds.

A series of diruthenium compounds, Ru2(DArF)3(L")Cl (2), where the auxiliary ligand DArF is DmAniF or D(3,5-Cl2Ph)F and L" is one of the diarylformamidinate ligands containing at least one biphenyl, were prepared from Suzuki reactions between Ru2(DArF)3(L')Cl (1), where L' is (4-I-Ph)NC(H)NPh (N-(4-iodophenyl)-N'-phenylformamidinate) or D(4-I-Ph)F (N,N'-di(4-iodophenyl)formamidinate), and ArB(OH)2 (Ar = Ph and 4-CH3C(O)Ph) in satisfactory yields. Alkynylation of the type 2 compounds with LiCCPh yielded the alkynyl derivatives Ru2(DArF)3(L")(CCPh) (3). Alternatively, type 3 compounds can be prepared from the Suzuki coupling reaction between Ru2(DArF)3(L')(C2Ph) and ArB(OH)2. A structural comparison between the type 1 and 2 compounds revealed minimal changes in the coordination sphere of Ru2 core. Cyclic voltammograms of Suzuki derivatives resemble those of the parent compounds, indicating the retention of the electrophore characteristic of diruthenium species upon peripheral modification.

Journal Article↗
PubMed2006

Symmetric and unsymmetric "dumbbells" of Ru2-alkynyl units via C-C bond formation reactions.

Oxidative homocoupling (Glaser) reaction of Ru2 compounds bearing peripheral ethyne resulted in symmetric dimers. Cross-coupling (Sonogashira) reaction between Ru2 compounds bearing peripheral iodo and ethyne groups yielded an unsymmetric dimer. Voltammetric data indicated that Ru2 units in the symmetric dimers are noninteracting, and the unsymmetric dimer is best described as a weakly coupled push-pull compound.

Journal Article↗
PubMed2006

Preparation and characterization of a family of Ru2 compounds bearing iodo/ethynyl substituents on the periphery.

A high-yield synthesis of mixed-bridging-ligand Ru2 compounds, Ru2(D(3,5-Cl2Ph)F)(4-n)(OAc)nCl [n = 1 (1) and 2 (2)] was developed, where D(3,5-Cl2Ph)F is bis(3,5-dichlorophenyl)formamidinate. The acetate ligands in 1 and 2 can be quantitatively displaced with DMBA-I to yield Ru2(D(3,5-Cl2Ph)F)3(DMBA-I)Cl (3) and Ru2(D(3,5-Cl2Ph)F)2(DMBA-I)2Cl (4), respectively, where DMBA-I is N,N'-dimethyl-4-iodobenzamidinate. When compound 2 was treated with 1 equiv of HDMBA-I, a unique Ru2 compound containing three different types of bidentate bridging ligands, cis-Ru2(D(3,5-Cl2Ph)F)2(DMBA-I)(OAc)Cl (5), was obtained. Subsequent reactions between 3/4 and (trimethylsilyl)acetylene under Sonogashira coupling conditions resulted in Ru2(D(3,5-Cl2Ph)F)(4-n)(DMBA-C[triple bond]CSiMe3)nCl [n = 1 (6) and 2 (8)] in excellent yields, which were converted to the corresponding bis(phenylacetylide) compounds Ru2(D(3,5-Cl2Ph)F)(4-n)(DMBA-C[triple bond]CSiMe3)n(C[triple bond]CPh)2 [n = 1 (7) and 2 (9)]. Structural studies of several compounds provided insights about the change in Ru2 coordination geometry upon the displacement of bridging and axial ligands. Voltammetric studies of these compounds revealed rich redox characteristics in all Ru2 compounds reported and a minimal electronic perturbation upon the peripheral Sonogashira modification.

Journal Article↗
PubMed2006

Cu2{[18]ane-N6} complexes: structures, magnetism, and phosphate monoester binding.

A novel Cu(II)2 complex of the [18]ane-N6 macrocycle ([18]ane-N6 = 1,4,7,10,13,16-hexaazacyclooctadecane) was prepared from the reaction between [18]ane-N6 and Cu(II) salts such as Cu(NO3)2 and Cu(OAc)2. A structural study of the complex derived from Cu(OAc)2 (1) revealed a Cu(II)2 core encircled by a [18]ane-N6 ligand and two mu-O-OAc ligands. The facile replacement of mu-O-OAc by a phosphate monoester [PO3(OR)2-] yielded a number of bis(phosphate monoester)dicopper complexes with ROPO3(2-) as hydrogen phosphate (HPO4(2-), 3a), phenyl phosphate [PO3(OPh)2-, 3b], glycerol 2-phosphate [PO3(OCH(CH2OH)2)2-, 3c], alpha-d-gluocose phosphate [PO3(C6H11O6)2-, 3d], and dl-alpha-glycerol phosphate [PO3(OCH2CHOHCH2OH)2-, 3e]. Structural studies of compounds 3a-d confirmed both the retention of the Cu2{[18]ane-N6} core and a mu-O-PO3(OR) coordination mode. Displacement of acetate by a phosphate monoester in an aqueous solution was accompanied by a significant change in the visible absorption, which enables the establishment of relative association constants of PO3(OR)2- on the order of 10(4) in the unbuffered solution and 10(3) in the buffered solution (HEPES). Measurement of the magnetic susceptibility of compound 3a over the temperature range of 5-300 K and subsequent modeling revealed a weak antiferromagnetic coupling (J = -1.1 cm(-1)) between two Cu(II) centers.

Carbon↗
PubMed2005

Structural insights into the coordination and extraction of Pb(II) by disulfonamide ligands derived from o-phenylenediamine.

The o-phenylenediamine-derived disulfonamide ligands 1 and 2 complex and efficiently extract Pb(II) from water into 1,2-dichloroethane via ion-exchange, in combination with 2,2'-bipyridine (97.5% and 95.0%, respectively, for 1:1 ligand-to-Pb ratios). The corresponding Pb(II)-sulfonamido binary complexes of ligands 1 and 2 (3 and 4, respectively), and ternary complexes with 2,2'-bipyridine (5 and 6, respectively), were isolated and characterized. (1)H NMR spectra of the organic phases after extraction show the formation of ternary Pb-sulfonamido-bipy complexes. X-ray characterization of 3, 4, and the ternary complex 5 consistently demonstrates four primary coordination sites and a stereochemically active lone pair on Pb. The X-ray structure of 3 shows a pseudo trigonal bipyramidal configuration on Pb, with the lone pair occupying one of the equatorial sites, and the formation of an unusual "hemidirected" coordination polymer via axial S=O-Pb coordination. The same axial S=O-Pb coordination pattern with two DMSO molecules is observed in the structure of 4.[2(CH(3))(2)SO)], thus rationalizing the high solubility of the binary complexes in strongly coordinating solvents. In contrast, the X-ray structure of the ternary complex 5 reveals a distorted four-coordinate configuration with only weak S=O-Pb coordination leading to dimer formation, thus explaining its higher solubility in weakly coordinating solvents. FT-IR spectroscopy confirms the X-ray data, since the ligand nu(S)(=)(O) stretching frequencies shift to lower values in the binary Pb(II)-sulfonamido complexes and are again altered upon formation of the ternary Pb(II)-sulfonamido-bipy complexes, as would be expected for 2,2'-bipy complexation and hindered S=O-Pb coordination.

Lead↗
PubMed2005

Strong electronic couplings between ferrocenyl centers mediated by bis-ethynyl/butadiynyl diruthenium bridges.

A series of trans-(FcC(2n))Ru(2)(Y-DMBA)(4)(C(2m)Fc) with n, m = 1 and 2 and Y-DMBA as N,N'-dimethylbenzamidinate or N,N'-dimethyl-(3-methoxy)benzamidinate have been synthesized and characterized. The intramolecular Fc...Fc distances, established through single-crystal X-ray diffraction studies, range from 11.6 to 16.6 A. Results from both voltammetric and spectroelectrochemical studies indicate that the (-C(2n))Ru(2)(Y-DMBA)(4)(C(2m-) fragments are among the most efficient mediators of intramolecular hole transfer. Density-functional calculations offer both the insight on the ground-state electronic properties and unambiguous assignment for the observed electronic absorptions.

Crystallography, X-Ray↗
PubMed2005

One-dimensional supramolecular assemblies based on a Re2(III,III) synthon and their solid-state phosphorescence.

Reactions between Re2(DMBA)4(NO3)2 (1; DMBA is dimethylbenzamidinate) and a bidentate dianion, either tungstate (WO4(2-)) or terephthalte (1,4-(O2C)2C6H4(2-)), resulted in the one-dimensional coordination polymers [Re2(DMBA)4(mu-O,O'-WO4)](infinity) (2) and [Re2(DMBA)4(mu-O,O'-1,4-(O2C)2C6H4)](infinity) (3), respectively. Both polymers display phosphorescence from a 3(delta --> delta*) state, which is significantly blue shifted from the phosphorescence previously observed for Re2(DMBA)4Cl2 type compounds.

Journal Article↗
PubMed2005

Proof of large positive zero-field splitting in a Ru2 5+ paddlewheel.

We present the synthesis, as well as the structural and magnetic characterization, of [Ru2(D(3,5-Cl2Ph)F)4Cl(0.5H2O)].C6H14 (D(3,5-Cl2Ph)F = N,N'-di(3,5-dichlorophenyl)formamidinate), a Ru2(5+) compound having a 4B(2u) ground state derived from a sigma2pi4delta2pi2delta electron configuration. The persistence of this configuration from 27 to 300 K is shown by the invariance of the Ru-Ru distance. Orientation-dependent magnetic susceptibility (chiT) and magnetization (M(H)) data are in accord with a spin quartet ground state with large magnetocrystalline anisotropy associated with a large axial zero-field splitting (D) parameter. Theoretical fits to chiT and M(H) plots yielded D/kB = +114 K, implying an S = +/-1/2 Kramers doublet ground state at low temperature. Single-crystal and powder EPR data are consistent with this result, as the only observed transition is between the M(s) = +/1/2 Zeeman levels. The g values are g(perpendicular) = 2.182, g(parallel) = 1.970, and D = 79.8 cm(-1). The totality of the results demands D >> 0.

Crystallography, X-Ray↗
PubMed2005

Diosmium(III) compounds supported by 2-anilinopyridinate and novel alkynyl derivatives.

The reaction between Os(2)(OAc)(4)Cl(2) and Hap (Hap is 2-anilinopyridine) under prolonged refluxing conditions resulted in an Os(III)(2) compound, Os(2)(ap)(4)Cl(2) (1), that can be crystallized as either the cis-(2,2) isomer from a CH(3)OH-CH(2)Cl(2) solution or the (3,1) isomer from a hexanes-CH(2)Cl(2) solution. Compound 1 undergoes facile reactions with LiC(2)Y to yield a series of Os(2)(ap)(4)(C(2)Y)(2) compounds with Y as Ph (2), ferrocenyl (3), SiMe(3) (4), and C(2)SiMe(3) (5). X-ray diffraction study of compound 2 reveals solvent-dependent isomerism similar to that of the parent compound 1. Compound 1 has Os-Os distances of 2.3937(8) and 2.3913(8) Angstroms for the cis-(2,2) and (3,1) isomers, respectively, and is paramagnetic (S = 1). Both the ethynyl derivatives 2-4 and butadiynyl derivative 5 are diamagnetic and have Os-Os distances of 2.456(1), 2.471(1), and 2.481(1) Angstroms for the cis-(2,2) and (3,1) isomers of 2 and (3,1) isomer of 4, respectively. Compounds 1-5 exhibit multiple one-electron redox couples in their cyclic voltammograms, including a reversible Os(2)(8+/7+) couple for 2. Resonance Raman spectra of both compounds 1 and 2 are reported.

Journal Article↗
PubMed2005

Ru2(ap)4(sigma-oligo(phenyleneethynyl)) molecular wires: synthesis and electronic characterization.

Reported in this contribution are the synthesis, characterization, and charge transport properties of wire-like Ru2(ap)4(OPEn), where ap is 2-anilinopyridinate and OPE is -(CCC6H4)nSCH2CH2SiMe3 with n = 1 (1) and 2 (2). Scanning tunneling microscopy (STM) measurements of compound 2 inserted into a SAM of C11 thiol reveal that molecule 2 exhibits (i) the stochastic switching characteristic of wire molecules embedded in insulating SAMs and (ii) higher conductivity than the C11 thiol SAM. More importantly, analysis of the molecular electronic decay constant (beta) exhibits a decrease of at least 15% as compared to purely organic molecular analogues. Hence, the transport characteristics of molecules can be significantly improved for nanoscale electronics through the incorporation of a Ru2 fragment into conjugated backbone.

Journal Article↗
PubMed2005

Fluorescent sensing and selective Pb(II) extraction by a dansylamide ion-exchanger.

The (bis)dansylated sulfonamide 1,2-C6H4(NHSO2C10H6-5-N(CH3)2)2 (1) extracted Pb(II) selectively from water into 1,2-dichloroethane via an ion-exchange mechanism and showed fluorescence quenching upon Pb(II) extraction. The distribution ratios for metal extraction (determined by ICP-MS) for Pb(II) were 133-1410 times higher than those for other metal cations [Co(II), Ni(II), Cu(II), Zn(II), and Cd(II)] under identical conditions. Fluorescence quenching was observed upon Pb(II) extraction, which was dependent on Pb(II) concentration. The monodansylated control, C6H5NHSO2C10H6-5-N(CH3)2 (2), showed neither extraction nor quenching, indicating that the fluorescence effects are a direct result of Pb coordination to 1. The observed selectivity for Pb(II) is ascribed to the formation of a low-coordinate binary Pb(II)-Sulfonamido complex in the organic phase.

Cations, Divalent↗
PubMed2005

[Study on the indication of surgical resection of pulmonary metastasis of malignant trophoblastic tumor].

OBJECTIVE: To investigate the indication of lung lobectomy in patients of malignant trophoblastic tumor with lung metastasis. METHODS: Data on a total of 629 cases of malignant trophoblastic tumor of stage III-IV in Peking Union Medical College Hospital from 1990 to 2003 were reviewed. Ninety-five cases including those that underwent lung lobectomy and cases with normal level of serum human chorionic gonadotropin-beta subunit (beta-hCG, < 2 IU/L) and residual pulmonary nodules after chemotherapy were selected and studied. RESULTS: Lung lobectomies were performed on six cases of invasive mole with lung metastasis and the pathological results were all necrotic nodules;another 35 cases of invasive mole with normal level of serum beta-hCG but residual pulmonary nodules after chemotherapy have been followed up for 6 months to 11 years and all were stable of diseases (SD). Lung lobectomies were performed on 29 cases of choriocarcinoma with lung metastasis. Among them, there were 17 cases whose pathological results were hemorrhage and necrotic tissue without trophoblastic cells (negative pathological results), while trophoblastic cells could still be detected in 12 cases of resected lung specimens (positive pathological results). Twenty-five cases of choriocarcinoma with normal serum beta-hCG but residual pulmonary nodules after chemotherapy were followed up, five cases had progress of disease (PD) and 20 were SD. There were no significant differences of age, stage and the last pregnancy between the positive and negative pathological results, SD and PD groups, respectively. However, the number of chemotherapeutic courses for decreasing beta-hCG from 10 IU/L to 2 IU/L and the total courses in the group of positive pathological results and PD group were significantly more than that of negative pathological results group and SD group (P = 0.01, P = 0.001). CONCLUSIONS: For invasive mole, lung metastasis can be successfully treated by chemotherapy alone. Patients with residual pulmonary nodules but normal serum beta-hCG after chemotherapy can be followed up and spared lung lobectomy. For choriocarcinoma, slowly decreasing of beta-hCG from 10 IU/L to 2 IU/L is a high risk for chemoresistance, and it is an indication for thoracotomy. Progression of disease after multiple chemotherapy courses should be treated with lung lobectomy.

Adult↗
PubMed2004

Sulfide-capped wire-like metallaynes as connectors for Au nanoparticle assemblies.

A new series of Ru2(DMBA)4(oligo(phenyleneethyne))2 compounds bearing sulfide termini was synthesized; structural characterization revealed both the rigid rod nature and extended pi-conjugation in these metallaynes; in the presence of these metallaynes, Au nanoparticles readily assembled into dimers and chains with well-defined inter-dimer distances.

Journal Article↗
PubMed2004

Dirhenium paddlewheel compounds supported by N,N'-dialkylbenzamidinates: synthesis, structures, and photophysical properties.

Dirhenium(III,III) compounds Re2(DMBA)4Cl2 (1, DMBA=N,N'-dimethylbenzamidinate) and Re2(DEBA)4Cl2 (2, DEBA=N,N'-diethylbenzamidinate) were synthesized via molten reactions between Re2(OAc)4Cl2 and the corresponding amidine. Re2(DMBA)4(NO3)2 (3) was obtained through reacting Re2(DMBA)4Cl2 with AgNO3. Single crystal X-ray diffraction studies revealed that the Re-Re distances in compounds 1-3 are 2.212(1), 2.217(1), and 2.173(1) A, respectively, which are consistent with the presence of a Re-Re quadruple bond. Voltammetric studies revealed that compound 2 exhibits two quasireversible couples, an oxidation and a reduction, and an irreversible reduction, while compound 1 displays irreversible couples at similar potentials. The three complexes exhibit 1deltadelta* absorption as a shoulder at approximately 440 nm (epsilon approximately 1500 M(-1) cm(-1)). Upon excitation of solid samples or CH2Cl2 solutions of 2 with visible light, emission is observed at 824 nm (77 K) and 833 nm (298 K), respectively. The luminescence is assigned as arising from the 3deltadelta* excited state.

Benzamides↗
PubMed2003

Synthesis and characterization of Ru2(DMBA)4X2 (X = CN, N3, N(CN)2, I): controlling structural, redox, and magnetic properties with axial ligands.

Metathesis reactions between Ru(2)(DMBA)(4)Cl(2) (DMBA = N,N'-dimethylbenzamidinate) and MX (M = Na and K) yielded bis-adduct derivatives Ru(2)(DMBA)(4)X(2) (X = CN (1), N(3) (2), N(CN)(2) (3)). Metathesis reactions between Ru(2)(DMBA)(4)(NO(3))(2) and KI resulted in Ru(2)(DMBA)(4)I(2) (4). Compound 1 is diamagnetic, while compounds 2-4 are paramagnetic (S = 1). Both compounds 1 and 2 undergo two reversible one-electron processes, an oxidation and a reduction, while compound 3 features a quasireversible reduction. Single-crystal X-ray diffraction studies revealed that the Ru-Ru bond lengths are 2.4508(9), 2.3166(7), 2.304[1], and 2.328(1) A for compounds 1-4, respectively. Structural and electrochemical data clearly indicate that the axial ligands impart a significant influence on the electronic structures of diruthenium species.

Journal Article↗