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U Holl

Publications and source records attributed to U Holl.

6 recordsLinked to original sources
PubMed1990

Synthetic peptides against foot-and-mouth disease--immunization with VP1-peptides of type O1-Kaufbeuren.

Coupled synthetic peptides, representing the sequences of amino acids 130-160, 141-160 and 145-160 of foot-and-mouth disease virus O1K protein VP1, induced virus-binding and virus-neutralizing antibody response in guinea pigs, rabbits, and pigs. We also detected antibody response in guinea pigs after immunization with uncoupled peptides and in cattle with 21 aa-peptide-Keyhole-limpet hemocyanin (-KLH). The best results were obtained from 21 aa-peptide-KLH and 31 aa-peptide with or without KLH or thyroglobulin as carrier. Our preliminary results show the induction of virus-neutralizing antibodies to be obviously influenced by length of the peptide as well as by the kind of carrier and coupling.

Animals↗
PubMed1986

[A hitherto unknown reaction pattern in vertebrate cells (RiV). 2. The protective effect of RiV particle preparations against foot-and-mouth disease in guinea pigs].

Further observations concerning the previously described RiV-particles are reported. They were isolated from a diploid cell line of bovine origin, embryonal duck fibroblasts and BHK-21 cells. A protective effect against foot-and-mouth-disease virus in guinea pigs could be observed following inoculation with the RiV-preparation of bovine origin. All 3 preparations isolated from the 3 cell lines showed immunologic cross reactions.

Animals↗
PubMed1979

[Structure of calf rotavirus. 1. Hydrodynamic and electron microscopic parameters of the virus].

Rotavirus was isolated from the faeces of calves afflicted with diarrhoea and purified. Isolation was preceded by chloroform treatment, oxide wax A-precipitation, as well as differential and density gradient centrifugation. Virus density in CsCl solution was 1.38 +/- 0.01 g/ml. Cell culture virus prepared without chloroform (Nebraska-type isolation) would be 1.37 +/- 0.01 g/ml in density, for comparison. The mean capsid diameters measured were 55 nm and 60 nm, sedimentation coefficents being between 450 and 478 S. The mean core diameter was 40 nm. Structure of capsid is discussed.

Animals↗