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Biomedical subjects

U Abbuehl

Publications and source records attributed to U Abbuehl.

2 recordsLinked to original sources

Open reduction and bone plate stabilization, compared with closed reduction and external fixation, for treatment of comminuted tibial fractures: 47 cases (1980-1995) in dogs.

OBJECTIVE: To compare open reduction and bone plate fixation with closed reduction and external skeletal fixation as treatment for severely comminuted fractures of the tibia. Limb alignment, fracture reduction, operating time, hospitalization time, postoperative care, time to unrestricted activity, bone healing, complications, and number of surgical procedures were considered. DESIGN: Retrospective case series. ANIMALS: 47 dogs with severely comminuted fractures of the tibia treated with open reduction and bone plate application (22 dogs) or closed reduction and external fixation (25 dogs). PROCEDURE: Medical records of all dogs included in this study were reviewed. Postoperative and follow-up radiographs were evaluated by 2 independent observers. RESULTS: Differences were not found in hospitalization time, time to unrestricted activity, or time to earliest radiographic evidence of bone healing between dogs with fractures treated with a bone plate and dogs with fractures treated with an external fixator. Fractures treated with an external fixator had more caudal malalignment, and fractures treated with a bone plate had more valgus malalignment. Malalignments were determined not to be related to clinical problems. Dogs with fractures treated with an external fixator had shorter surgery times and more recheck examinations. Dogs with fractures treated with a bone plate had more complications. CLINICAL IMPLICATIONS: Open reduction with bone plate fixation and closed reduction with external fixation were both effective for treatment of comminuted tibial fractures. External fixation was associated with shorter surgery time, but dogs required more extensive postoperative care. Bone plate fixation was associated with more complications.

Animals↗

Nuclear triiodothyronine binding sites in rat adipocytes.

Most effects of thyroid hormones appear to be mediated by the binding of triiodothyronine (T3) to nuclear triiodothyronine binding sites (NT3BS). Although thyroid hormones influence adipocyte metabolism, NT3BS have not been described in mature adipocytes yet. This report describes T3 nuclear binding in isolated nuclei from rat epididymal fat pad adipocytes. Nuclei were isolated by exposing collagenase-dispersed adipocytes to STM (sucrose, 0.25 mol/L; TRIS, 20 mmol/L; MgCl2, 1.1 mmol/L, pH 7.85) containing 0.5% (vol/vol) Triton X-100. Incubation of nuclei suspended in STM/EDTA (2 mmol/L)/DTT (5 mmol/L) with 125I-T3 and varying concentrations of unlabeled T3 at 37 degrees C for one hour revealed the presence of high-affinity, low-capacity NT3BS. Their MBC was 0.39 +/- 0.04 (SD) ng of T3 per milligram of DNA and their Kd was 1.4 +/- 0.5 (SD) X 10(-10) mol/L T3. Specific binding reached a plateau between 30 minutes and two hours of incubation. The addition of 5 X 10(-7) mol/L T3 to nuclei incubated for one hour with 2 X 10(-11) mol/L T3 completely displaced the specifically bound 125I-T3 within 30 minutes. Thyroxine (T4) and 3, 3', 5'-triiodothyronine (rT3) could displace 125I-T3 from the NT3BS but were less than 10% and 1% as effective, respectively, as T3. Rat epididymal fat pad adipocytes contain NT3BS, the binding characteristics of which are similar to those of rat hepatic NT3BS.

Adipose Tissue↗