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Biomedical subjects

U Falkenhagen

Publications and source records attributed to U Falkenhagen.

At least 37 records · Page 2Linked to original sources
PubMed1986

[Phagocytosis in patients with chronic pyelonephritis].

In 30 patients with a non-obstructive chronic pyelonephritis and two to three clinically manifest urinary tract infections per year as well as in 50 healthy test persons we determined the rate of phagocytosis and the index of phagocytosis of neutrophil granulocytes in absence of serum with Candida albicans and latex particles in double disposition. The mean values of the phagocytosis rate und phagocytosis index of the patients were significantly decreased both for Candida albicans with 40.80% and 1.29 and for latex particles with 67.80% and 2.33 compared with healthy test persons with 54.24% and 1.69 and 86.54% and 3.51, respectively, disturbances of the cell metabolism of the neutrophil granulocytes with an impairment of the function of the cell membrane were discussed as cause of the reduced effect of phagocytosis.

Adult↗
PubMed1985

[Occurrence of Escherichia coli K1 and K5 strains in patients with urinary tract infections].

1499 E. coli isolates from patients with urinary tract infections were investigated for the presence of K1 and K5 strains using specific phages. K1 antigen was detected in 10,5% and K5 in 6,1% of the strains examined. The strains were isolated in two laboratories with different hospitals and outpatient departments. With respect to the isolation area and isolation time the frequency of K1 and K5 strains was found to be different.

Bacteriophage Typing↗
PubMed1984

[Serum resistance of Escherichia coli in chronic pyelonephritis. 1. Serum resistance in the human serum pool].

123 patients of the kidney department of the Clinic for Inner Medicine of Rostock University suffering from chronic pyelonephritis were taken into microbiological observation for between one and four years. 170 E. coli strains were bred from 59 patients with significant bacteriuria in the course of the disease and their serum resistence was determined with pooled human serum using Taylor's method. 78.24% of the strains examined were serum-sensitive, 11.18% intermediate and 10.59% serum-resistent. All strains were O-, K- and H-typed. 57.06% were successfully O-typed and were distributed over 40 O-serogroups. 24.12% were not typable and 18.82% were rough colonies. 86.50% of the resistent and intermediate strains strains were O-typable, 13.50% could not be typed. The significance of E. coli antigens (O, K, H) and serum resistence for the maintenance of a chronic infection is discussed.

Antigens, Bacterial↗
PubMed1984

[Serum resistance of Escherichia coli and serum bactericidal activity in chronic pyelonephritis].

123 patients with chronic pyelonephritis of the renal dispensary of the clinic of internal medicine of the Department of Medicine Wilhelm Pieck University Rostock were controlled microbiologically for one to four years. Of 59 patients of this group with significant bacteriuria we cultivated 170 strains of E. coli, which were standardised O, K, and H. Of all causative organisms the serum resistance was determined in a human serum pool. All sera of patients were examined for their bactericidal activity to the homologous E. coli urine strain as well as to a serum-sensitive E. coli reference strain (E. coli O 22:K-:H+). In 11.8% we found in the sera investigated at the time of the excretion of causative organisms disturbances of the serum bactericidal action, which rendered possible the survival of the sensitive in the human serum pool causative organism. In 8.82% of the sera of patients at the time of bacteriuria there was a decreased bactericidal action against the E. coli reference strain, only two of these sera did also not kill the urine strain of the patients. In individual cases sera of patients were examined for bactericidal activity with the homologous germ after absorption. By this absorption the antibactericidal activity of the sera could be removed. The proof of a disturbance of a bactericidal action may be an important reference for the course of the chronic pyelonephritis.

Blood Bactericidal Activity↗
PubMed1983

[Immunoadsorption using Staphylococcus aureus, COWAN I strain].

Immunoadsorbents bind immunoglobulins, immunocomplexes or immunocytes. Their clinical use could become a new therapeutic principle for diseases in the pathogenesis of which antibodies or immunocomplexes play an important part. Immunoadsorbents are also being tested in the hope that they can at least partially replace the plasmapheresis-plasma exchange therapy at present practised. A report is given on in-vitro studies using protein-A-bearing staphylococci, strain COWAN I. The bacteria bind IgG selectively, IgM to a small extent, IgA, IgD and IgE hardly. The majority of the other plasma proteins tested (e.g. albumin, transferrin, ceruloplasmin, alpha-2-macroglobulin, beta-lipoprotein, alpha-2-glycoprotein, alpha-1-antitrypsin) are bound only non-specifically and in small quantities. The staphylococci can react with acid solutions (glycin-HCl buffer, acetic acid) in several ways. The activation of the complement system by IgG binding to protein A is one of the problems to be solved before immunoadsorbents an be used clinically.

Antigen-Antibody Complex↗
PubMed1983

[Detection of antibody-coated bacteria in the urine of adults with chronic pyelonephritis].

Urine samples of 59 patients suffering from chronic pyelonephritis were tested repeatedly for antibody-coated bacteria (ACB). We demonstrated that the number of positive results increased with the frequency of the tests performed. It was observed, however, that antibody-coated bacteria were not found consistently in every sample during a longer period of observation.

Adult↗
PubMed1982

[The effect of plasmids of various incompatibility groups on the serum sensitivity of Escherichia coli K 12].

Using the method of Henkel and Taylor (11, 18) 60 Escherichia coli K 12 J 53 strains carrying R-plasmids of 15 incompatibility groups (FI, FIme, FII, FIV, FV, FVI, I alpha, I gamma, I omega, I5, B, C, K, M, S) have been tested with respect to their serum sensitivity in pooled human serum. We found for 41 plasmid carrying strains a grade of resistance to serum bactericidal activity of 0, for 19 strains a grade of 1, 2 or 3 according to the method of Henkel. Applying the method the method of Taylor the results of the bactericidal tests of the same 60 strains could all graded in category 1. The results obtained show that there was not any change of sensitivity to serum bactericidal activity after the uptake of a plasmid. 29 strains carrying different plasmids of the same 15 incompatibility groups were selected for studies in a low concentration system of serum. Survivors' curves in 1, 2, 3, 5, 10 and 20% of serum after different times of incubation (10, 20, 30, 60, 90, 120 and 180 min) were established. In relation to the control strain E. coli K 12 J 53 R- a difference in the percentage of survivors of 18 strains carrying plasmids of 10 incompatibility groups (FI, FIme, FIV, FVI, I alpha, I omega, I5, C, K, and M) could be demonstrated. However, this could only be observed in concentrations of serum from 1 to 5% and most clearly after an incubation time of 10 min. The results were graded into 3 categories in dependence of the difference of the percentage of survivors to the control strain. The plasmid pIE 307 and pIE 403 (Inc FI and C) showed the strongest activity in increasing the serum resistance. We found the method used to be suitable for the estimation of small alterations of serum sensitivity. The results obtained in E. coli K 12 made us control their influence in other E. coli strains carrying a complete O-antigen.

Blood Bactericidal Activity↗
PubMed1980

[Immunostaging of patients with chronic pancreatitis. I. A study of humoral and cell-mediated immunity (author's transl)].

Immunostaging was performed in patients with chronic pancreatitis of different etiologic groups. Immune responifveness was assessed by immunoglobulin serum levels, non specific autoantibody formation, C3 levels, antistreptolysin reaction, antibody titer against Toxoplasma gondii, E. coli, Herpes simplex-, Mumps- and Cytomegalovirus and screening for soluble immune-complexes (PEG precipitation assay), number of T- and B-lymphocytes, lymphocyte responsiveness to PHA, PPD, tetatoxoid and mumps-antigen in a whole blood assay, skin testing with candidin, trichophytin, tuberculin and streptokinase and HLA-typing. In some cases we found a depressed cell-mediated immune responsiveness. Patients were divided into two groups based on this main finding. Cellular hyporesponsiveness was correlated to the severity of the disease and a high incidence of HLA-B5 but not to any etiologic factor of the disease.

Adult↗
PubMed1979

Epstein-Barr virus infections in childhood.

IgM and IgG antibodies against Epstein-Barr virus (EBV) capsid antigen and antibodies against EBV nuclear antigen and heterophil antibodies were investigated in 115 paired sera of children with acute infections and in 100 sera of healthy controls of the same age and sex. EBV-specific IgM antibodies could be recognized in 13.7% of the patients and in 7% of the controls. Antibodies against EBV nuclear antigen were not detected in the IgM-positive sera.

Adolescent↗
PubMed1979

[Serum complement and protein metabolism in chronic dialysis patients].

1. When a so-called free diet is granted there is the danger of a protein deficit, which can be proved in a significant decrease of the serum transferrin and of the complement factor E3c, in patients in the chronic haemodialysis programme. 2. Within the group undergoing dialysis a correlation analysis did not result in a statistically ascertained connection between the complement factor C3c and the total haemolytic activity and the transferrin, respectively. 3. On the basis of a diet analysis a connection between the protein supply and the serum transferrin level could be established, which was not to be proved for the complement factor C3c and the total haemolytic activity, respectively. 4. Low transferrin values in the serum seem to be followed by a deterioration of the anaemia situation of the patient undergoing haemodialysis. 5. Compared with the total haemolytic activity and the complement factor C3c the determination of the serum transferrin allows an essentially exacter information about the protein metabolism of the patient undergoing a chronic haemodialysis.

Blood Proteins↗
PubMed1979

Serum complement and protein metabolism in chronic dialysis patients.

1. Patients receiving regular hemodialysis treatment who are permitted to select their own diets are in danger of a protein deficiency manifested by a significant reduction in serum transferrin and in complement C3c. 2. Correlation analysis within the dialysis group revealed no secure connexion between the complement C3c and total hemolytic activity respectively, and transferrin levels. 3. Analysis of diets showed that protein intake and the serum transferrin level correlate. No such correlation was found for the complement C3c or total hemolytic activity. 4. Low transferrin levels in the serum appear to result in more severe anemia among dialysis patients. 5. Knowledge of the serum transferrin level permits much more exact assessment of the protein metabolism in regular hemodialysis patients than knowledge of the total hemolytic activity or the level of the complement C3c.

Blood Proteins↗
PubMed1976

[Principles of therapy with antibiotics and chemotherapeutics].

Important aspects for the today therapy and prophylaxis with antibiotics/chemotherapeutics resp. are represented (choice of the antibiotic, treatment with antibiotic or sulphonamide, bacteriostatic or bactericidal activity, combination possibilities with examples). A strong indication for the application of chemotherapeutic agents is demanded. The necessity of careful sensitivity tests before instituting chemotherapy is emphasised. Reasons for the essentially economical application of drugs today are referred to. Especially is discussed the problem of transferable drug resistance, the resistance situation during therapy, and the problem of drug application outside medicine.

Anti-Bacterial Agents↗
PubMed1976

[Fluorescence serologic studies for the detection of Trichinella spiralis].

Attempts were made to find out, if Trichinella (Tr.) spiralis could be detected by means of immunofluorescence. An applicable Trichinella antigen was prepared by applying pepsin digestion to trichinous muscular tissue of experimentally infected guinea pig. Different quantities of Trichinae were orally administered to guinea pig and rabbit, and even parenteral infection of rabbit was attempted, with the view to obtain immune sera. Oral infection was followed by the occurrence of antibody titres, between the tenth day from infection and the sixtieth day, when the experiment was disrupted. Highest antibody titres were established in the fourth through sixth weeks after infection. Titres in excess to 1:64 could not be recorded by means of indirect immunofluorescence. No antibody were detected by fluorescence serology after parenteral immunisation. The detection of Trichinella spiralis by fluorescence microscopy was somewhat problematic. When the preparations were evaluated, some secondary fluorescence was observed. It had been caused by remnants of undigested muscular fibrils, contaminants, and cell detritus not removable by the authors' method of preparation and, therefore, also recorded. That secondary fluorescence, however, was eliminated by secondary staining, using methylen blue. Hence, fluorescence should not pose any obstacle to automatic reading of the reaction. Some five or six hours were required to detect by means of indirect immunofluorescence Trichinella antigen as extracted from test animals. Yet, in practice carcass processing would go on in that time. The above results, consequently, appear to suggest that for detection of Trichinella spiralis immunofluorescence cannot even replace trichinoscopy under the condition that the time of antigen preparation was shortened.

Animals↗
PubMed1976

[Detection of H-antibodies in the diagnosis of listeriosis].

The technique of H-antibodies determination in serodiagnosis of listeriosis used at present is capable of detection not only H-antibodies but also flagellar somatic antibodies in the sera. An attempt was made to increase the specificity of H-agglutinins. An experience in the preparation of pure H-antigen is described. However, it is impossible to detect positive H-agglutination either with the aid of a mixture of flagellae and latex or with pure H-antigen. After determination of H-antigen by the classic Widal reaction 405 sera sent to Rostok Institute of Medical Microbiology and Epidemiology because of suspected listeriosis were subjected to absorption with O-somatic antigen of listeria; as a result of which in 85.9% of the sera the amount of H-agglutinins decreased by 1-3 dilutions. Whereas before the exhaustion 90 sera had the H-titre of 1:321 and higher, only 15 such sera remained after the exhaustion.

Adsorption↗