U Hohmann — Page 2 | PubMed Health

PubMed1994

Amplification of DNA sequences in wheat and its relatives: the Dgas44 and R350 families of repetitive sequences.

The sequence of a Triticum tauschii genomic clone representing a family of D-genome amplified DNA sequences, designated Dgas44, is reported. The Dgas44 sequence occurs on all chromosomes of the D genome of wheat, Triticum aestivum, and in situ hybridization revealed it to be evenly dispersed on all seven chromosome pairs. An internal HindIII fragment of Dgas44, designated Dgas44-3, defines the highly amplified region that is specific to the D genome. The polymerase chain reaction was used to amplify a 236-bp fragment within Dgas44-3 from chromosomes 1D, 2D, 3D, 4D, 5D, and 7D, and identical copies of this region of the Dgas44-3 sequence were found among the isolates from each of the chromosomes. The Dgas44-3 sequence population from specific chromosomes differed on average by 0.22% from the original Dgas44 sequence. The Dgas44 sequence was found to differentiate between the D genome present in T. aestivum, T. tauschii, hexaploid T. crassum, T. cylindricum, T. ventricosum, in which the sequence was present in a highly amplified form and T. juvenale, T. syriacum, and tetraploid T. crassum where the sequence family was difficult to detect. Another class of amplified sequences previously considered to be rye "specific." R350, was isolated from tetraploid wheat and its dispersed distribution on chromosomes was similar to the Dgas44 family in T. tauschii. In contrast with the Dgas44 sequence family, genome specificity for the remnant R350 sequence family was not evident since it was present on all wheat chromosomes.

Base Sequence↗

PubMed1978

[Behavior of blood glucose in piglets up to the 10th day of life with reference to colostrum consumption and birth mass].

The following results were obtained from studies into the behaviour of blood glucose in 439 piglets: -The first experimental group included 48 piglets exposed to follow-up checks in the first ten days of age. Glucose values prior to the first intake of foremilk were higher than those measured afterwards in ten animals (20.8 per cent). The second experimental group included 391 piglets tested under production conditions, and a result identical to the one above was recorded from 48 animals (12.3 per cent). The conclusion is that rise of blood glucose does not take place in all piglets after the first intake of foremilk. -Blood glucose concentrations were found to go up discontinuously up to the tenth day of age, depending on birth weight and feed intake as well as on glycolytical and gluconeogenetical capacities. -Average glucose values between 50.23 and 67.37 mg/100 ml plasma were recorded from newborn piglets prior to first foremilk intake in four groups differentiated by weights a birth between 800 g and 2,200 g. A relationship was statistically secured between blood glucose concentration and birth weight of newborn piglets. The correlation factor appeared to depend on the weight at birth.

Animals↗