Biomedical subjects

Toyohiko Yatagai

Publications and source records attributed to Toyohiko Yatagai.

9 recordsLinked to original sources

PubMed2006

Three-dimensional imaging of macular holes with high-speed optical coherence tomography.

OBJECTIVE: To demonstrate the advantages of 3-dimensional imaging of macular hole pathology using new-generation high-speed optical coherence tomography (OCT). DESIGN: Prospective observational case series. PARTICIPANTS: Twenty-one eyes from 20 consecutive patients diagnosed with a macular hole. METHODS: A prototype high-speed OCT system was built based on a Fourier-domain OCT (FD OCT) technology for patient examination. The system has achieved sensitivity of approximately 98 decibels, axial resolution of approximately 4.3 mum in tissue, and an acquisition rate of approximately 18,700 axial scans per second. Three-dimensional imaging of macular hole pathology was performed based on a raster scan protocol consisting of 256x256 axial scans. All patients were imaged with 3-dimensional OCT, Stratus OCT, and OCT Ophthalmoscope C7. MAIN OUTCOME MEASURES: Images of macular hole pathologies obtained by 3-dimensional OCT and standard OCT instruments. RESULTS: The 3-dimensional OCT imaging successfully generated realistic 3-dimensional images of the vitreofoveal interface and intraretinal microstructures associated with a macular hole. The 3-dimensional overview of the vitreofoveal interface was helpful in gaining an immediate understanding of the dynamic interactions of the vitreous and fovea. Observations of consecutive en face images in combination with conventional longitudinal images and of cross-sectional images in combination with sectioned volume images enabled identification of intraretinal microstructures and their 3-dimensional extension associated with a macular hole, such as subfoveal structural changes after vitreous traction, connection of the flap to intraretinal structures, the external limiting membrane (ELM) and its disruption, and elevated photoreceptor inner and outer segments delineated by the ELM. The appearance of inner-wall images of a macular hole produced by photoreceptor inner and outer segment backreflection varied throughout macular hole stages. CONCLUSIONS: Three-dimensional imaging of macular holes with high-speed OCT based on FD OCT technology offers 3-dimensional overviews that facilitate understanding of the abnormalities in the vitreofoveal interface. It also provides consecutive orthogonal images that allow much more precise and minute observation of 3-dimensionally extending intraretinal structural changes associated with a macular hole than conventional OCT imaging, especially in the photoreceptor inner and outer segments.

Aged↗

PubMed2006

Colour characterization of a Morpho butterfly wing-scale using a high accuracy nonstandard finite-difference time-domain method.

In certain species of moths and butterflies iridescent colours arise from subwavelength diffractive structures. The optical properties of such a structure depend strongly on wavelength, incidence angle and state of polarization of illuminating radiation and on the viewing angle. Such structures can be analyzed only by solving Maxwell's equations, but since analytical solutions exist for only a few simple, highly symmetric structures numerical methods must be employed. We investigated the optical properties of butterfly wings in two dimensions by simulating a scale structure using a high accuracy version of nonstandard finite-difference time-domain algorithm. The simulated structure is a computer-generated model of a certain quasi-periodic arrangement of tree-like structures observed in the transmission electron micrograph (TEM) image of a transverse cross-section of a single scale from Morpho butterfly wings. We assumed that the structure is made of a slightly lossy dielectric material. We checked the accuracy and validity of our approach, by computing scattered field intensities due to an infinite cylinder and compared the results with analytical calculations using Mie theory. Next we deduced the wavelength dependence of a real refractive index and an absorption coefficient for the ground scales on the wings of Morpho sulkowskyi butterfly by computing the reflectivity and transmissivity spectrum of a scale at normal incidence, and comparing with experimental measurements. Finally, we calculated the tristimulus values and corresponding colour coordinates for various viewing directions from the scale's far-field reflectivity and transmissivity spectra to characterize its colour rendering abilities.

Journal Article↗

PubMed2006

Fast calculation method for spherical computer-generated holograms.

The synthesis of spherical computer-generated holograms is investigated. To deal with the staggering calculation times required to synthesize the hologram, a fast calculation method for approximating the hologram distribution is proposed. In this method, the diffraction integral is approximated as a convolution integral, allowing computation using the fast-Fourier-transform algorithm. The principles of the fast calculation method, the error in the approximation, and results from simulations are presented.

Journal Article↗

PubMed2006

Simultaneous B-M-mode scanning method for real-time full-range Fourier domain optical coherence tomography.

High-speed complex full-range Fourier domain optical coherence tomography (FD-OCT) is demonstrated. In this FD-OCT, the phase modulation of a reference beam (M scan) and transversal scanning (B scan) are simultaneously performed. The Fourier transform method is applied along the direction of the B scan to reconstruct complex spectra, and the complex spectra comprise a full-range OCT image. Because of this simultaneous B-M-mode scan, the FD-OCT requires only a single A scan for each single transversal position to obtain a full-range FD-OCT image. A simple but slow version of the FD-OCT visualizes the cross section of a plastic plate. A modified fast version of this FD-OCT investigates a sweat duct in a finger pad in vivo and visualizes it with an acquisition time of 27 ms.

Computer Systems↗

PubMed2006

Polarization contrast imaging of biological tissues by polarization-sensitive Fourier-domain optical coherence tomography.

Jones matrix imaging of biological samples by a polarization-sensitive Fourier-domain optical coherence tomography has been demonstrated using a two-dimensional CCD camera to obtain two spectra corresponding to the orthogonal polarization components simultaneously. The measurement results of a quarter-wave plate are compared between the two incident polarization sets, H-V linear and R-L circular polarization. Jones matrix imaging of the bovine tendon is demonstrated. Measured Jones matrix images are converted to equivalent Müller matrix images. Local polarization properties are obtained by longitudinal differentiation of Jones matrix components. The layered structure of the bovine tendon and birefringence are revealed.

Algorithms↗

PubMed2003

Holographic three-dimensional display synthesized from three-dimensional fourier spectra of real existing objects.

A method of synthesizing computer-generated holograms of real existing objects is proposed that is based on a series of projection images of an incoherently illuminated object recorded from different perspectives. In accordance with the principles of computer tomography, the three-dimensional Fourier spectrum of the object is calculated by use of several projection images. A method of calculating a Fresnel hologram from the three-dimensional Fourier spectrum is proposed. Experimental results in the form of a computer simulation and optical reconstruction are presented.

Journal Article↗

PubMed2003

Treatment of nevus using medical tattooing.

Medical tattooing is used to color skin with a pigment loss. Currently, however, a trial-and-error scheme is employed to obtain the desired color appearance of tattooed skin because prediction of the color appearance is dependent on the experiences of medical doctors. We propose a method for predicting the color appearance of tattooed skin. Two trial dyes are first injected in the area of pigment loss, and the color appearance of a third dye to be injected can be predicted using measured spectrocolorimeter data and mathematical formula. The spectrocolorimeter measures the color appearances of the skin before and after tattooing using the first two dyes, and the mathematical formula calculates the color appearance of the tattooed skin using any third dye. In the derivation of the mathematical formula, light propagation in the skin has been modeled using the modified Lambert-Beer law considering the strong scattering of light by biological tissues. The proposed method was successfully validated by a preliminary tattooing of the skin to an area with pigment loss. Predicting the color appearance of tattooed skin significantly reduces the number of trial-and-error attempts required in the current methods. Medical tattooing using this method can also be applied to treat various skin color abnormalities such as leukoderma, intradermal nevi, and reconstructed nipples.

Colorimetry↗

PubMed2002

Adaptive binary joint transform correlator for image recognition.

Our research has shown that the autocorrelation peaks of a binary joint transform correlator are affected by input scenes' backgrounds. An adaptive method is proposed to overcome this problem. The image of interest is first extracted from the background based on the position of the highest correlation peak of the input and reference images. The extracted image is then correlated with the reference to obtain the final correlation peak. Numerical simulations showed that the final autocorrelation peak is the maximum constant for a specified reference image.

Journal Article↗

PubMedDate not supplied

Three-dimensional line-field Fourier domain optical coherence tomography for in vivo dermatological investigation.

We demonstrate 3-D optical coherence tomography using only 1-D mechanical scanning. This system uses the principle of Fourier domain optical coherence tomography for depth resolution, 1-D imaging for lateral vertical resolution, and mechanical scanning by a galvanometer for lateral horizontal resolution. An in vivo human fingerpad is investigated in three dimensions with an image size of 480 points (vertical) x 300 points (horizontal) x 1024 points (depth), which corresponds to 2.1 x 1.4 x 1.3 mm. The acquisition time for a single cross section is 1 ms and that for a single volume is 10 s. The system sensitivity is 75.6 dB at a probe beam power of 1.1 mW.

Dermoscopy↗