T-colonies in agar culture: clonal proliferation of T-progenitors or PHA-transformation of mature T-lymphocytes?
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Biomedical subjects
Publications and source records attributed to U O Topuz.
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In seven patients with polycythemia vera, the agar colony growth of bone marrow total nucleated cell suspensions and of the cell fractions obtained with an albumin discontinuous density gradient were studied. In one patient, the density distribution of colony-forming units in culture (CFUc) before and after alkylating treatment was evaluated and cell differentials on smears obtained from each density fraction were determined. A high percentage of low density CFUc compared with the total CFUc population and with that in the normal control subjects in the same density fractions was observed.
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