PubMed Health⌕ Search

Biomedical subjects

U Gallone

Publications and source records attributed to U Gallone.

14 recordsLinked to original sources

[The presence of 3,4-benzopyrene in plants I. Methods].

Purification methods of vegetable extracts have been experienced during our researches on the possible presence of the aromatic polycyclic hydrocarbons (particularly 3,4 benzopyrene) in vegetables. The validity of different methods has been determined by IR spectrophotometric analysis using special apparatus (IR-DATA-STATION). Vegetables extracts have been purified efficaciously by column chromatography, saponification and special methods (Grimmer's method). The described method eliminates, nearly entirely, the polluting substances that could alternate the chemical analysis of the vegetable extracts.

Benzo(a)pyrene↗

[Action of the unsaponifiable components of the most common edible oils on the growth of the alga Euglena gracilis. Preliminary studies].

Within the sphere of the researches the biological effects of the most common edible oils (peanuts, sunflower, maize, soya and rectified olive) it has been studied the interaction between the development of the Euglena gracilis unicellular seaweed and the presence of the unsaponifiables examined in their cultures. As a biologically active substance it has been used 3,4-benzopyrene. Spectrophotometric analysis have evidenced that all the unsaponifiables, especially those of the soya seeds, caused a growth decrease of the seaweed culture. A similar effect is found in the cultures treated with aromatic hydrocarbon.

Animals↗

[In vitro action of unsaponifiable edible oils on calf renal cell cultures].

During the research concerning biological effects of the most common edible oils (peanuts, sunflower, maize, soya and rectified olive), the Authors have tested the insaponificable fractions on the calf kidney cellular monosurface. The insaponificable of the maize seeds oil determine a net decrease of the speed growth. It has also been noted a very slight increase of the cells development, in the cultures treated with the insaponificables of some other oils, compared with the samples of those not treated. As comparison substance it has been used 3,4 benzpyrene, because his toxic action on the cells is well known.

Animals↗

[Effect of the unsaponifiables of the most common edible oils on the development of E. coli bacteria].

The writers within the context of the research on the biological actions of the unsaponificables in the most common edible oils, have tested the effects produced by such unsaponificables on the culture of E. Coli. The unsaponificables, and those of seed oils of peanuts and sunflowers in particular, aid the doubling of the E. Coli. Similar research on the culture of animal cells (in vitro) and unicellular algae, are at present outstanding.

Arachis↗

[Biological effects of non-saponifiable sunflower seed oil. Preliminary research].

By inserting near the under-axillary gland of the rats Wistar (one month age) compressed material containing g. 0.250 of non-saponificable sunflower seeds oil, it has been noted an increase of the number of the mitosis in the under-axillary gland, in the lung and some mitosis in the liver. Such results are similar to those obtainable by administering, with similar method 0.050 g. of 3,4benzopyrene or 0.100 g. of the hydrocarbon oxidization products. The histological analysis have been effected on the organs of rats sacrificed one month after inoculation.

Animals↗

[Localization and individualization of abnormal carbonates in the valve of Mytilus galloprovincialis].

The cristallographic analysis of the valve of Mytilus galloprovincialis using the scansion microscopy method shows probable inclusions of Cd and Zn in the carbonate calcite layer and probable inclusions of Pb in carbonate aragonite layer. This confirms the supposition of a substitution of Ca ions from heavy metals investigated and the formation of the respective carbonates that precipitate together with calcium carbonate in the process of growth of the valve of the mollusc examined.

Animals↗

[Interaction between alkaloids of Claviceps purpurea and development of some bacteria and bacteriophages. Preliminary experiments].

It has been tested the bacteriolytic activity and the interaction among the development of the bacteriophage T4 and Ergot alkaloids. It has been determined a bacteriolytic action on the bacterial stub "E. Coli host of bacteriophage T4. It exist an interaction also among such alkaloids and the development of the bacteriophage T4, which appears through an increase of the quantity of lysis areas, becoming evident in solid bodies containing the lysing bacterium. Further researches with other bacterial and virus stubs will be effected and mentioned afterward.

Alkaloids↗

[Anionic detergents in Mytilus Galloprovincialis of the gulf of Naples].

The authors have investigated on the anionics detergents rate inside the Mytilus Galloprovincialis, used as animal-monitor of the environment state of a certain part of the Gulf of Naples. The data concerning 14 zones in which were drown mussels cultivated in the Gulf of Naples resumed in a specific list, shown an high concentration of above detergents.

Animals↗

[Eventual presence of 3-4 benzopyrene in the non-saponificable part of sunflower oil].

Within the limit of the research on the chemical composition of the nonsaponds of the edible oils, under execution in our Institute have been affected some cromatografic gas and spectoskopic analysis (U.V.) on a sample of sunflower seeds oil. Concentrating our research essentially on the determination of the presence of 3-4 benzopyrene in the above oil, we have treated about 8,3 gr. of nonsapond with appropriate chemical methodologies, in order to obtain specific samples for the polycyclic aromatic hydrocarbon analysis. The results of the cromatografic gas and spectoskopic of two fractions, obtained through the process of the preparation of the nonsapond sample, compared with those of a standard solution of 3-4 benzopyrene, exclude the presence of the hydrocarbon in the sunflower oil.

Benzopyrenes↗

[Free amino acids in calf embryos].

In this work have been reported the research results tending to establish the free amino acidis concentration, existing in the calf embryos. The free embryonal amino acidis composition has been determined through an analysis effected with "Amino Acid Analyzer". Such studies, which conern the researches under execution in our Institute on the chemical composition of the calf embryos furnish the biochemical determination of a biological material, used in some Nations like a commercial product.

Alanine↗

[Estrogens in calf embryos].

A particular technique has been restated for the analysis of extradiol and extrones. Availing ourselves of such a technique, we have determined the presence of the steroid hormones, extradiol and extrones, in the calf embryos. The above studies are framed within the sphere of the researches made in our Institute, having the scope of determining the biological effects and the chemical composition of the calf embryos, which is utilized like a commercial product in some other Nations.

Animals↗