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U Loza

Publications and source records attributed to U Loza.

10 recordsLinked to original sources
PubMed1996

"Heterophile" antigen in sera of human recipients of renal allografts.

It was noted that many sera of patients with renal allograft produce distinct precipitation lines in gel diffusion tests with about 20% of infectious mononucleosis sera. The antibodies in infectious mononucleosis sera were of IgM isotope, but, interestingly, they could be removed by guinea pig kidney homogenate, which indicated that the reactions studied were of the Hanganutziu-Deicher rather than of the Paul-Bunnell type. This contention was strengthened by the fact that positive transplantation sera reacted also with standard serum with Hanganutziu-Deicher antibodies. Thus far, the presence of the antigen in the transplantation sera could not be related to the clinical status of the patients, however, the antigen was noted primarily in those sera that did not contain heterophile transplantation antibodies. It was proposed that the antigen detected in the transplantation sera was an altered tissue antigen released from the grafted organ. Besides, interactions between two serum samples from the same patient were noted in immunodiffusion tests. These reactions occurred very seldom and were unrelated to heterophile transplantation antigens or antibodies.

Animals↗
PubMed1975

Studies on heterophile antibodies in transplantation sera.

By means of double diffusion in gel reactions, "heterophile" antibodies were demonstrated in human renal transplantation sera. Of thirty-two recipients of renal allografts, 12 (37 per cent) had antibodies to extract of bovine erythrocyte stromata, and 5 (19 per cent) of 26 recipients produced antibodies to extract of sheep erythrocyte stromata. These antibodies became detectable 1-6 months after transplantation and persisted for several months or years. However, the strength of reactions given by individual serum samples from the same recipient varied considerably. Sera from the same recipient gave a reaction of identify, while sera from two different recipients frequently gave reactions of partial identity or nonidentity. The antibodies reacting with bovine stroma extract were distinct from those reacting with sheep stroma extract. Evidence was also presented that the heterophile antibodies in transplantation sera are different from Paul-Bunnell antibodies in infectious mononucleosis sera. Some of the HL-A typing sera were shown to contain antibodies against bovine stroma extract. The antigen recognized by these sera in the bovine stroma extract was not related to any HL-A specificity. Three of eight rabbits which received skin and renal allografts formed antibodies against bovine stroma extract. Absorption studies clearly demonstrated that these antibodies are directed against antigens present in the donor's, but not in the recipient's tissues.

Absorption↗
PubMed1975

Double diffusion in gel tests with Paul-Bunnell antibodies of infectious mononucleosis sera.

Double diffusion tests in gel were employed for studies of reactions between infectious mononucleosis sera and extracts of bovine, sheep and equine erythrocyte stromata. The extracts were obtained by ultrasonication of stromata prepared from trypsin-digested erythrocytes. The reaction with bovine stroma extract was composed, in many instances, of two lines. A single line was observed in reactions with sheep and equine stroma extracts. This line merged into a reaction of partial or complete identy with one of the lines formed with bovine stroma extract. Evidence was obtained that some infectioous mononucleosis sera may contain heterophile (Paul-Bunnell) antibodies belonging to IgG class in addition to those of IgM nature.

Animals↗