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U Bosse

Publications and source records attributed to U Bosse.

15 recordsLinked to original sources
PubMed1995

Double-differential PCR for gene dosage estimation of erbB oncogenes in benign and cancer tissues and comparison to cellular DNA content.

Competitive and differential quantitative PCR methods circumvent the limiting factors of PCR which cause poor reproducibility. We describe the development and performance evaluation of another quantitative PCR method, double-differential PCR (ddPCR). The ddPCR method comprises the co-amplification of the single-copy gene HBB, the erbB-1, erbB-2 and erbB-3 oncogenes and the second single-copy reference gene SOD2 under equal reaction conditions. The ratio of band intensities of the PCR products in silver-stained polyacrylamide gels expresses the average gene copy number (AGCN) per cell of the erbB oncogenes. The coefficient of variability (CV) was less than 25% for an AGCN of 1. The PCR data were in correlation to the results from dot blotting. DNA image analysis did not reveal any correlation between DNA content and gene dosage deviation of the erbB oncogenes. The method was applied to normal breast tissue, benign breast diseases, breast cancer tissue and lymph node metastases. We suggest this method as being reproducible, low cost and rapid, and therefore suitable for clinical studies on erbB oncogene dosage estimation.

Base Sequence↗
PubMed1995

Prognostic relevance of aberrations in the erbB oncogenes from breast, ovarian, oral and lung cancers: double-differential polymerase chain reaction (ddPCR) for clinical diagnosis.

We have determined the average gene copy numbers (AGCN) of the erbB-1 gene, encoding the epidermal growth factor receptor (EGF-R), the erbB-2 and the erbB-3 genes in breast, ovarian, oral, and lung cancer tissue by using double-differential PCR (ddPCR). The ddPCR method comprises the co-amplification of the single-copy gene HBB, the erbB-1, erbB-2 and erbB-3 oncogenes and the second single-copy reference gene SOD2 under equal reaction conditions. In a retrospective study the AGCN of the erbB genes and the time up to the appearance of metastases were subjected to life-table analysis in 128 women with primary breast cancer. Patients whose breast cancer tissue showed an AGCN for erbB-1 of less than 0.4 and greater then 1.6, as expected from the literature, for erbB-2 of greater than 2.0 and for erbB-3 of less than 1.75 had decreased disease-free survival (DFS). The quotient of erbB-1 and erbB-2 AGCN was the most significant in multivariate Cox analysis followed by nodal status and progesterone receptor status. In extensive studies a similar association between erbB AGCN and metastasis was seen in ovarian cancer and oral cancer, though erbB oncogene aberrations in those entities were not as frequent as in breast cancer. The AGCN of erbB oncogenes may not be of prognostic value in untreated lung cancer patients.

Base Sequence↗
PubMed1995

[Prolactin producing hypophyseal carcinoma. Case report of an extremely rare metastatic tumor].

A 59-year-old male patient was transnasally operated on because of a pituitary adenoma with hypopituitarism. A second operation and X-ray therapy followed a half year later due to recurrent tumor. Both neoplasmas were classified as sparsely granulated prolactin cell adenomas. Immunohistochemical studies revealed strong immunoreactivity for prolactin and FSH in the tumor cells of both the pituitary adenoma and the recurrent tumor. Two years later the prolactin plasma levels were extremely elevated. A tumor in the liver was identified. Biopsy revealed a solid endocrine tumor containing prolactin by immunohistology. Due to structural and immunohistological similarities this tumor could be identified as a metastasis of the pituitary tumor. After 5 months of therapy the patient died from thrombembolism. Post-mortem studies confirmed the diagnosis of a metastasizing prolactin-secreting pituitary carcinoma. Only six similar cases have been reported in the literature. Our case report confirms the experience with 35 definite pituitary carcinomas reparted in the current literature: malignant pituitary tumors develop after pituitary surgery and can be identified not from the pituitary tumor, but only from its metastases.

Combined Modality Therapy↗
PubMed1993

[Pathogenesis and size development of infected saccular aneurysms--an animal experiment study].

Both femoral arteries of 56 Wistar rats were intersected and re-anastomosed by microsurgical technique. 24 anastomoses were performed under aseptic conditions and 88 were contaminated with a standard suspension of staphylococcus aureus. All the contaminated anastomoses which had remained patent developed true saccular aneurysms that reached their maximum volume during the acute period of infection between the 10th and 50th postoperative day. None of the anastomoses performed under aseptic conditions developed an aneurysm. This experiment represents an animal model for the reproduction of true saccular aneurysms of the arterial wall. It may help to investigate the etiology of arterial aneurysm formation.

Anastomosis, Surgical↗
PubMed1990

[Multifocal bronchial cancer].

Since the entire surface of the bronchial mucosa is exposed to carcinogenic stimuli, the possibility of developing multiple primary lung carcinomas must be discussed. Multiple primary lung carcinomas do not necessarily occur simultaneously (synchronous), they can also develop successively (metachronous). They can be localized ipsilateral as well as contralateral, and histologically they have an identical or different character. In our study of 612 patients with bronchogenic carcinoma we found 18 patients (2.9%) to have a synchronous multiple primary carcinoma. The growing clinical relevance of the diagnosis of the multiple primary bronchogenic carcinoma--which is certainly diagnosed to rarely--and the distinction from metastases will be discussed.

Adult↗
PubMed1990

[Social indication for hospitalization. Annual analysis of the pediatric department of the Magdeburg district hospital].

The patients' data of 156 children hospitalized on account of social indication were analysed at the Clinic for Pediatrics of Magdeburg District Hospital. Reasons for socially indicated hospitalization were: proved or imminent neglecting of children (49%), insufficient nursing of sick children (31%), suspected ill-treatment (8%), serious family troubles temporarily preventing nursing and safety of children (12%). Neglected hygienic condition of the body, improper nutrition of malnutrition, physical and mental lagging behind might be evidence for the social sufferings of children. Mothers of children obtaining in-patient treatment as a result of social indication predominantly lack lasting partnerships. They are Less qualified concerning vocational training or gave birth to their first child at a comparatively early age. There is an urgent necessity of closely cooperating between the Clinic for Pediatrics, the Maternity Advisory Centre, the Youth Well-Fare Service and the Department of Youth-Well-Fare in order to detect the children concerned in time to be able to find the best possible solution for the future life of the children without delay.

Child Abuse↗
PubMed1990

[The tumorlet carcinoid in bronchiectasis-changed lungs. An example of a multifocal, endocrine tumor].

Tumorlets of the lungs are multifocal hyperplasias of endocrine cells which may develop via nodular hyperplasia into peripheral, occasionally also metastatic, carcinoids. They are observed with a particular frequency in bronchiectasis. Resected material obtained from patients with bronchiectasis (4 women, 3 men, mean age 43.5 years, range 19-66 years) were submitted to conventional histological studies employing HE, PAS and van Gieson staining, and also to an immunohistological analysis. Conventional staining sufficed to identify endocrine cell proliferations in the scarred lung tissue in 3 preparations. An assignment to bronchioles and alveoli with conventional histology was, in view of the extensive scarring of the lung parenchyma, very difficult, but was accomplished with the aid of immunohistological investigation. As a multifocal tumour, the tumourlet suggests itself as a model, employing immunohistochemistry, for studying the formal genesis of neuroendocrine tumours of the lung, since it shows the earliest intra-epithelial neoplastic transformations through in situ tumour to carcinoid.

Adult↗
PubMed1988

[The acardius amorphus].

We report on an acardius in a 47-year old tenthgravida. The postnatally detected acardius did not cause any obstetrical problems. The following theory seems to be the most suitable explanation for the development of an acardius: in a twin (multiple) pregnancy with blood vessel anastomoses between both twins, a twin with a haemodynamic advantage supplies the other one with oxygen-deprived blood; the latter thus becomes an acardius.

Abnormalities, Multiple↗
PubMed1987

[Immunohistochemical diagnosis of occult breast cancer].

The techniques associated with immunohistochemistry are of major interest in modern tumour diagnosis. Their simple application provides a wider scope for diagnosis by increasing the possibilities of characterising tumour cells. In particular, the combination of various tumour markers has led to a better understanding of tumour histogenesis. If the primary tumour is unknown and only metastases are available for diagnosis, further important information can be gained allowing a correlation with the origin of the metastases. In three own cases an occult carcinoma of the female breast could be diagnosed pre-operatively via immunohistochemistry techniques. Since no marker is available that reacts specifically with cells of metastasizing mammary cancer, lymph node metastases have been examined with several different markers. For that purpose, the epithelial membrane antigen (EMA), Thomsen-Friedenreich-antigen (T-Ag) and alpha-lactalbumin (ALA) have been used to point out several pathways of cell metabolism. It could be demonstrated that the prospective use of the three markers is of the highest value to make diagnosis easier and safer than by interpreting the morphological appearance. The diagnostic value of tumour markers is an important step forward, especially in the interpretation of tumour cell embolism, macrometastases and micrometastases in regional lymph nodes.

Antigens, Neoplasm↗
PubMed1975

[Intramural gas collection in the small bowel (author's transl)].

Intramural gas collection in a bowel segment is an important roentgenological sign, consistent with necrosis of the bowel wall. This is demonstrated by an example of jejunal volvulus and an inflammatory conglomeration of intestine. The diagnostic interpretation must be completed by the clinical picture and other signs of intestinal ischemia.

Abdomen, Acute↗