We have investigated the effect of Forskolin, a substance that stimulates c-AMP, on the motility of freshly thawed bull-sperm. Forskolin was added in amounts of 25.78 and 2.57 micrograms. In control samples we employed saline. An amount of 25.78 micrograms Forskolin increased significantly the procentage of motile spermatozoa compared with 2.57 micrograms Forskolin or saline. The mean velocity of the motile sperm is decreased slightly, but constantly, over a period of 130 minutes by Forskolin (25.78 micrograms), but not by 2.57 micrograms Forskolin or saline. With respect to progressive motile sperm and their mean velocity, there were no significant differences detectable. The usefulness of Forskolin in reproductive medicine needs further evaluation.

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PubMedDate not supplied

[The effect of relaxin on sperm motility. In vitro experiments with bull sperm].

We investigated the effect of relaxin (1990 pmol, 199 pmol, 19.9 pmol) on bull-sperm-motility by means of Laser-Doppler (Lazymot). We evaluated 4 parameters, i.e. percentage of motile spermatozoa and percentage of spermatozoa with good progression, mean velocity of the sperm sample and characteristic velocity of the sperm subpopulation with good progression. Amounts of 199 and 19.9 pmol relaxin did not provoke a significant change with respect to sperm motility compared with "relaxin-free" medium. The highest amount of relaxin (1990 pmol) inhibited sperm-motility with respect to all 4 parameters that had been investigated. The action of relaxin on sperm-motility depends on the amount of relaxin in the medium, e.g. activation at physiologic and inhibition at very high amounts of that substance.

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