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U Taborski

Publications and source records attributed to U Taborski.

35 records · Page 2Linked to original sources
PubMed1995

Multicenter evaluation of a new capillary blood prothrombin time monitoring system.

The analytical performance of the new capillary blood prothrombin time monitoring system CoaguChek was examined in a multicenter evaluation at six hospitals. The coefficients of variation of the INR obtained in the CoaguChek imprecision study were approximately 7% in the control plasma provided (within-run and day-to-day) and 4% in blood (within-run). The prothrombin times were ascertained in capillary blood (CoaguChek PT Test) and citrated venous plasma (Hepato Quick, Thromborel S) from 359 patients under oral anticoagulation therapy with phenprocoumon, acenocoumarin or warfarin. The agreement with the test results obtained with the comparison methods was acceptable versus Hepato Quick assay (n = 359; y = 1.23 x -0.49, r = 0.888) and good versus Thromborel S method (n = 359; y = 1.09 x -0.28, r = 0.895). A simplified assessment of all test results (n = 795) in a nine-field comparison table showed a concordance with the comparison methods of more than 80 (Hepato Quick: 81%, Thromborel S: 83%). The concordance between Hepato-Quick and Thromborel S was slightly higher (88%). Its good analytical performance and convenient handling recommend the CoaguChek system as a suitable system for decentralized prothrombin time testing.

Blood Chemical Analysis↗
PubMed1994

[Blood component separation with gravity filtration: production of leukocyte-depleted erythrocyte concentrates].

In this study a leukocyte-depleted red-cell concentrate (RCC) was prepared by a new plasma separation filter separating by gravity. This separation filter and a leukocyte depletion filter were integrated in a 4-blood-bag system. The filtered RCC was characterized, the RCC was stored for 6 weeks, and tests to evaluate the quality of the stored RCC were performed every week. The leukocyte contamination of the RCC was (0.59 +/- 0.28 microliter-1) immediately after filtration. Compared with conventionally prepared RCC, at the end of the 6-week storage period low concentrations of LDH (86.0 +/- 5.5 U/l), K+ (36.6 +/- 6.4 mmol/l) and free hemoglobin (101.0 +/- 12.4 mg/100 ml) in the supernatant were determined.

Cytapheresis↗
PubMed1992

Application of an automated plasma filtration device and a blood monitoring system for LDL apheresis.

This study describes the adaptation of a filtration separation device (AK 10 from Gambro, München, FRG) and a blood monitor system (BTS 100 from Diamed, Köln, FRG) to the automated adsorption/desorption device for LDL apheresis. Plasma separation by filtration was performed by a hollow-fiber filter (OP5, Asahi Corp., Japan). In order to evaluate the quality of the plasma obtained by filtration, the platelet contamination, the activity of factors V and VIII and the concentration of the immunoglobulins IgG and IgM were determined. The following results were found: Platelet contamination of the plasma was 2,740/microliters. As compared to the patient's pretreatment values, activity of factor V was 94%, activity of factor VIII was 104%, concentration of IgG was 96%, concentration of IgM was 94%. Ten LDL aphereses were evaluated: red cell counts, platelet counts, hemoglobin concentration and hematocrit were not changed significantly. Thus, this new combination system represents an improved alternative to previously performed centrifugation methods.

Blood Cell Count↗
PubMed1992

Bedside monitoring of anti-coagulation under LDL apheresis by means of aPTT testing with a coagulation monitor.

Optimally adjusted anticoagulation under LDL apheresis is essential for successful treatment: Excessive anticoagulation exposes the outpatient to the risk of uncontrolled hemorrhage, insufficient anticoagulation may shorten the duration of utilization of the immune-adsorption columns. A new device (coagulation monitor 512, Ciba Corning) allows individual adaptation of dosage and timing of heparin application by modifying the standard schedule (5,000 IU intravenously before treatment, 2,000 IU/h continuously). APTT was measured before and after application of heparin and then at 30-min intervals both by the coagulation monitor and by conventional laboratory methods; the respective heparin concentration was determined in addition. The correlation coefficient between the heparin concentration and both the monitor-derived and the laboratory-derived aPTT was 0.811 and 0.590, respectively. An explanation for this finding might be that the monitor avoids the influences induced by subsequent collection and testing of samples associated with laboratory procedures.

Cholesterol, LDL↗
PubMed1992

[Preoperative autologous blood donation in heart surgery patients: laboratory parameters with oral iron substitution].

In this study we analyzed some hematologic parameters of 228 autologous blood donors at a cardiosurgic clinic. The standard schedule of drawing autologous blood at our clinic is 4 units whole blood (450 ml) and 1 unit plasma (750 ml) for a time of 5 weeks under iron substitution (equivalent to 240 mg Fe). In younger women this kind of drawing blood leads to an unacceptable decrease of hemoglobin concentration. The preoperative autologous blood donation results in an increase of erythropoiesis on the day of clinical admittance. The best parameters to supervise the hematologic situation of the outpatient autologous blood donors are the hemoglobin concentration and the hematocrit.

Adult↗
PubMed1991

[Autologous hemotherapy in heart transplantation: a case report].

The use of autologous blood in patients with organ transplantations is feasible only when the blood is stored in the frozen state, since the timing of the operation is not predictable. A 53 year-old patient with dilatative cardiomyopathy was selected for transplantation. By preoperative donation, four erythrocyte concentrates were prepared and frozen by high-glycerol low-temperature technique. The frozen blood was thawed, manually washed and made available for transfusion as required. The increment of hemoglobin after transfusion was 0.9 g/dl per unit. Quality control of the prepared erythrocyte concentrates was performed: Resistance to osmotic fragility of the erythrocytes was in the normal range, beginning hemolysis was found at 0.40 +/- 0.02% NaCl, complete hemolysis was found at 0.30 +/- 0.02% NaCl. The osmolarity of the supernatant fluid of the final washing was 0.314 +/- 0.116 mosmol/l. This case report stresses the feasibility to prepare and store autologous blood even if the time when the blood is needed cannot be determined.

Blood Component Transfusion↗
PubMed1990

[Procedure for producing a resuspended erythrocyte concentrate during technical plasmapheresis: infrequent donation with the same erythrocyte concentrate quality].

This procedure enables gaining an additional red cell concentrate (RCC) during a plasmapheresis (Autopheresis C, Baxter) by using a 3-blood-bag system and the additive solution Sag-M. The quality of the RCC and the fresh frozen plasma (FFP) is equivalent to those prepared from whole blood. This method is adapted to the ambulatory autologous blood donation in cases when more FFP than RCC is needed.

Blood Coagulation Factors↗
PubMed1986

Inhibitory effects of interferon-gamma on the T suppressor cell circuit in contact sensitivity.

The effects of a partially purified, splenocyte-derived murine interferon (MuIFN-gamma N) and a recombinant IFN-gamma (MuIFN-gamma R) on the T suppressor pathway and on the T effector cells of delayed type hypersensitivity were investigated in a 2,4-dinitrofluorobenzene contact sensitivity model. Various T cell subpopulations, suppressor T cells of afferent and efferent types, and an auxiliary T suppressor cells as well as a T effector cell of delayed type hypersensitivity were induced and the functions assessed in transfer experiments. Confirming the results of earlier experiments obtained with IFN-alpha, beta, the MuIFN-gamma N preparation and the rec. MuIFN-gamma R: enhanced the decreased response in animals sensitized with an antigen overload to an optimal response; inhibited the afferent-acting T suppressor cell in vivo and in vitro; inhibited the Ts-eff response; blocked the auxiliary T suppressor cell response after intravenous injection to recipients of Ts-eff cells on day 0 and 1; and did not suppress the activity of the T effector cell of delayed type hypersensitivity in vivo and in vitro (the MuIFN-gamma R was not tested). We conclude that IFN-gamma preferentially inhibited the T suppressor cell circuit of contact allergy. These results are similar to our observations on the inhibitory effects of a pure interferon-alpha, beta on the regulatory T suppressor cell circuit in contact allergy. Selective suppression of different T subpopulations by IFN-gamma may be an important regulatory mechanism in delayed type hypersensitivity.

Animals↗
PubMed1984

Inhibition of the T suppressor circuit of delayed-type hypersensitivity by interferon.

The effects of electrophoretically pure murine interferon (Mu-IFN-alpha beta) on the T suppressor pathway and on the T effector cell of delayed hypersensitivity (TDH) were investigated in BALB/c mice, in a 2,4-dinitrofluorobenzene (DNFB) contact-sensitivity model. Various T cell subpopulations, suppressor T cells of the afferent (Ts-aff) and efferent (Ts-eff) types, an auxiliary Ts (Ts-aux), as well as TDH were induced, and their function was assessed in transfer experiments. The results were as follows. At a dose of 5 X 10(3) U, IFN was shown to inhibit the Ts-aff response, when given to the donor animal shortly after induction of the Ts-aff subpopulation or when injected into the recipient 2 hr after spleen cell transfer. Pretreatment in vitro with IFN of the splenic cells to be transferred also abolished the Ts-aff response. Similar amounts of IFN were able to inhibit the generation of Ts-eff in the donor animals, whereas 10-fold-higher amounts were needed in vivo or in vitro to block the functional expression of Ts-eff in the recipient animal. Intravenous injection of IFN into recipients of Ts-eff on day 0 and 1 after sensitization inhibited the expression of the Ts-eff transferred 1 day before ear challenge. This suggests that the Ts-aux response required for the TDH suppression by Ts-eff is blocked by IFN. Secretion of a suppressor factor by Ts in vitro was not blocked by IFN. Treatment of the donor of suppressor factor-secreting Ts with IFN, however, blocked the induction of this Ts. The TDH were not sensitive to IFN even at amounts approximately 100 times higher than those used for the Ts inhibition in vivo as well as in vitro. These results demonstrate that low amounts of IFN may selectively block the suppressor pathway, because induction of these regulatory T cell subsets appears to be particularly sensitive to IFN. The exact mechanism of the IFN-mediated inhibition of Ts is not yet clear. The data suggest an important regulatory function of IFN in delayed-type hypersensitivity (DTH) reactions.

Animals↗
PubMedDate not supplied

Analysis of the long-term efficacy and selectivity of immunoadsorption columns for low density lipoprotein apheresis.

Immunoadsorption low density lipoprotein (LDL) apheresis is performed with reusable columns containing anti-apolipoprotein B(ApoB) antibodies. We analyzed their long-term efficacy and selectivity. Performance over 60 treatment sessions of six pairs of immunoadsorption LDL apheresis columns was evaluated by analysis of variance using the removal of total cholesterol and ApoB to assess efficacy and the ratio of total cholesterol/high density cholesterol removed to assess selectivity. The removal of cholesterol did not vary significantly with treatment number. The mass of ApoB removed increased significantly (p = 0.002), and the mass of ApoB removed per volume unit of processed plasma showed a trend (p = 0.065) toward an increase with treatment number. Both parameters correlated with the serum ApoB concentration before treatment, which also increased significantly (p = 0.0007) with treatment number. No significant variation of selectivity was found. The efficacy of the LDL apheresis immunoadsorption columns did not decrease after 60 treatment sessions. The columns' selectivity also remained unchanged.

Analysis of Variance↗