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Biomedical subjects

U N Riede

Publications and source records attributed to U N Riede.

At least 73 records · Page 4Linked to original sources
PubMed1980

Glycogenosis type I (glucose 6-phosphatase deficiency): I. Ultrastructural morphometric analysis of juvenile liver cells.

The essential biochemical characteristic of von Gierke's disease is an inborn glucose-6-phosphatase deficiency and glycogen storage in the liver and kidney. This expresses itself morphometrically as an increased volume of glycogen per unit volume of the hepatocellular cytoplasm. Since glucose-6-phosphatase activity in patients studied is practically at the zero level, and the endoplasmic reticulum loses a large part of its membrane values, we conclude that the remaining endoplasmic reticulum represents glucose-6-phosphatase free membranes. A typical structural feature of the endoplasmic reticulum in von Gieke's disease is the appearance of "double contoured vesicles" (= pockets). These vesicles comprise approximately 3,5% of the total membrane system. The mitochondria play an important role in glycolysis and glycogen synthesis. It is thus to be expected that these organelles change in terms of their morphometric parameters in the course of glycogenosis type I. An important point in this direction is numerical mitochondrion reduction in combination with an unchanged mitochondrial volume.

Child, Preschool↗
PubMed1980

Reaction of oxytalan fibers in human periodontium to mechanical stress. A combined histochemical and morphometric analysis.

A combined histochemical and morphometric analysis was performed on normal and mechanically stressed periodontal tissue obtained from upper first premolars of ten boys. Oxytalan fibers were demonstrated histochemically according to the method of Fullmer and Lillie. Quantitative and qualitative oxytalan fiber alterations were evaluated by a morphometric analysis. The morphometric results proved that the oxytalan fiber reaction to increased mechanical stress was fibroplasia, fiber lengthening, and narrowing of the fiber diameter. No differences existed between the oxytalan fiber reaction in tension and pressure zones. These findings imply that the processes of functionally induced remodeling are not the same in the soft and hard tissue.

Child↗
PubMed1980

[Reversibility of shock-induced pulmonary fibrosis (author's transl)].

A case of shock-induced respiratory failure is reported and the diagnostic criteria of this condition are reviewed. As regards the anatomicopathological aspects the following factors are of prognostic importance: 1. pre-existing pulmonary disease, 2. integrity of the alveolar basal membranes, 3. proliferation of the pulmonary morphological elements, 4. development of immature collagenous and elastic fibres in the alveolar and extra-alveolar interspaces. A diagnostically reliable estimation of these factors is possible only by means of biopsy. Although this procedure carries some risks the incidence of complications is not higher than it is in respect of biopsies of other parenchymatous organs. However, it is advisable to reserve lung biopsies for those cases where all other non-invasive methods have failed to establish the diagnosis.

Adult↗
PubMed1980

[Shock-induced respiratory insufficiency (clinical aspects, pathology ultrastructure and morphometry)].

Interstitial oedema in the alveolar septa is the first morphologically recognisable change to be observed in cases of shock. It is brought about by the altered function of the membranes of the damaged epithelium and endothelium in the alveolar wall. At the same time there is an impairment of gaseous exchange, which is rendered more difficult by the exudative process in the interstitium. Pari passu with these events there is injury to the cells of both the alveolar epithelium and the alveolar capillary endothelium. Both these processes are still reversible. The point of irreversibility appears to be reached - so far as time is concerned - at the end of the 1st week, after which the injurious effects on the cell are established, since the thin alveolar wall, necessary for the exchange of gases, becomes overgrown with bulky alveocytes (Type II) and the fibroblasts in the alveolar interstitium crowd the capillaries away from the surface of the alveolus. In most of the advanced cases of shock this process of thickening of the alveolar wall exceeds the critical value, and respiratory exchange is so impaired that satisfactory functioning of the lungs is no longer possible.

Humans↗
PubMed1979

Morphologic development of human shock lung.

In the initial phase of shock, edema spreads throughout the alveolar interstitium even before injury occurs in the alveolar epithelium and endothelium. The endothelium and the epithelium are damaged only subsequently, causing reduction in the average barrier thickness of the epithelium and the endothelium. A point of irreversibility is reached by the end of the first week. While early cell regeneration may be observed within the alveolar endothelium and epithelium, proliferation of fibroblasts and fibrosis of the alveolar wall occur in addition to edema which spreads within the interstitium. This widening of the gas exchange barrier may be considered as the anatomic substrate of respiratory insufficiency induced by shock. This enlargement continues up to the moment when thickening of the alveoli impedes satisfactory functioning of the lung, and, as consequence, threatens the life of the patient.

Cell Division↗
PubMed1979

Morphometrical analysis of an ultrahistochemical demonstration of nonspecific esterases in hepatocytes of mice after fructose overload.

Changes in ultrastructural distribution patterns of nonspecific esterases (E.C. 3.1.19) are described quantitatively by means of morphometry. Esterases were demonstrated with O-acetyl-8-hydroxyquinoline (Q-O-2) and S-acetyl-8-mercaptoquinoline in livers of normally and exclusively fructose-fed mice. Conditions are discussed, under which the quantification of the ultrastructural products of enzyme histochemical reactions may be possible. Smooth endoplasmic reticulum and rough endoplasmic reticulum exhibit no alteration in enzyme distribution with both substrates since the enzyme-occupied proportion of each compartment remains the same despite an overall decrease of both compartments. Likewise an increase of O-acetyl-8-hydroxyquinoline-esterase at fat droplets corresponds to the increase in total surface of the fat. S-acetyl-8-mercaptoquinoline-positive fat surface however reveals an increase far beyond that of the total fat surface. The results support the hypothesis that a variety of esterases with different substrate spectra are present at the subcellular level in different cell compartments.

Animals↗
PubMed1978

[Ultrastructural morphometric changes in spiral ganglion cells of the rat after broad band noise (author's transl)].

The comparison of ultrastructural and ultrastructural-morphometric findings in spiral ganglion cells subsequent to broad noise with 105 dB SPL over a period of 40 min demonstrates that there are morphometric changes of the cytoarchitecture of granular ganglion cells even without evident ultrastructural changes. Morphometric analyses shows a significant extension of the volume proportion of mitochondria per unit volume cytoplasm. The volume and the surface of the rough endoplasmic reticulum, the volume of the free ribosomes and the average single volume of the nucleoli increase per granular ganglion cell. The physiologic significance of these changes is discussed. The results show that changes of the cytoarchitecture of granular spiral ganglion cells are earlier detected by morphometry than by ultrastructural description.

Acoustic Stimulation↗
PubMed1978

The weight of human lungs as a diagnostic criterium (distinction of normal lungs from shock lungs by histologic, morphometric and biochemical investigations).

Lung weight, extravascular water content, hemoglobin content as an index of blood congestion, and DNA content as an index of cellular content, were determined for 57 patients dying from multiple, extrathoracic trauma. This report, which confirms previous publications, concerns itself in particular with the problems of determination of the "normal" lung weight. Studies of the influence of survival time on the lung showed that lung weight may significantly increase to twice its value after very short times up to one hour after trauma as a result of blood congestion, and following longer survival may be elevated several times as a result of developing interstitial edema. Early appearing interstitial edema is morphometrically detectable already 10 minutes after the beginning of shock. Normal weights were obtained from 10 cases after immediate death following severe cranial trauma or decapitation by being run over. Our results show that normal lungs have an average weight of 234 g (s +/- 39). Literature values for "normal" lung weight are approximately twice as high.

DNA↗
PubMed1978

The pulmonary air-blood barrier of human shock lungs (a clinical, ultrastructural and morphometric study).

Interstitial edema in the alveolar septa is the first morphologically recognisable change to be observed in cases of shock. It is brought about by the altered function of the membranes of the damaged epithelium and endothelium in the alveolar wall. At the same time there is an impairment of gaseous exchange, which is rendered more difficult by the exudative process in the interstitium. Pari passu with these events there is injury to the cells of both the alveolar epithelium and the alveolar capillary endothelium. Both these processes are still reversible. The point of irreversibility appears to be reached--so far as time is concerned--at the end of the first week, after which the injurious effects on the cell are established, since the thin alveolar wall necessary for the exchange of gases becomes overgrown with bulky alveocytes (Tpye II), and the fibroblasts in thealveolar interstitium push the capillaries away from the surface of the alveolus. In most of the advanced cases of shock this process of thickening of the alveolar wall exceeds the critical value, and respiratory exchange is so impaired that satisfactory functioning of the lungs is no longer possible.

Adult↗
PubMed1978

Is shock-induced lung fibrosis reversible? A report on recovery from "shock-lung".

As the consequence of a road accident a 19-year-old man developed respiratory insufficiency. Radiology and gas analysis revealed signs of "shock-lung". Morphometric analysis of a punch biopsy from the lung confirmed the diagnosis. Gas exchange grew worse in spite of conventional treatment. At the end of the third week after the trauma the maintenance of a highly negative fluid balance and the administration of high doses of corticoids were given a trial. The patient recovered. Although no functional pulmonary deficiencies remained, years later the patient still shows radiological evidence of extensive scarring. The lung parenchyma is intact, as judged by morphometric examination. This case shows that patients with "shock lung" can recover, but scars in the extrapulmonary space can remain, even though the lung parenchyma returns to normal.

Accidents, Traffic↗
PubMed1978

Standardized fixation of human lung for radiology and morphometry; Description of a "two chamber"-system with formaldehyde vapor inflation.

In order to obtain optimal preservation of lungs for histology, morphometry and radiology a two-chamber system of lung fixation with hot formaldehyde vapor is introduced. Compared with other procedures this system offers small dead space and ensures quick and thorough fixation by imitating "inspiration" and "expiration". Volume- and pressure-regulated "breathing" enables fixation in a stage of the respiration cycle, which can be chosen. This procedure can be used routinely in the autopsy room. The only serious drawback seems to be air pollution, if the chambers are not absolutely insulated.

Air Pollution↗