The most commonly used assay to quantitate the response of peripheral T-cells upon stimulation with growth factors is determination of incorporated [3H]TdR. We compared this test to three other methods: 1. direct counting of cells with a Coulter type counter as reference assay, 2. a colorimetric assay using the tetrazolium dye 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium (MTT), which is a cheap and increasingly popular non-radioactive method and 3. incorporation of the thymidine analog 5-bromo-2'-deoxyuridine detection with a monoclonal antibody on cytospins. Primary human PHA-blasts from greater than 30 healthy individuals were stimulated with IL-2, IL-4 and IL-7 and assayed with up to four different methods. We discuss the advantages and disadvantages of the assays used and the effects of differences between cell preparations. We observed no significant variations between individuals for the dose dependence, but the relative efficiency of IL-4 compared to IL-2 and IL-7 was variable. This was probably due to the slower response observed upon stimulation with this factor.

Antibodies, Monoclonal↗

PubMedDate not supplied

[Complications in cerebral angiography. A study of 6698 cerebral angiographies].

The complications of 6,698 cerebral angiographies performed from 1979 to 1985 in 2,503 patients were analysed. In 5.24% of the patients (1.95% of the angiopathies) temporary complications could be observed. Temporary neurologic-psychiatric complications occurred in 1.96% of the patients (0.73% of the angiographies), most of them in middle-aged patients and in the presence in the presence of diffuse cerebrovascular disease. In 0.12% of the patients (0.05% of the angiographies) a permanent damage was recorded.

Age Factors↗