Biomedical subjects

U Siesenop

Publications and source records attributed to U Siesenop.

6 recordsLinked to original sources

PubMed1999

Characterization of Malassezia species by means of phenotypic characteristics and detection of electrophoretic karyotypes by pulsed-field gel electrophoresis (PFGE).

A total of 220 isolates of the genus Malassezia obtained from affected skin of animals and humans were included in this study. The isolates were examined for their belonging to the known species Malassezia pachydermatis, Malassezia sympodialis, Malassezia furfur, Malassezia obtusa, Malassezia restricta, Malassezia globosa and Malassezia slooffiae by means of phenotypic and biochemical characteristics as well as their ability to assimilate polyethylen(20)-sorbitan-esters (Tween 20, 40, 60, 80). The electrophoretic karyotypes of all isolates were studied by pulsed-field gel electrophoresis. Of the 220 Malassezia isolates examined, 217 were identified as M. pachydermatis and three were classified as M. sympodialis. Moreover, the proof of limited lipid-dependence of M. pachydermatis indicated that the commonly used criterion 'lipid-independence' is not sufficient to differentiate this species from the other known Malassezia species.

Animals↗

PubMed1998

[Phenotypic characterization of equine Dermatophilus congolensis field isolates].

In 1993 and 1994 a highly increased occurrence of equine dermatophilosis was observed, and a study was initiated to determine phenotypic heterogeneity among 120 clinical isolates using biochemistry, antibiotic resistance profiles, membrane protein profiles and Western blotting. The biochemical examinations contained 1% equine serum in medium. Moreover, the API ZYM-test from bioMérieux was used. The biochemical reactions were suited to identify Dermatophilus congolensis but did not allow a differentiation among the various isolates. Antibiotic resistance in one or more isolates was observed against polymyxin B, enrofloxacin, oxacillin, neomycin and trimethoprim-sulfamethoxazol. All isolates were sensitive penicillin G, ampicillin, streptomycin, gentamicin, lincomycin, erythromycin, tetracycline, oxytetracycline, bacitracin and ceftiofur. The evaluation of silver-stained and immuno-stained membrane protein profiles showed minor differences among several isolates. In total, all isolates appeared to be closely related and the minor differences observed did not correlate with the geographic origin of the respective isolates.

Actinomycetales↗

PubMed1997

[The pig as dead-end host of Trichophyton verrucosum? A case report].

A dermatomycosis of swine caused by Trichophyton verrucosum is reported. Trichophyton verrucosum could be identified in skin scrapings of pigs and contacting calves by fluorescence microscopy and culture. The mode of transmission and the rare isolation of Trichophyton verrucosum from pigs are discussed.

Animals↗

PubMed1997

[Case report: decreased laying performance as a contributing problem].

In 2 laying hen flocks (housed in aviary systems) depressed performance, increased mortality and cannibalism were observed. Specific infectious diseases were excluded. The hygienic quality of the diet, however, based on self produced feed ingredients, was impaired. High concentrations of bacteria, moulds, and yeasts were found in the complete diet and also in the ingredients. These problems were eliminated by providing the birds with good quality feed ingredients. In a diagnostic feeding trial with a change in housing conditions hens showed a good performance. The poor feed quality, as demonstrated in this report, is regarded to be one of several factors, which have contributed to the problems observed in these flocks. Other negative environmental factors such as housing have to be eliminated.

Animal Feed↗

PubMedDate not supplied

Comparative studies on keratinase production of Trichophyton mentagrophytes strains of animal origin.

Over a period of 28 days, 10 Trichophyton mentagrophytes strains were examined for their ability to secrete keratinolytic enzymes. Production of enzymes was stimulated by various keratins used as substrates. Duration and intensity of keratinase secretion were strongly influenced by the keratinous substrate. Duration and intensity of the enzyme production also differed among the 10 dermatophyte strains. Five different enzymes were isolated with molecular weights ranging from 28 kDa to 65 kDa. The different enzymes might be produced by different varieties of the species Trichophyton mentagrophytes.

Animals↗

PubMedDate not supplied

Studies on growth, spore-forming ability and virulence of the vaccine strain TV-M-310 of the vaccine Bioveta against ringworm.

We determined the intensity of growth, the ability to form spores and virulence in guinea pigs of the vaccine strain of the Czech live vaccine Bioveta. Comparative investigations were carried out with the vaccine strain of the Russian live vaccine LTF 130 and Trichophyton verrucosum field strains. Growth of the vaccine strains was faster than growth of the field strains on solid media and in broth. Later the vaccine strains formed many microconidia. In experimentally infected guinea pigs the virulence of the vaccine strain Bioveta was highly dependent on the infectious dose. At 10(1) CFU ml-1 (according to the number of CFUs in the Russian live vaccine) the vaccine strain Bioveta was nearly avirulent. Resuspended according to the manufacturer's instructions (at least 10(10) CFU ml-1) virulence of the vaccine strain Bioveta could be observed.

Animals↗