Biomedical subjects

U Stendahl

Publications and source records attributed to U Stendahl.

At least 55 records · Page 3Linked to original sources

PubMed1989

DNA determination in endometrial carcinoma by flow and image cytometry.

Endometrial carcinomas from 29 patients were analyzed by flow and image cytometric techniques with respect to DNA-content. Good agreement of ploidy value was obtained in 22 of the cases. A correlation was demonstrated between percentage cells above 2.5 C in image cytometry and S-phase as determined by flow cytometry. The interpretation was that cells with major chromosomal abnormalities may also have a high proliferative rate. These two features cannot be separated by image cytometry, whereas this is claimed to be the case according to the terminology used in interpretation of flow cytometric data. Our findings underline the need for a clarification of the terminology used in cell measurements on clinical material as well as proper choice of cytometric method to be used in conjunction with diagnostic cytology. The possible clinical value of the descriptors chosen from the two methods awaits follow-up of the patients.

DNA, Neoplasm↗

PubMed1989

Glioblastoma multiform in a dermoid cyst of the ovary.

Malignant transformation is reported in less than 2% of benign cystic teratomas. Although all the elements can undergo this transformation, it is most often seen in squamous epithelium. The malignancy of neural elements is probably the least common event, with only one case previously reported. A case of glioblastoma multiform in a benign cystic teratoma is presented. Its neural derivation was supported by an immunohistochemical staining specific for Glial Fibrillary Acidic Protein (GFAP). Despite very conservative surgery without adjuvant therapy, the patient remains alive and symptom free more than 3 years later.

Adult↗

PubMed1989

Second look laparotomy--its role in the management of ovarian carcinoma.

Almost 30 years after its introduction into the management of ovarian carcinoma, second-look laparotomy remains controversial. Although its supporters agree on its indications, there is a considerable number of conflicting reports on the actual benefits of the procedure. Diagnostic inaccuracy, invasiveness and expense are some of the reasons raised by opponents against its routine performance and their recommendations range from limited selective application to total avoidance. The role of second-look laparotomy is reviewed, with particular reference to developing countries where scarcity of resources must always be balanced against the need to provide the best care possible to the individual cancer patient. A management schedule for ovarian carcinoma, including second-look laparotomy, appropriate under these circumstances is given.

Africa↗

PubMed1988

DNA patterns and aggressive histopathologic features in 159 patients with cervical carcinoma.

In 159 patients with invasive squamous cell carcinoma of the uterine cervix, flow cytometric DNA patterns were related to eight histopathologic parameters according to a malignancy grading system, in which four of the parameters concern the tumor cell population and four concern the tumor-host relation. Each parameter was graded from 1 to 3 points. Flow cytometrically aneuploid values, contrasted to peridiploid ones, were more often found in specimens with immature, irregular nuclei, as well as in specimens with diffuse growth (p less than 0.05), obvious vascular invasion (p less than 0.05) or slight or no plasmolymphocytic response (p less than 0.05). For each histopathologic parameter, higher mean S-phase rates were found for the aggressive 3-point tumors than for the 1-point tumors. Tumors with total scores of 11-17 points were more often peridiploid than tumors with 18-22 points.

Adult↗

PubMed1988

Haptoglobin groups in ovarian carcinoma.

Haptoglobin groups were determined in 182 patients with primary ovarian carcinoma. Previously reported associations could not be confirmed. A significant excess of HP2-1 was observed among patients with a family history of cancer.

Female↗

PubMed1987

Automatic analysis of Papanicolaou smears by digital image processing.

Papanicolaou smears from 378 patients were analyzed in a fully automated microscope system using an image analysis technique. The logic of the system subdivided the smears into "normal" (63%), "not possible to analyze in the machine" (17%) and "positive" (20%). The results have been compared with the conventional screening method available today taking into consideration the definition problems of a true diagnosis, cytopathological and histopathological consensus, reproducibility, sample-taking and interpretation mistakes, etc. As 37% (rejected and positive) of the smears in this particular study were analyzed visually after machine prescreening, it could be demonstrated that the false-negative rate among the remaining 63% of the specimens was significantly lower among the machine-screened smears when compared with visual screening if the results were correlated with histopathological consensus. The results clearly indicate that the technology required to build computerized microscope systems, which are able to automatically sort out at least two thirds of Papanicolaou smears is available today. The only limiting factor is the cost-benefit relationship.

False Negative Reactions↗

PubMed1986

Comparative pharmacokinetic study of adriamycin and 4'epi-adriamycin after their simultaneous intravenous administration.

The plasma pharmacokinetics of adriamycin and 4'epi-adriamycin have been studied in 6 patients with ovarian carcinoma after simultaneous intravenous administration of equal amounts of the two anthracyclines. A highly selective liquid chromatographic analytical method permitted quantification of plasma concentrations of the two drugs as well as their corresponding 13-hydroxy metabolites. The plasma concentrations of each drug followed a three-compartment open model, with great interindividual variation in the pharmacokinetic parameters. On average, the area under the plasma concentration time curve (AUC) and the maximum plasma concentration (Cmax) were 1.6- and 1.2-times larger for adriamycin than for 4'epi-adriamycin. 4'Epi-adriamycin was eliminated faster than adriamycin by 4 of the 6 patients, the average terminal half-life of the latter being 1.4-times longer. The plasma concentrations of the 13-hydroxy metabolites did not exceed 20 ng/ml. Their AUC values averaged 23% of those of the intact drugs.

Adult↗

PubMed1985

Urinary modified nucleosides as tumor markers in cancer of the urinary organs or female genital tract.

Using a sensitive and specific method involving high-performance liquid chromatography, urinary levels of four modified nucleosides--pseudouridine (psi), 1-methylinosine (m1I), 1-methyladenosine (m1A), and 1-methylguanosine (m1G)--were investigated before and after treatment in 31 patients with cancer of the urinary organs or the female genital tract. Before treatment m1I was the most frequently elevated nucleoside (77%). Pretreatment urinary levels of psi, m1I, and m1A in patients with stage 2-4 cancer of the female genital tract were significantly elevated compared to human healthy volunteers (p less than 0.005). Compared with the other nucleosides, psi appeared to correlate more closely with the clinical outcome (progression or regression) of patients with cancer of the female genital tract. In the case of patients with cancer of the urinary organs, m1I followed the clinical outcome better than the other nucleosides measured. Therefore psi and m1I seem to be useful for monitoring genito-urinary cancers.

Adenosine↗

PubMed1983

Bleomycin-mitomycin C in advanced carcinoma of the cervix. A third look.

Thirty-three patients with advanced cervical cancer (31 squamous cancer, two adenosquamous cancer) previously untreated with cytotoxic drugs, were treated with bleomycin, 5 mg daily, for seven days and mitomycin C, 10 mg, on day 8. This regimen was repeated four times at two-week intervals. All but one patient had previously been treated with radiotherapy; 36% of the patients had an objective response (five complete remission (CR), median duration 12 months; seven partial remission (PR), median duration six months). Severe myelosuppression occurred in nine patients. One drug-related death due to thrombocytopenia occurred. Three patients developed pulmonary fibrosis and one of them died of respiratory failure. The bleomycin-mitomycin C regimen has a definite but clearly limited effect in advanced cancer of the uterine cervix.

Adult↗

PubMed1983

Prognosis of invasive squamous cell carcinoma of the uterine cervix: a comparative study of the predictive values of clinical staging IB--III and a histopathologic malignancy grading system.

The prognostic values of clinical staging IB--III and a histopathologic malignancy grading system have been compared in a retrospective study on 338 women with invasive squamous cell carcinoma of the uterine cervix. The superiority of the grading system to staging was significant (p less than 0.01). However, staging had an additional prognostic value. Using both the malignancy grading system and clinical staging together provided a better capacity to predict the clinical outcome in individual patients. Prognostication according to clinical staging alone will result in a considerable loss of information.

Adult↗

PubMed1983

Malacoplakia of the cervix and corpus uteri: a light microscopic, electron microscopic, and X-ray microprobe analysis of a case.

Malacoplakia in the female genital tract is rare. A case of a 71-year-old woman with malacoplakia of the cervical mucosa and endometrium is described. By light microscopy, von Hansemann cells containing calcified bodies (Michaelis-Gutmann bodies) could be visualized. Similar formations could also be seen extracellularly. Typical Michaelis-Gutmann bodies with electron-dense areas, as well as with pale centers and a dark periphery, could be identified by electron microscopy. Occasional trilaminar bacteria were seen. X-ray microanalysis indicated the presence of silica, sulfur, chloride, calcium, magnesium, and iron. Malacoplakia in this region may cause diagnostic problems for the pathologist, but the presence of a monotonous tumor-like infiltrate of pale histiocytes should lead to a careful search for Michaelis-Gutmann bodies.

Aged↗

PubMed1983

Invasive squamous cell carcinoma of the uterine cervix. VII. Influence of vehicle osmolarity on biopsy quality.

A histopathologic grading system for more reliable determination of the prognosis in individual patients with squamous cell carcinoma of the uterine cervix necessitates better sampling and fixation techniques. Fixation in 10 per cent formalin and 0.17 mol phosphate buffer at pH 7.0, with an effective osmolarity of 350 mmol/l, gave optimal stainability, and minimized tissue shrinkage and sectioning artefacts. Vessels remained open, which facilitated the analysis of vascular invasion. A newly designed biopsy forceps yielded large and representative specimens with a minimum of pressure distortion.

Biopsy↗

PubMed1982

Invasive squamous cell carcinoma of the uterine cervix. V. Late local recurrences after radiation therapy.

In 26 patients with late local recurrent invasive squamous cell carcinoma of the uterine cervix, primary and recurrence biopsies have been compared with respect to a malignancy grading system. In patients with 16 points or more (n = 13) there was no significant change between estimations. In patients scoring less then 16 points (n = 13), 7 patients had a significant rise in tumour host score indicating the possibility of new prognostic factors occurring. The concept of recurrence is discussed against the background of these findings.

Adult↗

PubMed1982

Invasive squamous cell carcinoma of the uterine cervix. VI. Prediction value of non-keratinizing, parakeratotic and orthokeratotic cell forms and clinical staging.

In 393 patients with invasive squamous cell carcinoma of the uterine cervix stages I to IV the predictive value of the histopathologic classification of Reagan & Wentz (differentiation into cell type) was analysed in relation to 10-year lethality rate. Patients with large cell horn-pearl forming and orthokeratinizing tumours had the poorest, and those with large cell non-keratinizing the best prognosis. Large cell parakeratotic tumours did not differ significantly in prognosis compared with the non-keratinizing cell form. When clinical staging was included the prediction value of the histopathologic classification disappeared except in stage II. From a clinical point of view this additional prognostic information will have little practical consequences.

Adult↗

PubMed1981

Cryosurgical treatment of cervical intraepithelial neoplasia with follow-up of five to eight years.

During 1972 to 1974, 181 women of different ages with cervical intraepithelial neoplasia were treated with cryosurgery. Of these, 170 have now been observed for 5 to 8 years. Treatment failures total 16%, but when the different age groups are considered the results are seen to be much better in young women less than 30 years of age, with only a 12% rate of failure, whereas older women have almost twice as many failures. One patient, 45 years of age, developed an early invasive cancer, but in her case pretreatment diagnosis may have been correct. Our conclusions are that cryosurgery is an easy and effective method of treating cervical intraepithelial neoplasia in young women, provided that the diagnosis prior to therapy is correct. Furthermore, we stress that after treatment the status of the patient must be checked with vaginal smears over a long period and also that at lease one endocervical curettage and directed biopsies of the vaginal cervix should be done as a control.

Adolescent↗

PubMed1981

Invasive squamous cell carcinoma of the uterine cervix. III. A malignancy grading system for indication of prognosis after radiation therapy.

The predictive value of a malignancy grading system embodied by 8 histopathologic parameters and of staging, age, year of admission and the different treatment modalities were compared in relation to 5- and 8-year survival. The material consisted of 196 patients in stage II according to the FIGO classification. The grading system proved superior with high significance (p less than 0.001) to the other predictors including each histopathologic parameter apart from vascular invasion (p = 0.01). All histopathologic parameters but 2 (differentiation into cell type and mitosis) exhibited significant (p less than 0.01) decrease in survival with higher grade at the 5- and 8-year controls. Patients in stage II could be divided into groups with low, intermediate and high malignancy points. Both groups of extremes showed a marked and highly significant difference in survival rates compared with the intermediate group, and the grading system made it possible to predict 60 per cent of the patients with acceptable specificity. In the group of high malignant tumours the system maintained a continual prognostic value even among patients still alive at the 5-year control.

Adult↗

PubMed1981

Invasive squamous cell carcinoma of the uterine cervix. IV. Analysis of a histopathologic malignancy grading system and construction of a partial index.

A new histopathologic malignancy index has previously proved capable of permitting discrimination of patients with carcinoma of the uterine cervix (stage II) with respect to prognosis. Eight histopathologic items graded from 1 to 3 add up to constitute the malignancy index. By regression analysis the number of items could be reduced to 4. Seemingly without loss in the prediction value a partial index was elaborated by the following 4 items: mitosis, mode of invasion, cellular response, and vascular invasion, the latter attributed double the weight. The predictive value of the partial index seemed to be as good as that of the previous malignancy index on an independent materials as well.

Carcinoma, Squamous Cell↗

PubMed1981

Invasive squamous cell carcinoma of the uterine cervix. II. Reproducibility of a histopathologic malignancy grading system.

A histopathologic malignancy grading system for invasive squamous carcinoma of the uterine cervix has previously been presented. The grading results in a total malignancy point value for every patient (range 8-24). This score has been proven to have a significant prognostic value for the individual patient. The inter-observer reproducibility of this system has been examined by two pathologists, on the pretreatment biopsies of 100 patients and the intra-observer reproducibility on 190 patients (observer one) and on 100 patients (observer two). These biopsies were estimated twice and three times, respectively, without knowledge of former results and at an interval of 2 to 6 months. The inter-observer correlation coefficient was 0.54, the intra-observer correlation coefficients 0.94 and 0.74, respectively.

Carcinoma, Squamous Cell↗