Does IgM-class rheumatoid factor disturb Candida-specific IgM- diagnostics?
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Antibodies, Fungal↗
Biomedical subjects
U Huschka
Publications and source records attributed to U Huschka.
Immunofluorescence studies in progressive systemic sclerosis (scleroderma) and mixed connective tissue disease.
Immunofluorescence (IF) investigations of the skin were performed in thirty patients with progressive systemic sclerosis (scleroderma) and eight patients with mixed connective tissue disease (MCTD). The results show that speckled epidermal nuclear immunoglobulin deposition occurs not only in MCTD but also in true scleroderma. Granular IgM deposition at the dermo-epidermal junction of light-exposed skin was detected in both groups of patients, but six of eight MCTD patients also showed a granular IgM band in non-exposed skin. Antinuclear antibodies (ANA) were demonstrated in the sera of 96% and 100% of patients with scleroderma and MCTD respectively. The pattern of nuclear IF staining in scleroderma included dense fine speckles, large coarse speckles, threads, nucleolar and centromere staining. In MCTD, by contrast, the ANA staining pattern consisted of threads. The significance of ANA titres and immunological specificities for the in vivo reaction of serum ANA with epidermal nuclear antigens is discussed.
Adult↗
[Immunopathologic studies in herpes gestationis].
In a 19-year-old Turkish primigravida with clinically and histologically confirmed herpes gestationis, immunohistological investigations revealed deposits of C3, C4, IgG, and IgM in the dermoepidermal junction. Furthermore, C3 and IgM were found in the upper dermal vessels, suggesting a concomitant vasculitis, which was also observed by capillary microscopy. ANA were present in the serum of the patient, a circulating factor capable of fixing complement to the basement-membrane (HG-factor) was demonstrable as well. Lymphocytotoxic antibodies, however, could not be detected. HLA-typing disclosed HLA-B8 haplotype.
Adult↗
Rapid separation of immunoglobulin M by immunoaffinity chromatography for detection of specific antibodies to rubella and Treponema pallidum.
A rapid and easy method for isolating IgM from serum specimens in order to detect specific antibodies against Treponema pallidum and rubella virus by routine serologic procedures is described. Serum IgM was isolated by immunoaffinity chromatography using anti-human IgM antibodies covalently bound to controlled-pore glass beads in a microcolumn. The final concentration of the IgM in the samples tested amounted to at least 16% (average 32%) of the original concentration (corresponding to a serum dilution of 1 : less than 8). IgG contamination did not exceed 0.38% of the original serum concentration. The capacity of the column was stable for at least 50 absorption/elution cycles. The new technique enables rapid and reliable detection of specific IgM by the rubella hemagglutination inhibition and Treponema pallidum hemagglutination tests.
Animals↗
Rapid detection of Treponema pallidum and cytomegalovirus specific IgM antibodies with the passive haemagglutination.
For the detection of Treponema pallidum-specific IgM antibodies in patients' serum samples four simple and rapid methods are compared and evaluated. They show decreasing sensitivity and specificity in the row listed: (1) Treponema pallidum haemagglutination (TPHA) after IgM separation by affinity chromatography on controlled-pore glass heads (anti-IgM-CPG), or (2) by ion exchange chromatography on DEAE-cellulose; (3) solid-phase haemagglutination (SPHA) in glass microvials or (4) on polysterene microplates. The latter tests have found to be inferior, although they can be considerably improved by the use of affinity chromatography--purified catch antibodies. The commercially available DEAE-cellulose microcolumn delivers a simple method for IgM separation, and in spite of a relatively high IgA contamination it proved to be useful also for CMV-specific IgM haemagglutination tests, as compared to ELISA results.
Antibodies, Bacterial↗
Comparison of different methods for the detection of rubella-specific IgM antibodies.
The rubella specific IgM titer in the serum specimens originating from healthy persons and from patients with clinical signs of rubella infection was determined by hemagglutination inhibition or hemagglutination reduction after IgM separation with the following methods: (a) density gradient centrifugation; (b) polyacrylamide agarose gel chromatography; (c) ion exchange chromatography with diethylaminoethyl cellulose columns; (d) solid-phase immunosorbent technique using microplates; (e) solid-phase immunosorbent technique using polyacrylamide microimmunobeads. Alternatively, we removed IgG and IgA by the use of protein A, anti-IgG, and anti-IgA, covalently coupled to controlled-pore glass (f). The titers obtained by the different methods showed qualitatively good correlations when combined with mercaptoethanol reduction. The quantitative measurement of specific IgM titers, however, revealed a lower sensitivity of column chromatography and methods of removal of IgG/IgA.
Antibodies, Viral↗
Control of protein synthesis in mammalian cells by aminoacylation of transfer ribonucleic acid.
The dependence of protein synthesis on the intracellular content of aminoacylated tRNA has been studied in mouse ascites tumor cells deprived for various amino acids. A remarkable reduction in net protein synthesis has been found only after a drastic decrease in aminoacylation of tRNA. The quantitative correlation of protein synthesis with the degree of aminoacylation suggests that a moderate amino acid starvation primarily influences the rate of elongation at the codon concerned. These results are in contrast to the findings previously reported for HeLa cells. Some crucial steps during the determination of intracellular aminoacyl-tRNA have been investigated. The reliability of the method employed has been discussed on a theoretical basis.
Amino Acids↗